Protein Characterization

Analytical Protein Characterization ensures the identity, purity, structural and conformational integrity and activity of a protein. The precise amino acid sequence, molecular weight, charge variances, glycosylation, aggregation level, and oxidation level are all key components of thorough characterization of recombinant protein.

Creative BioMart performs a number of routine protein analyses throughout all project stages to understand the heterogeneity, impurity profile, and potency of a recombinant protein destined for pharmaceutical use. We have developed the platforms and analytical methods to pursue a comprehensive chemical, physical, and conformational characterization.

Preparation and submission of protein samples for

• Protein identity, purity and impurities
• Charge isoforms
• Oxidation
• Aggregation analysis
• Amino acid analysis
• Peptide mapping
• Glycan analysis and profiling

Platform and Analytical Techniques

Protein Identity, Purity and Impurities SDS electrophoresis Analytical Size-Exclusion Chromatography Chip based Protein Electrophoresis Assays Caplillary electrophoresis UV detection HPLC Charge Isoform Gel Isoelectric Focusing Capillary Isoelectric Focusing Whole-Column Isoelectric Focuing OFFGEL electrophoresis Ion Exchange Column-HPLC Aggregation analysis SDS PAGE Chip-based Protein Electrphoresis Size Exclusion HPLC Light Scattering Oxidation HPLC-LC/UV Reversed-Phase LC/MS Enzymatic digestion + LC/MS

 

Amino Acid Analysis OPA derivitization + RP-LC/UV FMOC derivitization + RP-LC/UV Derivitization + RP-LC/MS Peptide Mapping RP-LC/UV RP-LC/MS Capillary electrophoresis detection by DAD (diode array detection) or MS Glycan Profiling HPLC/fluorescence detection CE/fluorescence MALDI-TOF/MS LC/MS HPLC/pulsed amperiometric detector

To find out more about protein characterization services for biologics, contact us immediately.