Protein Extraction Services

Cell lysis is the first step in cell fractionation, organelle isolation, protein extraction and purification. Many techniques have been developed to obtain the best possible yield and purity of target molecules or subcellular structures from different sample types (cells or tissue) and species of organisms.

With over 20 years of experience in isolating proteins from tissues/cells of all kinds of species, including animal, plant, bacteria, yeast, etc., we offer the premium quality extraction service with our proprietary techniques. Creative BioMart provides several systems to simplify protein isolation and purification tasks. Affinity chromatography resins are available for the purification of tagged proteins and antibodies. Magnetic beads are developed for fast immunoprecipitation and isolation of your targets.

Types of Proteins:

• Enzymes
• Growth factors
• DNA binding proteins
• Blood proteins
• Membrane proteins
• Mitochondrial proteins
• Chloroplast proteins

• Organelle protein extraction
• Nuclear protein extraction
• Membrane protein extraction
• Cytoplasmic protein extraction

Click here to find our product list of native purified proteins.

Depending on the requirements and nature of the protein, we are able to design specialized approach for extraction. We will purify the protein cost-efficiently, characterize it according to your specifications and deliver it to you in an active and application-compatible form. Purification protocols specified by our clients or obtained from the literature can also be followed at our site.

Extraction modes	Protocols	We deliver	Advantages
Ion-exchange Gel filtration Affinity (broad-spectrum) Hydrophobic interaction	Client-supplied protocols Published protocols Improved protocols developed by Creative BioMart Protocols tailored to the client's requirements	Purified protein sample Specified protein concentration Special formulation to prevent protein from degradation Custom aliquoting as specified by customer Certificate of analysis as specified by customer	Strong expertise in protein technologies Competitive timeline Open pricing policy Performance under strict confidentiality Close attention to client's needs and requirements Weekly project updates Continued technical support after project completion

These unique extracts are applicable in a wide variety of functional assays in vitro, such as:

• Pre-mRNA splicing assays
• Polyadenylation assays
• Transcription assays
• Translation assays

• Kinase assays
• Gel shift assays (protein-DNA/RNA interactions)
• Protein-protein interaction assays
• Western blot assays