Protein Phosphorylation Site Identification and Mapping Service

Creative BioMart provides for the detection of phosphosites located in a target protein that has been phosphorylated in vivo and purified by immuno-precipitation or a recombinant protein that has been phosphorylated in vitro with a purified protein kinase.

Our speciality is the identification of protein phosphorylation sites. We usually identify phosphorylation sites by collision induced dissociation (CID) or electron-transfer dissociation (ETD) in our systems.

We also perform a number of 32P labelled phosphorylation site analyses. Here, radiolabelled proteins are digested with a protease and peptides separated on an off-line HPLC system with an on-line radioactivity detector. Peaks containing 32P are identified by a combination of mass spectrometry and solid phase Edman degradation. This methodology has been used in a large number of publications from the Unit describing novel protein phosphorylation sites and our lab is regarded as one of the world's experts in this technology.

Protein Phosphorylation Site Identification and Mapping Service

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