What is NIPBL Protein
The NIPBL protein, an intricate player in cellular orchestration, is encoded by the NIPBL gene.
What is NIPBL Protein?
The NIPBL protein, an abbreviation for Nipped-B-like, is a linchpin in embryonic development. The HEAT repeat, a prominent structural motif within NIPBL, facilitates intricate protein-protein interactions. Understanding NIPBL's structure is pivotal for unraveling its functional intricacies.
Figure 1. Overall structure of the human cohesin-NIPBLC-DNA complex. (Shi, Z., et al. 2020)
The Function of NIPBL Protein
The primary function of the NIPBL protein is to regulate the cohesin complex, a group of proteins responsible for holding sister chromatids together. This cohesion is crucial during cell division, ensuring the accurate distribution of genetic material to daughter cells.
NIPBL accomplishes this by loading the cohesin complex onto chromosomes during a specific phase of the cell cycle, known as the G2 phase. Proper regulation of the cohesin complex is essential for maintaining genomic stability and preventing errors in chromosome segregation.
NIPBL-Related Diseases
Disruptions in the NIPBL gene or abnormalities in the NIPBL protein can lead to a group of developmental disorders known as cohesinopathies. Cornelia de Lange Syndrome (CdLS) is one such disorder associated with mutations in the NIPBL gene. CdLS is characterized by facial dysmorphisms, growth retardation, intellectual disabilities, and limb abnormalities.
Understanding the link between NIPBL and cohesinopathies provides crucial insights into the molecular mechanisms underlying these disorders. Research efforts are focused on deciphering how NIPBL mutations contribute to the manifestation of cohesinopathies, with the aim of developing targeted therapeutic interventions.
NIPBL Related Signaling Pathways
The intricate web of signal pathways within cells governs various biological processes. The NIPBL protein is no exception, being part of a signaling cascade that ensures the proper functioning of the cohesin complex.
One notable pathway involving NIPBL is the Wnt signaling pathway. Wnt signaling plays a crucial role in embryonic development, tissue regeneration, and maintaining adult stem cells. NIPBL has been identified as a critical player in modulating Wnt signaling, highlighting its significance beyond the realm of chromosome cohesion.
Elucidating the crosstalk between NIPBL and Wnt signaling opens new avenues for understanding the broader implications of NIPBL in cellular processes. This knowledge may contribute to the development of therapeutic strategies targeting these pathways in diseases where their dysregulation is implicated.
Applications of NIPBL in Biomedical Research
- Cancer Research
NIPBL's role in chromosomal stability positions it as a focal point in cancer research. Dysregulation of chromosomal cohesion is rampant in cancer cells, making NIPBL a potential target for innovative therapeutic strategies.
- Regenerative Medicine
The connection between NIPBL and Wnt signaling introduces avenues for regenerative medicine. Exploring how NIPBL influences stem cells and tissue regeneration could revolutionize approaches to tissue repair and rejuvenation.
- Therapeutic Target for Cohesinopathies
NIPBL mutations contribute to cohesinopathies, making it a prime therapeutic target. Interventions, ranging from gene therapy to pharmacological approaches, aim to rectify NIPBL function, mitigating the symptoms associated with these disorders.
- Drug Development
NIPBL's involvement in Wnt signaling positions it as a potential target for drug development. Modulating this pathway through NIPBL could have profound implications in diseases where Wnt signaling is dysregulated, such as certain cancers and degenerative disorders.
In the complex tapestry of molecular biology, NIPBL emerges as a pivotal player with implications far beyond chromosomal cohesion. From its structural nuances to its multifaceted functions and promising applications in biomedicine, NIPBL captivates researchers seeking to unlock its full potential. As investigations continue, the horizon of possibilities widens, offering new avenues for therapeutic interventions and advancing our understanding of cellular dynamics.
Recommended Products for NIPBL Protein
| Cat.# | Species | Product name | Source (Host) | Tag |
|---|---|---|---|---|
| NIPBL-1296H | Human | Recombinant Human NIPBL, GST-tagged | E.coli | GST |
| NIPBL-6635HF | Human | Recombinant Full Length Human NIPBL Protein, GST-tagged | In Vitro Cell Free System | GST |
| NIPBL-5872H | Human | Recombinant Human NIPBL Protein, GST-tagged | Wheat Germ | GST |
| NIPBL-10676M | Mouse | Recombinant Mouse NIPBL Protein | Mammalian Cell | His |
| NIPBL-6073M | Mouse | Recombinant Mouse NIPBL Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi |
Reference
- Shi, Z., et al. Cryo-EM structure of the human cohesin-NIPBL-DNA complex. Science. 2020, 368(6498): 1454-1459.
