apx2
Species | Cat.# | Product name | Source (Host) | Tag | Protein Length | Price |
---|---|---|---|---|---|---|
Arabidopsis Thaliana | APX2-1271A | Recombinant Arabidopsis Thaliana APX2 Protein (4-250 aa), His-tagged | E.coli | His | 4-250 aa |
- Q&As
- Reviews
Q&As (27)
Ask a questionAPX2 can interact with various proteins to form protein-protein complexes and participate in different cellular processes. For instance, APX2 has been reported to interact with heat shock proteins (HSPs), thioredoxins, and other antioxidant enzymes like catalase and superoxide dismutase. These interactions can regulate APX2 activity, stability, or subcellular localization, and contribute to the overall antioxidant defense system in plants.
APX2 shows tissue-specific expression patterns, with differential expression in different plant tissues and developmental stages. It is commonly found in various aerial tissues, such as leaves, stems, and flowers, where it plays a crucial role in protecting plant cells from oxidative damage. In contrast, its expression levels in roots or other specific tissues may vary depending on the plant species and environmental conditions.
The subcellular localization of APX2 is determined by specific targeting signals present in its amino acid sequence. These signals guide APX2 to specific organelles, such as the cytosol, chloroplasts, mitochondria, or peroxisomes, where it is needed for efficient detoxification of reactive oxygen species in those compartments.
Multiple molecular techniques have been employed to study APX2. Some common techniques include reverse transcription polymerase chain reaction (RT-PCR) for gene expression analysis, gene cloning and transformation to generate APX2 mutants or transgenic plants, protein purification and activity assays to evaluate APX2 function, immunoblotting for protein detection and quantification, and chromatin immunoprecipitation (ChIP) to study APX2 transcriptional regulation.
Yes, researchers have generated APX2 mutants or genetically modified plants with altered APX2 expression or activity. These studies have helped elucidate the role of APX2 in stress tolerance, plant development, and overall plant physiology. Mutants with reduced APX2 expression often show increased susceptibility to oxidative stress or altered growth phenotypes.
Although APX2 is primarily studied in plants, its role in antioxidant defense and stress tolerance mechanisms can have implications in human health and biotechnology. Understanding the molecular mechanisms of APX2 and related antioxidant enzymes can contribute to the development of antioxidant-based therapies and the engineering of stress-tolerant crops.
While APX2 is primarily studied in plants, its potential therapeutic applications in human health have not been extensively explored. However, its antioxidant function and ability to scavenge reactive oxygen species suggest that it could be a target for developing antioxidant therapies or protective measures against oxidative stress-related diseases.
Understanding the molecular mechanisms of APX2 and its involvement in stress tolerance can help in the development of more resilient and stress-tolerant crop varieties. By manipulating APX2 expression or activity, it may be possible to enhance crop yields and improve agricultural practices, particularly in regions prone to environmental stresses.
APX2 expression can be induced by various stress-responsive transcription factors, including AP2/EREBP, WRKY, and MYB. These transcription factors bind to specific regulatory regions of the APX2 gene, known as cis-elements, promoting its transcription in response to stress signals.
Yes, APX2 can interact with various proteins involved in redox signaling, stress response, and cellular processes. It can associate with regulatory proteins like mitogen-activated protein kinases (MAPK), transcription factors, and other enzymes involved in antioxidant defense, forming complexes that regulate APX2 activity or modulate downstream responses to stress signals.
While specific inhibitors of APX2 are less well-studied, compounds such as salicylic acid have been shown to enhance APX2 activity and expression in plants, resulting in increased stress tolerance. Additionally, various factors and signaling molecules, including reactive oxygen species, nitric oxide, and phytohormones, can modulate APX2 activity and expression.
APX2 can undergo various post-translational modifications, such as phosphorylation, acetylation, and ubiquitination, which can regulate its activity, stability, or subcellular localization. These modifications can impact APX2's ability to respond to stress signals and modulate its antioxidant function.
Yes, plants genetically modified to overexpress APX2 have been shown to have enhanced stress tolerance. They exhibit improved ROS scavenging ability, increased antioxidant enzyme activities, and reduced oxidative damage, leading to better survival and growth under stress conditions.
Apart from its role in stress response, APX2 is also involved in the regulation of plant growth and development. It participates in redox signaling pathways, which influence processes like seed germination, root architecture, leaf expansion, and flowering.
Yes, like many other genes, APX2 can have genetic variations, including single nucleotide polymorphisms (SNPs) and insertions/deletions (indels) in their coding or regulatory regions. These genetic polymorphisms can lead to variations in APX2 expression or activity, potentially influencing plant responses to stress or environmental conditions.
Yes, APX2 activity can be regulated by post-translational modifications such as phosphorylation and nitrosylation. These modifications can affect its enzymatic activity and stability, enabling fine-tuning of its antioxidant function.
Yes, APX2 expression can be regulated by various environmental factors. Factors such as light intensity, temperature, drought, salinity, pollutants, and heavy metals can influence APX2 expression levels in plants. These environmental cues often induce oxidative stress, and upregulating APX2 expression is an adaptive response to counteract the increased production of reactive oxygen species and maintain cellular redox homeostasis.
While APX2 itself has not been directly linked to specific plant diseases, it plays a vital role in plant defense against oxidative damage caused by various stresses. Oxidative stress, if left unchecked, can contribute to plant diseases. Therefore, APX2 indirectly contributes to plant disease resistance by protecting plant cells from oxidative damage.
The APX2 promoter region contains several known regulatory elements that can influence its transcriptional activity. These elements include stress-responsive elements (such as ABREs and WRKY binding sites), hormonal response elements (e.g., TGA boxes and EREs), light-responsive elements (e.g., G-Box and GT1-motifs), and various cis-acting elements related to developmental regulation and environmental cues. These elements allow APX2 expression to be finely tuned in response to different stimuli.
Yes, the expression of APX2 can be regulated by transcription factors. Several transcription factors have been identified that can directly bind to the promoter region of APX2 and modulate its transcriptional activity. For example, in Arabidopsis thaliana, transcription factors such as ZAT10, ABA-RESPONSIVE ELEMENT-BINDING FACTORs (ABFs), and WRKY33 have been shown to regulate APX2 expression in response to different stress signals.
Yes, researchers often create APX2 mutant or knockout plants to study the specific functions and mechanisms of APX2 in plant physiology. These mutants help elucidate the role of APX2 and its interactions with other plant components in stress tolerance and growth regulation.
Yes, APX2 expression or activity can serve as a biomarker for oxidative stress in plants. Its upregulation or downregulation in response to oxidative stressors can indicate the extent of cellular damage and the plant's ability to cope with oxidative stress. Therefore, monitoring APX2 expression or activity can provide valuable information about the oxidative status and stress tolerance of plants in various environmental conditions.
Yes, APX2 expression can be induced by phytohormones. Phytohormones such as abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) have been shown to upregulate APX2 expression in response to various stresses, including drought, pathogen attack, and oxidative stress. These hormonal signals play important roles in regulating APX2 levels and coordinating plant responses to environmental cues.
One challenge is the redundancy and functional overlap of multiple isoforms of ascorbate peroxidase in plants, including APX2. This makes it difficult to study the specific contributions of APX2 alone. Additionally, the complex regulatory networks that control APX2 expression and activity require further investigation to fully understand its role in plant physiology.
APX2 plays a crucial role in plant defense against pathogens by mitigating the oxidative burst associated with pathogen invasion. Pathogens often induce the generation of reactive oxygen species (ROS) as part of plant defense responses, and APX2 helps detoxify these ROS to prevent oxidative damage. Additionally, APX2 may indirectly modulate defense signaling pathways through redox-based signaling mechanisms, highlighting its importance in plant immune responses.
Apart from its peroxidase activity, APX2 has been reported to have non-enzymatic functions in certain contexts. It can act as a redox sensor or interact with other proteins to regulate their activity or stability. These non-enzymatic functions may contribute to the broader roles of APX2 in cellular signaling and stress responses.
Yes, plants typically possess multiple isoforms of ascorbate peroxidase, including APX1, APX3, and APX4. These isoforms may have distinct roles in different tissues or under specific stress conditions.
Customer Reviews (4)
Write a reviewAPX2 can be used in various experimental setups and assays, including in vitro cell culture studies, animal models, and even clinical investigations.
This versatility enables researchers to explore APX2's function and role in a range of biological processes, making it a valuable tool across diverse research fields.
One advantage of using APX2 protein in trials is its versatility.
In addition to the advantages of APX2 protein itself, the manufacturer can play a crucial role in supporting the research efforts.
Ask a Question for All apx2 Products
Required fields are marked with *
My Review for All apx2 Products
Required fields are marked with *