Active Recombinant Human ACAT1 Protein, His-tagged

• Cat.No.: ACAT1-01H
• Product Overview: E. coli-derived human ACAT1 protein
  Val34-Leu427 with an N-terminal Met and 6-His tag.

Specification

• Description: This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
• Source: Escherichia coli
• Species: Human
• Tag: His
• Bio-activity: Measured by its ability to convert acetoacetyl-CoA and CoA into acetyl-CoA. The specific activity is >15000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: 42 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 °C as supplied.
  3 months, -20 to -70 °C under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and TCEP.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: ACAT1 acetyl-CoA acetyltransferase 1 [ Homo sapiens (human) ]
• Official Symbol: ACAT1
• Synonyms: ACAT; ACAT1; acetoacetyl Coenzyme A thiolase; Acetoacetyl-CoA thiolase; acetyl-CoA acetyltransferase 1; acetyl-CoA acetyltransferase, mitochondrial; acetyl-Coenzyme A acetyltransferase 1; EC 2.3.1; EC 2.3.1.9; MAT; mitochondrial acetoacetyl-CoA thiolase; T2; THIL
• Gene ID: 38
• mRNA Refseq: NM_000019.4
• Protein Refseq: NP_000010.1
• MIM: 607809
• UniProt ID: P24752