Bio-Layer Interferometry (BLI) Service

Biomolecular interactions are important for many biological processes; the development of biosensor technologies for the investigation of biomolecular interactions has markedly advanced over the last years. Label-free optical methods like Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) have been used in vitro to qualify and quantify protein-protein interactions by studying kinetics of binding and dissociation.

Bio-Layer Interferometry measures biomolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. In BLI experiment, one molecule is immobilized to a biosensor and binding to a second molecule is measured. The molecules that bind or dissociate themselves from the biosensor causes a shift in the interference pattern that is measured in real-time. The unbound molecules, variations in the refractive index of the surrounding medium or flow rate, however, do not affect the interference. This unique feature of BLI technology therefore allows measuring crude samples to determine the kinetics and affinity of molecular interactions. Anotheradvantage of BLI is that the number of sensors can be scaled up easily without making the system more error-prone or complex. Theoretically, there is no need to regenerate single sensors, because immobilizing an equal amount of ligand on additional sensors can easily create duplicates of the surface.

 

 

 

  • Fast and easy to operate
  • Label-free and real-time detection
  • Complete kinetic characterization of a molecular interaction
  • Wide range of binding affinity measurable (nM to mM)
  • Wide range of possible buffers and solvents
  • Possibility to measure in crude biological liquids or samples
  • Wide temperature range
  • Wide range of possible molecules and ligands
  • Immune to fluctuations in refractive index of samples
  • No microfluidics involved
  • Less expensive

Creative BioMart assists scientists to accelerate their research progress in a highly productive and cost-effective way. Creative BioMart’s Bio-Layer Interferometry platform provides a one-stop solution for researchers who have expressed interests in macromolecular assembly, membranes, liposome, viral like particles and binding of peptides to proteins. BLI can be applied to a wide pharmaceutical field of protein quantitation, affinity and kinetics analysis, drug/antibody screening, drug/antibody discovery, and optimization of drug process development.

 

Bio-Layer Interferometry Applications
Kinetic applications Quantitative applications
Protein-protein interactions
Antibody characterization
Antibody-antigen binding
Protein-small molecule interactions
DNA-aptamer binding
Bacteria-antibody interactions
Virallike particle-antibody/protein binding
GPCR-protein binding
Antibody quantitation
ELISA replacement
Protein quantitation in crude extracts
Detection of contaminations
Screening applications Assay development applications
DNA-aptamer screening
Small molecule fragment screening
Secondary screening and hit validation
Inhibitor screening
Formulation development
Media development

 

Reference:

1. Christian Margreitter,PatrickMayrhofer, Renate Kunert, Chris Oostenbrink.Antibody humanization by molecular dynamics simulations—in-silico guided selection of critical backmutations. Journal of Molecular Recognition. Jan 2016 DOI: 10.1002/jmr.2527

2. Krista Witte. Fluidics-Free Molecular Interactions Analysis.Genetic Engineering&Biotechnology News. July 01, 2009 (Vol. 29, No. 13)

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