Active Recombinant Full Length Human MAPK10 Protein, N-His-tagged

Cat.No. : MAPK10-86HFL
Product Overview : N-terminal 6His-tagged, recombinant, full length human JNK3/SAPK1b, expressed by baculovirus Sf21 insect cells. Purified using Ni2+ -NTA agarose. Activated with MKK4 and MKK7β1 and repurified using Ni2+ -NTA agarose.
  • Specification
  • Gene Information
  • Related Products
  • Download
Description : c-Jun N-terminal kinases (JNKs), a stress-activated protein kinase (SAPK), is involved in cellular responses to environmental stresses. JNK kinases can be activated by stimuli such as UV light, radiation, protein synthesis inhibitors, ceramide, DNA-damaging drugs, chemopreventive drugs, TNF-a, and interleukin 1. JNK binds to an NH2-terminal region of ATF2 and c-Jun and phosphorylates two sites within the activation domain of each transcription factor. Three JNK genes (Jnk1, -2 and -3) have been identified in humans; however, splice variants result in a total of 10 isoforms. Inhibition of JNK3 (MAPK10, Mitogen-activated protein kinase 10, Stress-activated protein kinase JNK3) may provide clinical benefit in diseases as diverse as immune inflammatory (joint, bowel, pulmonary), ischemic injury (brain, cardiac) and neurodegenerative (Alzheimer's, Parkinson's) diseases.
Source : Sf21
Species : Human
Tag : His
Protein length : Full Length
Form : 2.76mg/ml of 50mM Tris/HCl, pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
Bio-activity : 128U/mg, where one unit of JNK3 activity is defined as 1nmol phosphate incorporated into 3μM ATF-2 (cat# 12-367) per minute at 30 centigrade with a final ATP concentration of 100μM.
Molecular Mass : 53 kDa
AA Sequence : 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSPG ISGVDVSSVA KHYNMSKSKV DNQFYSVEVG 61 DSTFTVLKRY QNLKPIGSGA QGIVCAAYDA VLDRNVAIKK LSRPFQNQTH AKRAYRELVL 121 MKCVNHKNII SLLNVFTPQK TLEEFQDVYL VMELMDANLC QVIQMELDHE RMSYLLYQML 181 CGIKHLHSAG IIHRDLKPSN IVVKSDCTLK ILDFGLARTA GTSFMMTPYV VTRYYRAPEV 241 ILGMGYKENV DIWSVGCIMG EMVRHKILFP GRDYIDQWNK VIEQLGTPCP EFMKKLQPTV 301 RNYVENRPKY AGLTFPKLFP DSLFPADSEH NKLKASQARD LLSKMLVIDP AKRISVDDAL 361 QHPYINVWYD PAEVEAPPPQ IYDKQLDERE HTIEEWKELI YKEVMNSEEK TKNGVVKGQP 421 SPSGAAVNSS ESLPPSSSVN DISSMSTDQT LASDTDSSLE ASAGPLGCCR
Purity : Purity 99% by SDS-PAGE and Coomassie blue staining.
Applications : Drug Discovery & Development
Storage : 6 months at -70 centigrade.
Handling Advice : Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
Use/Stability : On receipt of material store at - 70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.
SDS-PAGE: 13–69ng of this lot of enzyme phosphorylated 3μM ATF-2 in the assay.
Gene Name : MAPK10 mitogen-activated protein kinase 10 [ Homo sapiens (human) ]
Official Symbol : MAPK10
Synonyms : MAPK10; mitogen-activated protein kinase 10; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
Gene ID : 5602
mRNA Refseq : NM_139070.3
Protein Refseq : NP_620709.1
MIM : 602896
UniProt ID : P53779

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (0)

Ask a question

Customer Reviews (0)

Write a review

Ask a Question for All MAPK10 Products

Required fields are marked with *

My Review for All MAPK10 Products

Required fields are marked with *

0

Inquiry Basket

cartIcon
logo

FOLLOW US

Terms and Conditions        Privacy Policy

Copyright © 2024 Creative BioMart. All Rights Reserved.

Contact Us

  • /
  • Service lnquiry:

Stay Updated on the Latest Bioscience Trends