Matrix Protein Assays

Total collagen assay

This assay is to determine the total quantity of collagen precisely. It can be used to measure all forms of collagen (mature, immature, procollagen, degraded collagen, cross-linked collagen, collagen from various source). Our testing can cover a broad spectrum of samples including tissue extracts, cellular extracts, tissue culture supernatant and among others.

  • Quantitiative measurement of all types and all forms of collagen
  • Species independent
  • Broad dynamic range
  • Based on analysis of hydroxyproline

Hydroxyproline assay

This assay is used to determine the quantity of hydroxyproline. Hydroxyproline results from (acid) hydrolysis of collagen. Usually from this analysis special equipment is required for removal of the HCl. Our assay does not need this step.

  • Quantitative measurement of hydroxyproline
  • Can be applied to collagen analysis in all types of tissues

Soluble collagen assay

This assay detects soluble or (acid/pepsin) solubilized collagen in a variety of biological fluids and can be used for multiple animal species. It is intended for the use of conditioned cell culture medium and cellular extract samples. The assay is not affected by serum up to 10%.

  • Measures all types of fibrilar collagen
  • Species independent
  • Not affected by serum
  • Range: 0.5 to 10ug/ml. Sensitivity: 0.69ng/ml

Total protein assay

This assay is used for the quantitative determination of all types of protein in hydrolyzed samples. The assay is based on the formation of a blue colored dye from genipin with free amino acids. It measures the total amount of amino acids present in the hydrolyzed sample, with the exception of proline and hydroxyproline.

  • Quantitative measurement of protein in acid hydrolysates of fresh, frozen, fixed and paraffin embedded tissues
  • Species independent
  • Broad dynamic range
  • Range: 0.05 to 3.0 mg/ml. Sensitivity: 0.007mg/ml

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