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ACAT1

  • Official Full Name

    acetyl-CoA acetyltransferase 1

  • Overview

    This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
  • Synonyms

    ACAT1; acetyl-CoA acetyltransferase 1; ACAT, acetyl Coenzyme A acetyltransferase 1; acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl Coenzyme A thiolase; THIL; acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoac;

  • Recombinant Proteins
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Cynomolgus Monkey
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • Zebrafish
  • E.coli
  • E.Coli or Yeast
  • HEK293
  • In Vitro Cell Free System
  • Mammalian Cell
  • GST
  • His
  • His (Fc)
  • Avi
  • His|T7
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human ACAT1-3045H Recombinant Human ACAT1, His-tagged E.coli His 427
Human ACAT1-914HF Recombinant Full Length Human ACAT1 Protein, GST-tagged In Vitro Cell Free System GST 162 amino acids
Human ACAT1-2475H Recombinant Human ACAT1 protein E.coli N/A 34-427aa
Human ACAT1-0654H Recombinant Human ACAT1 Protein (Ala111-Leu427), N-His-tagged E.coli N-His Ala111-Leu427
Human ACAT1-913HF Recombinant Full Length Human ACAT1 Protein, GST-tagged In Vitro Cell Free System GST 162 amino acids
Human ACAT1-01H Active Recombinant Human ACAT1 Protein, His-tagged E.coli His
Mouse Acat1-8159M Recombinant Mouse Acat1 protein, His & T7-tagged E.coli His/T7 Ala31~Leu424 (Accession # Q8QZT1)
Rat Acat1-3050R Recombinant Rat Acat1, His-tagged E.Coli or Yeast His 424
Cynomolgus Monkey ACAT1-265C Recombinant Cynomolgus ACAT1 Protein, His-tagged Mammalian Cell His
Cynomolgus Monkey ACAT1-15C-B Recombinant Cynomolgus Monkey ACAT1 Protein Pre-coupled Magnetic Beads HEK293
Cynomolgus Monkey ACAT1-15C Recombinant Cynomolgus Monkey ACAT1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque ACAT1-201R Recombinant Rhesus monkey ACAT1 Protein, His-tagged Mammalian Cell His
Rhesus Macaque ACAT1-30R-B Recombinant Rhesus Macaque ACAT1 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque ACAT1-30R Recombinant Rhesus Macaque ACAT1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Zebrafish ACAT1-1085Z Recombinant Zebrafish ACAT1 Mammalian Cell His
Chicken ACAT1-5141C Recombinant Chicken ACAT1 Mammalian Cell His
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • ACAT1 Related Articles

ACAT1 involved in several pathways and played different roles in them. We selected most pathways ACAT1 participated on our site, such as Fatty acid degradation, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation, which may be useful for your reference. Also, other proteins which involved in the same pathway with ACAT1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Fatty acid degradationADH5;ADH1A;GCDHA;HADHA;CPT1C;ADH4;EHHADH;HADH;GCDHB
Synthesis and degradation of ketone bodiesHMGCL;OXCT2A;OXCT1A;ACAT1;BDH2;OXCT1B;OXCT1;OXCT2B;OXCT2
Valine, leucine and isoleucine degradationALDH2;IL4I1;HADHB;HADHAB;BCKDHA;ALDH2.1;MCCC1;HMGCS1;AOX3
Lysine degradationHYKK;EHMT2;ALDH9A1A.1;KMT2A;DLST;ACAT2;ALDH3A2A;OGDHL;C2orf34
Tryptophan metabolismAANAT2;ANI_1_2390104;ALDH2.2;ACAT1;MAOA;IDO1;RNF25;CMTM2A;AOX2P
Pyruvate metabolismPKMB;GRHPRB;LDHB;MPC1;PDHA1A;FH1;PDPR;PDP2;ACSS2L
Glyoxylate and dicarboxylate metabolismAMT;SHMT1;HAO2;GLYCTK;CAT;GLULB;MUT;GRHPRA;SHMT2
Propanoate metabolismLDHAL6B;HADHAB;PCCA;SUCLG2;ACADM;ECHDC1;SUCLA2;ALDH6A1;ackA
Butanoate metabolismGAD1B;HADHAA;ACAT2;HADHAB;BDH2;ACSM4;ECHS1;OXCT1B;ACSM2B
Terpenoid backbone biosynthesisFDPS;MVDA;ACAT1;PDSS2;GGPS1;IDI2;IDI1;PCYOX1;ICMT
Metabolic pathwaysCMAS;LPCAT4;RPIA;SAT2B;ATP5G1;ST8SIA5;ATP6V1G3;HSD17B12;NAT
Carbon metabolismFBP1;IDH3A;PKLR;PDHA2;AMT;ACAT2;SDHA;PCXB;GAPDH
Fatty acid metabolismACAT1;HADHB;HSD17B12;ACACA;ACADS;OXSM;ACSBG1;CPT1A;ACSL3A

ACAT1 has several biochemical functions, for example, acetyl-CoA C-acetyltransferase activity, coenzyme binding, enzyme binding. Some of the functions are cooperated with other proteins, some of the functions could acted by ACAT1 itself. We selected most functions ACAT1 had, and list some proteins which have the same functions with ACAT1. You can find most of the proteins on our site.

Function Related Protein
acetyl-CoA C-acetyltransferase activityACAT1;ACAT2;ACAA1A;HADHA
coenzyme bindingWWOX;ATAT1;GCLC;QDPRB1;TDH;NDUFA9A;TSTA3;ACAT1;TDH2
enzyme bindingPIAS3;RARA;SPDL1;YES1;NOP14;HIST1H2AG;CASP2;USF1;HAND1
metal ion bindingFUZ;IKZF3;ZNF23;MTMR4;ZSCAN4;ELMSAN1A;ZNF70;PPTC7;ZNF45L
protein homodimerization activityBHLHA15;NUDT21;TERF2;CHMP4C;XPA;CTSE;WHRN;P2RX4;BST2

ACAT1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with ACAT1 here. Most of them are supplied by our site. Hope this information will be useful for your research of ACAT1.

VCAM1; EIF1B; GH1; MCC; MPG; HLA-B; HLA-C; TNIK; AGXT; GABARAPL2; SIRT4

Chang, HT; Olson, LK; et al. Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy. NUTRITION & METABOLISM 10:-(2013).
Yang, Y; Wang, YF; et al. Specific Kv1.3 blockade modulates key cholesterol-metabolism-associated molecules in human macrophages exposed to ox-LDL. JOURNAL OF LIPID RESEARCH 54:34-43(2013).
  • Q&As
  • Reviews

Q&As (10)

Ask a question
I want to buy ACAT1 protein, is there any discount? 09/16/2022

Creative BioMart provides coupons for each new customer and runs promotions on holidays. For specific promotions, please contact our sales staff for more information.

What is the role of ACAT1 in NAFLD? 09/15/2022

Studies have demonstrated that upregulation of ACAT1 expression and activity can lead to the development of hepatic steatosis, a hallmark of NAFLD. Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD.

Can I get a discount on the price of recombinant ACAT1 protein? 12/12/2021

Coupons are available for every new customer, and we run promotions on important holidays. For specific promotions, please contact our sales staff for more information.

What is the significance of ACAT1 in lipid metabolism? 08/10/2021

ACAT1 is a key enzyme involved in the biosynthesis of cholesterol and ketone bodies, as well as in the breakdown of fatty acids through beta-oxidation. It plays a critical role in maintaining lipid homeostasis in the liver and regulating the levels of circulating cholesterol and fatty acids.

How is ACAT1 regulated in the liver? 06/18/2021

ACAT1 expression is regulated by a variety of factors, including hormonal signals such as insulin and glucagon, as well as by the levels of intracellular metabolites such as acetyl-CoA and cholesterol. In addition, ACAT1 activity is subject to post-translational modifications such as phosphorylation, which can modulate its enzymatic activity.

What is the structural basis for ACAT1 function? 05/08/2021

ACAT1 is a homotetrameric protein that consists of four identical subunits. Each subunit contains a catalytic domain that is responsible for the transfer of acetyl-CoA, as well as a regulatory domain that modulates enzymatic activity. The crystal structure of ACAT1 has been solved, revealing key structural features such as the location of the active site and the binding sites for acetyl-CoA and cholesterol.

Can recombinant ACAT1 protein be used as a diagnostic or therapeutic agent? 09/18/2020

Our recombinant ACAT1 protein is primarily used as a research tool and is not approved for diagnostic or therapeutic purposes.

What are the potential therapeutic applications of ACAT1 inhibition? 05/01/2020

Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD, as well as other metabolic disorders such as hypercholesterolemia and atherosclerosis.

How can the recombinant ACAT1 protein be used in research or applications? 02/01/2020

The recombinant ACAT1 protein can be used in a variety of research and application settings, such as studying the biochemistry and enzymology of the ACAT1 enzyme, screening for inhibitors or activators of the enzyme, or developing diagnostic assays for diseases related to cholesterol metabolism. The protein can also be used as a reference standard or control in analytical methods such as mass spectrometry or ELISA. However, it is important to note that the specific use of the recombinant ACAT1 protein will depend on the goals and requirements of the individual research or application.

How is the recombinant ACAT1 protein quantified and characterized? 01/16/2020

Recombinant protein is typically quantified using a combination of techniques, such as UV spectrophotometry, Bradford protein assay, or bicinchoninic acid (BCA) assay. To characterize the protein, a variety of methods can be used, including SDS-PAGE, Western blotting, mass spectrometry, and enzyme activity assays.

Customer Reviews (3)

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Reviews
01/16/2023

    The recombinant human ACAT1 protein was affordable and highly effective.

    09/16/2022

      The customization services provided by Creative BioMart's technical support team for the recombinant ACAT1 protein were invaluable. I am grateful for their expertise and professionalism.

      10/07/2020

        I appreciate the level of expertise and professionalism demonstrated by Creative BioMart's scientists. Their Recombinant Human ACAT1 Protein helped me achieve the results I needed for my project.

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