Nanodisc Technology Platform

Nanodiscs are discoidal lipid bilayers 8-16 nm in diameter stabilized and solubilized in aqueous solution by two encircling amphipathic helical protein ribbons (called membrane scaffolding proteins).

The size of the Nanodisc is determined by the length of the membrane scaffolding proteins and the ratio of lipids used in the self-assembly process. The resulting disk-like bilayers can be homogeneous, monodisperse, and highly productive.

Most membrane proteins can maintain stability and activity in buffers containing detergents. However, a few membrane proteins sensitive to detergents are unsuitable for buffer solutions containing detergents. They will display an inability to be purified, poor stability, and inactivity.

Our Nanodisc Technology Platform solves the problem of protein sensitivity to detergents. Depending on the stabilizer, Nanodiscs can be classified into different types: Membrane scaffold proteins (MSPs) Nanodisc and Synthetic Nanodisc.

MSP Nanodisc uses membrane scaffold proteins (MSPs) as stabilizers to encapsulate artificial phospholipid components and transmembrane proteins to form a Nanodisc. The size of the Nanodisc is determined by the use of MSPs.

Synthetic Nanodisc is a disc structure with a diameter around 10nm that can keep transmembrane proteins in the environment of lipid bilayer, maintaining their conformation and biological function. Synthetic Nanodiscs use synthetic polymers to dissolve cell membranes and form Nanodisc structures around membrane proteins using native cellular phospholipids. The polymers act as both solubilizers and stabilizers, without the need for additional detergents.

Figure 1. Schematic diagram of the Nanodisc preparation process

Advantages

Nanodisc technology provides higher stability and reproducibility, which helps to obtain accurate and reliable experimental results. The Nanodisc technology platform has wide applicability in various biological research fields. Not only is it used to study the structure, function, interaction, and signaling of membrane proteins, but also can be used to study the structure of membrane proteins and drug-drug interactions during drug screening and development.

Creative Biomart is committed to providing high-quality services to our customers. Our Nanodisc technology platform ensures the activity and stability of membrane proteins for your subsequent assay experiments.

Creative Biomart provides customized membrane protein expression purification services according to customer needs, designing and tuning the protocols according to the nature of the proteins and your experimental requirements, ensuring quality results. If you have any questions, please do not hesitate to contact us!