ACAT1
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Official Full Name
acetyl-CoA acetyltransferase 1
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Overview
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. -
Synonyms
ACAT1; acetyl-CoA acetyltransferase 1; ACAT, acetyl Coenzyme A acetyltransferase 1; acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl Coenzyme A thiolase; THIL; acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoac;
- Recombinant Proteins
- Protein Pre-coupled Magnetic Beads
- Chicken
- Cynomolgus Monkey
- Human
- Mouse
- Rat
- Rhesus Macaque
- Zebrafish
- E.coli
- E.Coli or Yeast
- HEK293
- In Vitro Cell Free System
- Mammalian Cell
- GST
- His
- His (Fc)
- Avi
- His|T7
- N/A
- N
Species | Cat.# | Product name | Source (Host) | Tag | Protein Length | Price |
---|---|---|---|---|---|---|
Human | ACAT1-3045H | Recombinant Human ACAT1, His-tagged | E.coli | His | 427 | |
Human | ACAT1-914HF | Recombinant Full Length Human ACAT1 Protein, GST-tagged | In Vitro Cell Free System | GST | 162 amino acids | |
Human | ACAT1-2475H | Recombinant Human ACAT1 protein | E.coli | N/A | 34-427aa | |
Human | ACAT1-0654H | Recombinant Human ACAT1 Protein (Ala111-Leu427), N-His-tagged | E.coli | N-His | Ala111-Leu427 | |
Human | ACAT1-913HF | Recombinant Full Length Human ACAT1 Protein, GST-tagged | In Vitro Cell Free System | GST | 162 amino acids | |
Human | ACAT1-01H | Active Recombinant Human ACAT1 Protein, His-tagged | E.coli | His | ||
Mouse | Acat1-8159M | Recombinant Mouse Acat1 protein, His & T7-tagged | E.coli | His/T7 | Ala31~Leu424 (Accession # Q8QZT1) | |
Rat | Acat1-3050R | Recombinant Rat Acat1, His-tagged | E.Coli or Yeast | His | 424 | |
Cynomolgus Monkey | ACAT1-265C | Recombinant Cynomolgus ACAT1 Protein, His-tagged | Mammalian Cell | His | ||
Cynomolgus Monkey | ACAT1-15C-B | Recombinant Cynomolgus Monkey ACAT1 Protein Pre-coupled Magnetic Beads | HEK293 | |||
Cynomolgus Monkey | ACAT1-15C | Recombinant Cynomolgus Monkey ACAT1 Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi | ||
Rhesus Macaque | ACAT1-201R | Recombinant Rhesus monkey ACAT1 Protein, His-tagged | Mammalian Cell | His | ||
Rhesus Macaque | ACAT1-30R-B | Recombinant Rhesus Macaque ACAT1 Protein Pre-coupled Magnetic Beads | HEK293 | |||
Rhesus Macaque | ACAT1-30R | Recombinant Rhesus Macaque ACAT1 Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi | ||
Zebrafish | ACAT1-1085Z | Recombinant Zebrafish ACAT1 | Mammalian Cell | His | ||
Chicken | ACAT1-5141C | Recombinant Chicken ACAT1 | Mammalian Cell | His |
- Involved Pathway
- Protein Function
- Interacting Protein
- ACAT1 Related Articles
ACAT1 involved in several pathways and played different roles in them. We selected most pathways ACAT1 participated on our site, such as Fatty acid degradation, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation, which may be useful for your reference. Also, other proteins which involved in the same pathway with ACAT1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
Pathway Name | Pathway Related Protein |
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Fatty acid degradation | ADH5;ADH1A;GCDHA;HADHA;CPT1C;ADH4;EHHADH;HADH;GCDHB |
Synthesis and degradation of ketone bodies | HMGCL;OXCT2A;OXCT1A;ACAT1;BDH2;OXCT1B;OXCT1;OXCT2B;OXCT2 |
Valine, leucine and isoleucine degradation | ALDH2;IL4I1;HADHB;HADHAB;BCKDHA;ALDH2.1;MCCC1;HMGCS1;AOX3 |
Lysine degradation | HYKK;EHMT2;ALDH9A1A.1;KMT2A;DLST;ACAT2;ALDH3A2A;OGDHL;C2orf34 |
Tryptophan metabolism | AANAT2;ANI_1_2390104;ALDH2.2;ACAT1;MAOA;IDO1;RNF25;CMTM2A;AOX2P |
Pyruvate metabolism | PKMB;GRHPRB;LDHB;MPC1;PDHA1A;FH1;PDPR;PDP2;ACSS2L |
Glyoxylate and dicarboxylate metabolism | AMT;SHMT1;HAO2;GLYCTK;CAT;GLULB;MUT;GRHPRA;SHMT2 |
Propanoate metabolism | LDHAL6B;HADHAB;PCCA;SUCLG2;ACADM;ECHDC1;SUCLA2;ALDH6A1;ackA |
Butanoate metabolism | GAD1B;HADHAA;ACAT2;HADHAB;BDH2;ACSM4;ECHS1;OXCT1B;ACSM2B |
Terpenoid backbone biosynthesis | FDPS;MVDA;ACAT1;PDSS2;GGPS1;IDI2;IDI1;PCYOX1;ICMT |
Metabolic pathways | CMAS;LPCAT4;RPIA;SAT2B;ATP5G1;ST8SIA5;ATP6V1G3;HSD17B12;NAT |
Carbon metabolism | FBP1;IDH3A;PKLR;PDHA2;AMT;ACAT2;SDHA;PCXB;GAPDH |
Fatty acid metabolism | ACAT1;HADHB;HSD17B12;ACACA;ACADS;OXSM;ACSBG1;CPT1A;ACSL3A |
ACAT1 has several biochemical functions, for example, acetyl-CoA C-acetyltransferase activity, coenzyme binding, enzyme binding. Some of the functions are cooperated with other proteins, some of the functions could acted by ACAT1 itself. We selected most functions ACAT1 had, and list some proteins which have the same functions with ACAT1. You can find most of the proteins on our site.
Function | Related Protein |
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acetyl-CoA C-acetyltransferase activity | ACAT1;ACAT2;ACAA1A;HADHA |
coenzyme binding | WWOX;ATAT1;GCLC;QDPRB1;TDH;NDUFA9A;TSTA3;ACAT1;TDH2 |
enzyme binding | PIAS3;RARA;SPDL1;YES1;NOP14;HIST1H2AG;CASP2;USF1;HAND1 |
metal ion binding | FUZ;IKZF3;ZNF23;MTMR4;ZSCAN4;ELMSAN1A;ZNF70;PPTC7;ZNF45L |
protein homodimerization activity | BHLHA15;NUDT21;TERF2;CHMP4C;XPA;CTSE;WHRN;P2RX4;BST2 |
ACAT1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with ACAT1 here. Most of them are supplied by our site. Hope this information will be useful for your research of ACAT1.
VCAM1; EIF1B; GH1; MCC; MPG; HLA-B; HLA-C; TNIK; AGXT; GABARAPL2; SIRT4
- Q&As
- Reviews
Q&As (10)
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Studies have demonstrated that upregulation of ACAT1 expression and activity can lead to the development of hepatic steatosis, a hallmark of NAFLD. Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD.
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ACAT1 is a key enzyme involved in the biosynthesis of cholesterol and ketone bodies, as well as in the breakdown of fatty acids through beta-oxidation. It plays a critical role in maintaining lipid homeostasis in the liver and regulating the levels of circulating cholesterol and fatty acids.
ACAT1 expression is regulated by a variety of factors, including hormonal signals such as insulin and glucagon, as well as by the levels of intracellular metabolites such as acetyl-CoA and cholesterol. In addition, ACAT1 activity is subject to post-translational modifications such as phosphorylation, which can modulate its enzymatic activity.
ACAT1 is a homotetrameric protein that consists of four identical subunits. Each subunit contains a catalytic domain that is responsible for the transfer of acetyl-CoA, as well as a regulatory domain that modulates enzymatic activity. The crystal structure of ACAT1 has been solved, revealing key structural features such as the location of the active site and the binding sites for acetyl-CoA and cholesterol.
Our recombinant ACAT1 protein is primarily used as a research tool and is not approved for diagnostic or therapeutic purposes.
Inhibiting ACAT1 has been proposed as a potential therapeutic approach for treating NAFLD, as well as other metabolic disorders such as hypercholesterolemia and atherosclerosis.
The recombinant ACAT1 protein can be used in a variety of research and application settings, such as studying the biochemistry and enzymology of the ACAT1 enzyme, screening for inhibitors or activators of the enzyme, or developing diagnostic assays for diseases related to cholesterol metabolism. The protein can also be used as a reference standard or control in analytical methods such as mass spectrometry or ELISA. However, it is important to note that the specific use of the recombinant ACAT1 protein will depend on the goals and requirements of the individual research or application.
Recombinant protein is typically quantified using a combination of techniques, such as UV spectrophotometry, Bradford protein assay, or bicinchoninic acid (BCA) assay. To characterize the protein, a variety of methods can be used, including SDS-PAGE, Western blotting, mass spectrometry, and enzyme activity assays.
Customer Reviews (3)
Write a reviewThe recombinant human ACAT1 protein was affordable and highly effective.
The customization services provided by Creative BioMart's technical support team for the recombinant ACAT1 protein were invaluable. I am grateful for their expertise and professionalism.
I appreciate the level of expertise and professionalism demonstrated by Creative BioMart's scientists. Their Recombinant Human ACAT1 Protein helped me achieve the results I needed for my project.
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