Active Recombinant Human EIF2AK3(536-end) Protein, N-His-tagged

• Cat.No.: EIF2AK3-189H
• Product Overview: N-terminal 6Histagged, recombinant, human PEK amino acids 536 – end, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: PEK is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability (By similarity).EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 536-end
• Form: 0.82mg/ml of enzyme in of 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 383U/mg, where one unit of PEK, active activity is defined as 1nmol phosphate incorporated into 300μM RSRSRSRSRSRSRSR per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 71 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQTTFI VRRLFHPHPH RQRKESETQC 61 QTENKYDSVS GEANDSSWND IKNSGYISRY LTDFEPIQCL GRGGFGVVFE AKNKVDDCNY 121 AIKRIRLPNR ELAREKVMRE VKALAKLEHP GIVRYFNAWL EAPPEKWQEK MDEIWLKDES 181 TDWPLSSPSP MDAPSVKIRR MDPFATKEHI EIIAPSPQRS RSFSVGISCD QTSSSESQFS 241 PLEFSGMDHE DISESVDAAY NLQDSCLTDC DVEDGTMDGN DEGHSFELCP SEASPYVRSR 301 ERTSSSIVFE DSGCDNASSK EEPKTNRLHI GNHCANKLTA FKPTSSKSSS EATLSISPPR 361 PTTLSLDLTK NTTEKLQPSS PKVYLYIQMQ LCRKENLKDW MNGRCTIEER ERSVCLHIFL 421 QIAEAVEFLH SKGLMHRDLK PSNIFFTMDD VVKVGDFGLV TAMDQDEEEQ TVLTPMPAYA 481 RHTGQVGTKL YMSPEQIHGN SYSHKVDIFS LGLILFELLY PFSTQMERVR TLTDVRNLKF 541 PPLFTQKYPC EYVMVQDMLS PSPMERPEAI NIIENAVFED LDFPGKTVLR QRSRSLSSSG 601 TKHSRQSNNS HSPLPSN
• Purity: Purity 81.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: EIF2AK3 eukaryotic translation initiation factor 2 alpha kinase 3 [ Homo sapiens (human) ]
• Official Symbol: EIF2AK3
• Synonyms: EIF2AK3; eukaryotic translation initiation factor 2 alpha kinase 3; PEK; WRS; PERK
• Gene ID: 9451
• mRNA Refseq: NM_004836.7
• Protein Refseq: NP_004827.4
• MIM: 604032
• UniProt ID: Q9NZJ5