Fucose-free Protein Expression System

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Fucose-free Protein Expression System

Fucose is a hexose deoxy sugar with the chemical formula C6H12O5. It is found on N-linked glycans on the mammalian, insect and plant cell surface, and is the fundamental sub-unit of the fucoidan polysaccharide. α-1,3 linked core fucose is a suspected carbohydrate antigen for IgE-mediated allergy.

Fucose-free Protein Expression System

In the fucose-containing glycan structures, fucosylated glycans, fucose can exist as a terminal modification or serve as an attachment point for adding other sugars. In human N-linked glycans, fucose is most commonly linked α-1,6 to the reducing terminal β-N-acetylglucosamine. However, fucose at the non-reducing termini linked α-1,2 to galactose forms the H antigen, the substructure of the A and B blood group antigens.

Creative BioMart has developed the Fut8-/- CHO-K1 cell line, which can give the protein production lower cytotoxicity. It was mainly used in the production of biomedical drug and biosimilar development. Using Fut8-/- CHO-K1 cell lines expressing protein production, it makes the drug more activity than the same traditional drugs.

Fucose-free protein not only significantly improved anticancer efficacy and reduced side effects, but also greatly reduced the cost of production. Fucose-free protein development is the main development trend of global biopharmaceutical industry at present.

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