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Recombinant Human TNFRSF10B cell lysate

Cat.No. : TNFRSF10B-2829HCL
Product Overview : Human TNFRSF10B / TRAILR2 / CD262 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : TNFRSF10B tumor necrosis factor receptor superfamily, member 10b [ Homo sapiens ]
Official Symbol : TNFRSF10B
Synonyms : TNFRSF10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor superfamily member 10B; CD262; DR5; KILLER; TRAIL R2; TRICK2A; TRICKB; Fas-like protein; death receptor 5; cytotoxic TRAIL receptor-2; apoptosis inducing receptor TRAIL-R2; apoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; death domain containing receptor for TRAIL/Apo-2L; tumor necrosis factor receptor-like protein ZTNFR9; p53-regulated DNA damage-inducible cell death receptor(killer); TRICK2; ZTNFR9; TRAILR2; TRICK2B; TRAIL-R2; KILLER/DR5;
Gene ID : 8795
mRNA Refseq : NM_003842
Protein Refseq : NP_003833
MIM : 603612
UniProt ID : O14763
Chromosome Location : 8p22-p21
Pathway : Activation of Pro-Caspase 8, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis, organism-specific biosystem; Caspase-8 is formed from procaspase-8, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem;
Function : TRAIL binding; protein binding; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does TNFRSF10B activation induce apoptosis in cancer cells? 03/09/2023

Activation of TNFRSF10B triggers apoptotic pathways, leading to programmed cell death in cancer cells.

Is TNFRSF10B involved in other diseases besides cancer? 04/19/2021

TNFRSF10B has been implicated in certain autoimmune diseases and neurodegenerative disorders, highlighting its diverse roles in various physiological processes.

Can TNFRSF10B be used as a diagnostic marker for cancer? 03/08/2019

TNFRSF10B expression levels may serve as a potential diagnostic marker for certain cancers, aiding in cancer detection and prognosis.

In what types of cancer is TNFRSF10B expression altered? 03/19/2017

TNFRSF10B expression is often altered in various cancers, including breast cancer, colorectal cancer, and lung cancer.

Are there any side effects associated with targeting TNFRSF10B in cancer treatment? 06/06/2016

Side effects may occur, and ongoing research aims to minimize these effects while maximizing the therapeutic benefits of targeting TNFRSF10B.

Customer Reviews (3)

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Reviews
08/11/2020

    The combination of high-quality TNFRSF10B protein and excellent technical support provided by the manufacturer makes them the ideal choice for my research.

    10/07/2018

      The manufacturer's extensive experience and expertise in the field of TNFRSF10B protein research make them a trusted partner.

      03/04/2017

        The manufacturer's commitment to providing excellent technical support is highly valuable.

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