Recombinant Human TNFRSF10B, Fc-tagged

• Cat.No.: TNFRSF10B-28127TH
• Product Overview: Recombinant fragment corresponding to amino acids 56-182 of Human DR5 fused to the Fc region of human IgG1 expressed in modified human 293 cells, 40-50kDa.

Specification

• Description: The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene.
• Conjugation: Fc
• Tissue specificity: Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLa S3, K562, HL-60, SW480, A549 and G361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, p
• Biological activity: The ED50 of DR5 Fc Chimera is typically 38-40 ng/ml as measured by its ability to neutralize TRAIL mediated cytotoxicity using the human leukemic Jurkat cells.
• Form: Lyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Trehalose, 1% Human serum albumin
• Storage: Shipped at 4°C. After reconstitution store at -20oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: Theoretical sequence:ITQQDLAPQQRAAPQQKRSSPSEGL CPPG HHISEDGRDCISCKYGQDYSTHWND LLFCLRCTRCDSG EVELSPCTTTRNTVCQCEEGTFREE DSPEMCRKCRTGC PRGMVKVGDCTPWSDIECVHKEGSS NTKVDKKVEPKSCDKTHTCPPCPAP ELLGGPSVFLFPPKPKDTLMISRTP EVT CVVVDVSHEDPEVKFNWYVDGVEVH NAKTKPREEQYNS TYRVVSVLTVLHQDWLNGKEYKCKV SNKALPAPIEKTI SKAKGQPREPQVYTLPPSRDELTKN QVSLTCLVKGFYP SDIAVEWESNGQPENNYKTTPPVLD SDGSFFLYSKLTVDKSRWQQGNVFS CSVMHEALHNHYTQKSLSLSPGK
• Sequence Similarities: Contains 1 death domain.Contains 3 TNFR-Cys repeats.

Gene Information

• Gene Name: TNFRSF10B tumor necrosis factor receptor superfamily, member 10b [ Homo sapiens ]
• Official Symbol: TNFRSF10B
• Synonyms: TNFRSF10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor superfamily member 10B; CD262; DR5; KILLER; TRAIL R2; TRICK2A; TRICKB
• Gene ID: 8795
• mRNA Refseq: NM_003842
• Protein Refseq: NP_003833
• MIM: 603612
• Uniprot ID: O14763
• Chromosome Location: 8p22-p21
• Pathway: Activation of Pro-Caspase 8, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem
• Function: TRAIL binding; cysteine-type endopeptidase activator activity involved in apoptotic process; protein binding; receptor activity

Reviews

08/11/2020

The combination of high-quality TNFRSF10B protein and excellent technical support provided by the manufacturer makes them the ideal choice for my research.

10/07/2018

The manufacturer's extensive experience and expertise in the field of TNFRSF10B protein research make them a trusted partner.

03/04/2017

The manufacturer's commitment to providing excellent technical support is highly valuable.

Q&As

How does TNFRSF10B activation induce apoptosis in cancer cells?

Activation of TNFRSF10B triggers apoptotic pathways, leading to programmed cell death in cancer cells.

Is TNFRSF10B involved in other diseases besides cancer?

TNFRSF10B has been implicated in certain autoimmune diseases and neurodegenerative disorders, highlighting its diverse roles in various physiological processes.

Can TNFRSF10B be used as a diagnostic marker for cancer?

TNFRSF10B expression levels may serve as a potential diagnostic marker for certain cancers, aiding in cancer detection and prognosis.

In what types of cancer is TNFRSF10B expression altered?

TNFRSF10B expression is often altered in various cancers, including breast cancer, colorectal cancer, and lung cancer.

Are there any side effects associated with targeting TNFRSF10B in cancer treatment?

Side effects may occur, and ongoing research aims to minimize these effects while maximizing the therapeutic benefits of targeting TNFRSF10B.