Recombinant Human ACAT1, His-tagged

• Cat.No.: ACAT1-3045H
• Product Overview: ACAT1

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 427
• Description: This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
• Form: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 43.8kDa (417aa) confirmed by MALDI-TOF
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Notes: Small volumes of ACAT1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Warning: This product is for research use only. Not for use in diagnostic or therapeutic procedures.

Gene Information

• Gene Name: ACAT1 acetyl-CoA acetyltransferase 1 [ Homo sapiens ]
• Official Symbol: ACAT1
• Synonyms: ACAT1; acetyl-CoA acetyltransferase 1; ACAT, acetyl Coenzyme A acetyltransferase 1; acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl Coenzyme A thiolase; THIL; acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; T2; MAT; ACAT
• Gene ID: 38
• mRNA Refseq: NM_000019
• Protein Refseq: NP_000010
• MIM: 607809
• UniProt ID: P24752
• Chromosome Location: 11q22.3
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; C5 isoprenoid biosynthesis, mevalonate pathway, organism-specific biosystem; C5 isoprenoid bio
• Function: acetyl-CoA C-acetyltransferase activity; coenzyme binding; enzyme binding; metal ion binding; protein homodimerization activity; transferase activity, transferring acyl groups other than amino-acyl groups