Protein Expression Microarray

Creative BioMart has a high throughput protein microarray technique that enables measurements of protein expression levels in hundreds of biological samples. Proteins extracted from cell lysates, bio-fluids or fixed-tissues are immobilized as spots in rows on a solid support. Each micro-spot holds the entire proteome of a sample. Highly specific antibodies are used to simultaneously screen these spots for the level of distinct target proteins.

Advantages:

• High throughput method using automation
• Microvolumes of sample (5-10 µl)
• Convenient cost

Key advantages against Western Blot analyses:

	Creative BioMart Protein expression Microarray	Western blot
Speed and costs	-until 500 sample per slide -until 16 antibodies per slide -Low amount of sample needed (5ul to test hundreds of targets simultaneously)	-until 10 sample per gel -1 antibody per gel -Significant amount of sample needed (10ul for each target to test)
Data quality	-Accuracy 5% -High reproducibility (C.V. around 5%)	-Accuracy 20% -Medium reproducibility (C.V. around 25%)

Price on request, please contact us for a quote or for further information.