Caspase Inhibitors Proteins

Home / Cancer Proteins / Invasion microenvironment Proteins / Apoptosis Proteins / Caspase & inhibitor Proteins / Caspase Inhibitors Proteins

Caspase Inhibitors Proteins

Creative BioMart Caspase Inhibitors Proteins Product List
Caspase Inhibitors Proteins Background

Caspase is a key effector of a physiological death process called apoptosis, although some are involved in cytokine activation rather than cell death. They are present in most of our cells as inactive precursors (zymogens), and once activated, they kill the cells. Caspase can be controlled in two ways. The processing and activation of caspase can be regulated by molecules such as FADD, APAF-1, members of the Bcl-2 family, FLIP and IAP. Active caspase can be controlled by a variety of inhibitors that directly interact with proteases.

Caspase Inhibitors Proteins Figure 1. Protein structure of Caspase.

Viral and cellular caspase inhibitors

The virus must choose a cell's synthetic machinery to reproduce. Therefore, the most effective way to prevent virus replication is to have infected cells kill themselves. However, just as apoptosis is used to defend against viruses, viruses carry cell death inhibitors to prevent this response from their host cells. Several viral cell death inhibitors work by binding to activated caspase. The first caspase inhibitor identified was the vaccinia virus product cytokine response regulator A (CrmA), which was found to inhibit caspase 1 (interleukin 1b converting enzyme or ICE). The role of death has been previously identified by crmA, so it can be speculated that the virus uses CrmA to bind to active caspase 1 to reduce the defensive inflammatory response triggered by IL-1b. Although it is true, the identified caspase is a key mediator of apoptosis, and it has been clearly found that CrmA can be used to prevent defensive suicide in infected cells, leaving more time for virus replication. These observations also highlight the close connection between inflammation and defensive apoptosis.


CrmA can bind to and inhibit Caspase 1 with an inhibitory constant (Ki) of *10 picomolar and Caspase 8 with a Ki of less than 300 pM. In this way crmA can prevent production of mature IL-1b and IL-18 by Caspase 1, and apoptosis triggered by TNF receptor family members, which is mediated by Caspase 8.4 – 6 The inhibitory constants indicate crmA is unlikely to inhibit caspases 3, 6, 7 in vivo whilst there is some discrepancy in results regarding Caspase 10.8,9 Although it inhibits caspase 9 with a Ki of 2 nM in vitro, the fact that lymphocytes from Caspase 9 knock out animals were resistant to dexamethasone, but lymphocytes from transgenic mice expressing crmA were not,10,11 suggests crmA cannot inhibit Caspase 9 in vivo.


p35 is a baculovirus protein that blocks insect cells' defensive apoptotic response to viral infections. 19p35 not only inhibits several insect caspases, but also inhibits C. elegans CED-3 and mammalian cysteine proteases 1, 3, 6, 7, 8, and 10 with Ki values less than 10 nM,but does not inhibit non-cysteine cysteine proteases or serine proteases. After caspase cleaves its P1 residue (Asp87), p35 forms a complex that can be dissociated by SDS. 20 Since the cleaved subunit of p35 is still inhibited along with the cysteine protease, p35 can fundamentally function in different ways to display crmA.


The closest mammalian homologue of crmA is the serine protease inhibitor leukocyte elastase inhibitor, but the active (P1) site of this serine protease inhibitor has no acidic residues and therefore cannot inhibit caspase. Cytotoxic lymphocyte serine protease inhibitor 9 (PI-9) (also known as granzyme B inhibitor (GBI)) is also a close relative of crmA, but it has acid residues in vertebrate serine protease inhibitors in its pseudo substrate region.   Although this is a glutamate residue in PI-9 and not an aspartic acid found in crmA, it increases the likelihood that PI-9 can inhibit caspase. In in vitro experiments, it was found that PI-9 does not inhibit caspase, but can inhibit granzyme B. At the same time, it can prevent granzyme B-mediated apoptosis, but it cannot kill CD95 activated by Caspase 8 Pathway stimulates cells.


  1. PG Eker, et al.; Caspase inhibitors. Nature Publishing Group, 1999, 6.

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy