Principle & Protocol

Complete step-by-step protocol for detergent selection and optimization in membrane protein purification. Covers buffer design with lipids and stabilizers, detailed solubilization workflows, detergent removal methods, chromatography integration, and comprehensive troubleshooting with decision trees. Includes practical guidelines for DDM, LMNG, SDS, CHAPS, and other detergents to maximize yield and preserve protein activity.

Complete step-by-step protocol for membrane protein purification using ionic detergents. Covers cell harvest to quality control with detergent screening, solubilization, IMAC, detergent exchange, SEC, and comprehensive troubleshooting. Detailed methodology for maximizing yield and preserving protein activity.

This protocol provides a robust and efficient method for in vitro biotinylation of Avi-tagged proteins.

This guideline aims to explain the method of separating peptide targets using a liquid chromatography column after pancreatic enzyme hydrolysis, and then detecting them through MALDI-TOF-TOF mass spectrometry.

The purpose of this manual is to help scientific researchers understand the mass spectrometry method, master the basic principle of matrix assisted laser desorption ionization time-of-flight mass spectrometry and the mass spectrometry identification method of gel separation samples, and finally be able to use GPS Explorer software to analyze the mass spectrometry data.

This experimental manual aims to help scientific researchers master the basic principles and main steps of mass spectrometry identification of phosphorylated peptides and phosphorylated sites, and understand the methods and applications of mass spectrometry identification of phosphorylated peptides and phosphorylated sites.

This lab manual is designed to help researchers master the basic principles and main steps of strong cation exchange chromatography for the enrichment of phosphorylated proteins and understand the methods and applications of strong cation exchange chromatography for the detection of phosphorylated proteins.

This lab manual is designed to help researchers master the basic principles and main steps of enrichment of phosphorylated peptides by solid-phase metal ion affinity chromatography and understand the methods and applications of enrichment of phosphorylated peptides by solid-phase metal ion affinity chromatography.

This experimental guide aims to help scientific researchers master the basic principle and main steps of affinity enrichment method for detecting protein phosphorylation level, and understand the application of affinity enrichment method for detecting phosphorylated protein.

This experimental manual is designed to help scientific researchers master the basic principles and main steps of western blot detection of protein phosphorylation level, and understand the application of phosphorylation antibody detection methods.