Multiplex Protein Detection Assay

Creative BioMart’s Multiplex Protein Detection Assay provides an advanced solution for the simultaneous detection and quantification of multiple analytes, including proteins and peptides, within a single sample. With the capability of multiplexing up to 500 different assays at once, this service offers a high-throughput, cost-efficient approach to biomarker discovery and analysis. Combining powerful instrumentation, intuitive software, and meticulously validated assays, our platform ensures accurate, reproducible, and comprehensive results. By conserving sample volume and maximizing data output, Creative BioMart empowers researchers to accelerate discoveries across diverse biological pathways and complex experimental designs.

How Multiplex Protein Detection Assays Drive Breakthrough Discovery

The demand for efficient and reliable biomarker analysis has grown rapidly with the increasing complexity of biomedical research. Traditional single-analyte assays often require large sample volumes and extensive labor, limiting throughput and data quality. Multiplex protein detection technologies have emerged as a transformative solution, allowing researchers to measure multiple biomarkers in parallel while conserving precious biological material. This technology is particularly valuable for studies involving intricate biological processes such as metabolism, immune regulation, apoptosis, and extracellular matrix remodeling. Creative BioMart addresses this need with its high-performance multiplex protein detection assay, which combines precision, scalability, and user-friendly design.

Assay Multiplex Protein Detection Services

Creative BioMart’s multiplex protein detection assay is designed to provide both efficiency and accuracy in protein analysis. Its advanced features allow researchers to achieve reliable results while simplifying laboratory workflows.

Service Workflow

Service Details

Item	Details
Kit Format	1 x 96-well plate for medium- to high-throughput applications.
Core Technology	6.5 µm magnetic bead-based multiplexing.
Kit Components	Coupled magnetic beads, detection antibodies, assay and wash buffers, diluents, streptavidin-PE, flat-bottom plate, and sealing tape.
Compatibility	Designed for use with both vacuum and magnetic wash stations.
Customization	Assays can be tailored to specific research requirements, supporting diverse experimental designs.

Performance Advantages

• Premixed standards simplify setup and enable robust standard curves across broad dynamic ranges.
• Provides accurate and reproducible quantification of each target.
• Designed to reduce variability and enhance consistency across runs.

Broad Applications

The assay is versatile and applicable to a wide range of research areas, including:

• Metabolic biomarkers
• Chemokines
• Apoptosis regulators
• MMPs and TIMPs

Why Choose Our Multiplex Protein Detection Assay Services

High Multiplexing Capability: Analyze up to 500 proteins simultaneously, maximizing data yield from each precious sample.

Comprehensive Kit Design: All-in-one kits include every essential reagent and consumable for a seamless workflow.

Enhanced Reproducibility: Magnetic bead technology ensures consistent results with simplified plate processing.

Cost- and Sample-Efficient: Obtain robust data from minimal sample volumes, reducing experimental costs.

Broad Applicability: Suitable for diverse research areas including metabolism, immunology, cancer biology, and extracellular matrix studies.

Expert Support: Our scientific team provides dedicated guidance, customization options, and technical assistance to meet the needs of complex projects.

Real-World Applications and Case Studies

Case 1: Multiplex bead-based system for biomarker validation

Li et al., 2013. doi:10.1373/clinchem.2012.190983

This study presents a magnetic bead–based multiplex system designed to conserve precious clinical samples while enabling integrated protein and glycan analysis. Antibody-coupled beads were used to capture target proteins, with protein quantification achieved through antibody-based immunoassays and glycan profiling performed via lectin immunosorbent assays (LISAs). Tested on TIMP-1, tissue plasminogen activator, membrane metallo-endopeptidase, and DPP-4, the system matched the performance of conventional single assays while requiring minimal sample input. Applied to prostate cancer tissues, the approach revealed that fucosylated TIMP-1 and DPP-4 better distinguished aggressive from nonaggressive cases, highlighting its utility for biomarker discovery and validation.

Figure 1. Comparison of the multiplex immunoassays and single immunoassays for measurement of DPP-4, TIMP-1, tPA, and MME in serum. (Li et al., 2013)

Case 2: Magnetic vs. non-magnetic beads inmalaria serology

Ondigo et al., 2019. doi:10.7717/peerj.6120

This study compared non-magnetic and magnetic bead–based multiplex assays for measuring antibody responses to five Plasmodium falciparum vaccine candidates. Both bead types successfully coupled recombinant antigens and produced comparable mean fluorescence intensity (MFI) values across tested samples from western Kenya. While stored non-magnetic beads maintained performance, they showed greater aggregation. Magnetic beads, though more costly, offered higher recovery, easier handling, and faster protocol execution. Overall, magnetic beads proved to be a practical and efficient alternative to non-magnetic beads for malaria serology, supporting their use in high-throughput antibody assessment in vaccine studies.

Figure 2. Log MFI values obtained from individuals plasma samples generated using non-magnetic and magnetic beads in a 5-plex assay (n = 42). (Ondigo et al., 2019)

Customer Testimonials: What Our Clients Are Say

"We collaborated with Creative BioMart to evaluate biomarker panels for our metabolic disease program. Their multiplex protein detection assay allowed us to simultaneously analyze over 30 cytokines and chemokines from just 50 µL of patient plasma. The reproducibility of the results was excellent, and the premixed standards streamlined assay setup for our team. This platform has accelerated our biomarker discovery pipeline while saving significant time and resources."

— Director of Translational Research | Mid-Sized Pharmaceutical Company

"Our immunology group needed a reliable solution to assess multiple immune markers in preclinical mouse studies. Creative BioMart’s assay kits delivered precise, consistent data across hundreds of samples. The magnetic bead technology significantly reduced variability compared to our previous ELISA-based approach. Thanks to their support, we successfully profiled T-cell activation and apoptosis pathways in our checkpoint inhibitor project, leading to more confident go/no-go decisions."

— Principal Scientist, Immunology | Global Biotech Firm

“Our team needed a reliable solution to measure MMPs and TIMPs across hundreds of patient serum samples in a multicenter study. Creative BioMart’s multiplex protein detection assay delivered exactly that—robust, reproducible data with significantly reduced processing time. The all-in-one 96-well kit was intuitive, and the compatibility with our magnetic wash station streamlined workflow for our technicians. Most importantly, we obtained high-quality data from minimal sample volumes, preserving precious patient material.”

— Director of Immunology R&D | Global Pharmaceutical Company

"Our cancer research team required multiplex assays to study tumor microenvironment dynamics. Creative BioMart provided a tailored panel for apoptosis and chemokine markers, and the results revealed insights we could not have obtained otherwise. The technical support team helped optimize our workflow. Their assay became a cornerstone of our publication that detailed chemokine-driven immune suppression in solid tumors."

— R&D Director, Oncology | Academic Research Institute

FAQs: Quick Answers About Our Multiplex Protein Detection Assays

• Q: What is the main advantage of using a multiplex protein detection assay over traditional single-analyte assays?

  A: Multiplex assays allow simultaneous detection of up to 500 different proteins or peptides from a single, small-volume sample. This not only conserves precious specimens but also reduces costs and significantly increases throughput compared to running multiple ELISAs.

• Q: What sample volumes are required for your multiplex assays?

  A: Our kits are designed for minimal sample input. In most cases, only 25–50 µL of serum, plasma, saliva, or other biological fluid is needed, making them ideal for studies with limited or valuable specimens.

• Q: How reliable and reproducible are your results?

  A: Built on 6.5 µm magnetic bead technology, our platform simplifies washing, minimizes variability, and enhances reproducibility. With optimized workflows and statistical adjustment options, we ensure consistent results across batches and experiments.

• Q: What types of biomarkers can be analyzed with your service?

  A: Our assay panels cover a broad spectrum of biological pathways, including metabolic markers, chemokines, apoptosis regulators, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs). Custom panels can also be developed to meet project-specific needs.

• Q: Is the workflow complicated to adopt in a busy lab setting?

  A: Not at all. Our all-in-one, space-saving kit includes all required reagents—magnetic beads, buffers, antibodies, standards, and consumables. Combined with intuitive data analysis software, the workflow is highly user-friendly and easily integrated into existing laboratory routines.

• Q: How do multiplex assays compare in terms of cost-effectiveness?

  A: By consolidating dozens of single assays into a single multiplex run, clients typically save 50–60% on consumables and cut hands-on time by more than half, without compromising data quality.

• Q: Can assays be customized for my research project?

  A: Yes. Creative BioMart offers flexible customization services, from tailored biomarker panels to adapted workflows, ensuring the assay aligns precisely with your research goals and study design.

References:

1. Li D, Chiu H, Chen J, Zhang H, Chan DW. Integrated analyses of proteins and their glycans in a magnetic bead–based multiplex assay format. Clinical Chemistry. 2013;59(1):315-324. doi:10.1373/clinchem.2012.190983
2. Ondigo BN, Park GS, Ayieko C, Nyangahu DD, Wasswa R, John CC. Comparison of non-magnetic and magnetic beads multiplex assay for assessment of Plasmodium falciparum antibodies. PeerJ. 2019;7:e6120. doi:10.7717/peerj.6120