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TRAF2

  • Official Full Name

    TNF receptor-associated factor 2

  • Overview

    TNF receptor associated factor 2 (TRAF2) is thought to be a common signal transducer that associates with the cytoplasmic domains of the tumor necrosis factor receptor (TNFR) superfamily such as TNFR2, CD40 and CD30. TRAF2 indirectly associates with TNFR1
  • Synonyms

    TRAF2; TNF receptor-associated factor 2; TRAP3; E3 ubiquitin-protein ligase TRAF2; tumor necrosis factor type 2 receptor associated protein 3; tumor necrosis factor type 2 receptor-associated protein 3; TRAP; MGC:45012;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Mouse
  • Rhesus Macaque
  • E. coli
  • HEK293
  • HEK293T
  • Insect cell
  • Mammalian Cell
  • Mammalian cells
  • Flag
  • GST
  • His
  • Fc
  • Avi
  • Myc
  • DDK
  • Non
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human TRAF2-1500H Recombinant Human TRAF2 Protein, GST-tagged Insect cell GST
Human TRAF2-31601TH Recombinant Human TRAF2, His-tagged His
Human TRAF2-824HCL Recombinant Human TRAF2 293 Cell Lysate HEK293 Non
Human TRAF2-2248H-B Recombinant Human TRAF2 Protein Pre-coupled Magnetic Beads HEK293
Human TRAF2-1764HFL Recombinant Full Length Human TRAF2 Protein, C-Flag-tagged Mammalian cells Flag
Human TRAF2-2248H Recombinant Human TRAF2 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Human TRAF2-01H Recombinant Full Length Human TRAF2 Protein, His tagged E. coli His 1-501 aa
Mouse TRAF2-17284M Recombinant Mouse TRAF2 Protein Mammalian Cell His
Mouse TRAF2-9556M-B Recombinant Mouse TRAF2 Protein Pre-coupled Magnetic Beads HEK293
Mouse TRAF2-9556M Recombinant Mouse TRAF2 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Mouse Traf2-6617M Recombinant Mouse Traf2 Protein, Myc/DDK-tagged HEK293T Myc&DDK
Rhesus Macaque TRAF2-4931R Recombinant Rhesus monkey TRAF2 Protein, His-tagged Mammalian Cell His
Rhesus Macaque TRAF2-4745R Recombinant Rhesus Macaque TRAF2 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Rhesus Macaque TRAF2-4745R-B Recombinant Rhesus Macaque TRAF2 Protein Pre-coupled Magnetic Beads HEK293
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
  • TRAF2 Related Signal Pathway

What is TRAF2 protein?

TRAF2 (TNF receptor associated factor 2) gene is a protein coding gene which situated on the long arm of chromosome 9 at locus 9q34. The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. The TRAF2 protein is consisted of 501 amino acids and its molecular mass is approximately 55.9 kDa.

What is the function of TRAF2 protein?

TRAF2 is an intracellular signaling protein that is mainly involved in the signaling process of a variety of cell surface receptors, especially in the signaling of members of the tumor necrosis factor receptor (TNFR) family. By binding to these receptors, TRAF2 is involved in the regulation of a variety of cellular responses, including cell survival, proliferation, differentiation, inflammatory response, and immune response. The TRAF2 protein's function is not limited to signaling, it is also involved in regulating the stability and activity of other signaling molecules in the cell, Such as NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and JNK (c-Jun N-terminal kinases), It affects how cells respond to various physiological and pathological stimuli.

TRAF2 Related Signaling Pathway

TRAF2 proteins are involved in a variety of signaling pathways, the most important of which are the tumor necrosis factor (TNF) receptor superfamily and the nuclear factor κB (NF-κB) signaling pathway. As a member of the tumor necrosis factor receptor-related factor family, TRAF2 is involved in regulating biological processes such as inflammation, immune response, and apoptosis by binding to TNF receptors. In addition, TRAF2 interacts with other signaling pathways, such as Toll-like receptors (TLR), NOd-like receptors (NLR), and RIP1/RIP3 complexes, which further affect cell proliferation, differentiation, and survival.

TRAF2 Related Diseases

TRAF2 protein is associated with a variety of diseases, including autoimmune diseases, tumors, and infectious diseases. In autoimmune diseases, abnormal expression or functional mutation of TRAF2 may lead to overactivation of the immune system, triggering diseases such as rheumatoid arthritis, systemic lupus erythematosus, scleroderma, etc. In addition, TRAF2 is also involved in cell proliferation, differentiation and apoptosis, and is closely related to the occurrence and development of tumors. Studies have shown that TRAF2 expression levels are abnormally elevated in various tumors such as lung cancer, breast cancer, and colon cancer. At the same time, TRAF2 is also involved in the antiviral immune response, and its abnormal function may lead to the persistence and chronicity of viral infection.

A model for TRAF2 in liver tumorigenesis.jpg

Fig1. A model for TRAF2 in liver tumorigenesis. (Xue Liang, 2023)

Bioapplications of TRAF2

In the field of drug discovery, TRAF2 has become an important drug target due to its key role in a variety of diseases. By inhibiting or activating the TRAF2 signaling pathway, researchers and pharmaceutical companies are developing new treatments to regulate the inflammatory response and tumor development. In terms of clinical diagnosis, TRAF2 expression levels are significantly elevated in some inflammatory diseases as well as certain tumors, making it a potential biomarker for these diseases.

High Purity

SDS-PAGE (TRAF2-1764HFL).jpg

Fig1. SDS-PAGE (TRAF2-1764HFL)

Case Study 1: Yawei Xu, 2023

The management of advanced clear cell renal cell carcinoma (ccRCC) remains a major challenge in clinical practice, and the construction of more reliable prognostic prediction models and the further elucidation of key molecular mechanisms of tumor progression are topics in urgent need of in-depth investigation. Expression patterns and clinical significance of TRAF2 were determined through bioinformatics analysis, real-time qPCR, Western Blot, immunohistochemistry. GSEA analysis, transmission electron microscopy, 2D/3D colony formation assay, cell migration and invasion assay, and tube-formation assay were used to investigate the underlying function and mechanism of the TRAF2/M2 macrophage/autophagy axis. As one of the constituent genes of the risk model, TRAF2 was determined to be upregulated in ccRCC and associated with poor clinical prognosis. TRAF2 promotes malignant progression of ccRCC by regulating macrophage polarization, migration and angiogenesis. Mechanistically, TRAF2 promotes the polarization of M2 macrophages, and this chemotaxis is achieved in an autophagy-dependent pathway.

Western Blot analysis of TRAF2.jpg

Fig1. Western Blot analysis of TRAF2 expression in 16 paired ccRCC and adjacent noncancerous tissues.

the association between TRAF2 and the activation of autophagy pathway.jpg

Fig2. GSEA analysis was performed in TCGA-KIRC cohort to reveal the association between TRAF2 and the activation of autophagy pathway.

Case Study 2: Xue Liang, 2023

Dysregulated expression of TRAF2 has been reported in a variety of human cancers. Whether and how TRAF2 regulates the growth of liver cancer cells remains elusive. The goal of this study is to investigate potential dysregulation of TRAF2 and its biological function in liver cancer. Here TRAF2 is up-regulated in human liver cancer cell lines and tissues, and high TRAF2 expression is associated with a poor prognosis of HCC patients. Proteomics profiling along with Co-immunoprecipitation analysis revealed that p62 is a new substrate of TRAF2, which is subjected to TRAF2-induced polyubiquitination via the K63 linkage at the K420 residue. TRAF2 depletion inhibited growth and survival of liver cancer cells both in vitro and in vivo by causing p62 accumulation, which is partially rescued by simultaneous p62 knockdown. Mechanistically, TRAF2-mediated p62 polyubiquitylation activates the mTORC1 by forming the p62-mTORC1-Rag complex, which facilitates the lysosome localization of mTORC1. TRAF2 depletion inhibited mTORC1 activity through the disruption of interaction between p62 and the mTORC1 complex.

The correlation between TRAF2 expression and overall survival in HCC patients.jpg

Fig3. The correlation between TRAF2 expression and overall survival in HCC patients.

TRAF2 promotes K63 polyubiquitination of p62 at K420site.jpg

Fig4. TRAF2 promotes K63 polyubiquitination of p62 at K420site.

TRAF2 involved in several pathways and played different roles in them. We selected most pathways TRAF2 participated on our site, such as MAPK signaling pathway, NF-kappa B signaling pathway, Sphingolipid signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with TRAF2 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
MAPK signaling pathwayTAOK2;FGF12A;DDIT3;STMN1;CASP3A;PRKACBA;HSPA1A;RAC2;FGF17
NF-kappa B signaling pathwayCcl21c;TNFSF14;BIRC2;CD40LG;LAT;GM10591;CFLAR;TRADD;PTGS2
Sphingolipid signaling pathwayLASS1;SPHK2;TRAF2;PPP2R5E;PLCB2;MAPK10;PPP2R1B;S1PR2;ASAH2
Protein processing in endoplasmic reticulumMOGS;RBX1;UBE2G1B;DNAJC1;PDIA4;CAPN2L;UBE2E2;CAPN2B;CALR
ApoptosisHIST1H1D;ATM;CDKN2A;BADB;DSG1;PRKACBB;XIAP;PLEC;HIST1H1E
Osteoclast differentiationMAPK13;MITF;NOX3;IFNAR1;BLNK;IKBKG;FOSL2;FCGR2A;PPP3CC
RIG-I-like receptor signaling pathwayMAPK8A;MAPK14A;IFNA7;ATG5;MAPK13;IKBKG;IFNA3;IFNA21;RNF207B
TNF signaling pathwayCREB3L1;Ccl12;MAP2K1;MAP3K14;CXCL5;MAPK10;ICAM1;BCL3;AKT2
Adipocytokine signaling pathwayPOMCA;TNFRSF1A;NFKB1;JAK2A;IKBKG;PRKAB1;RXRGB;RXRG;LEPA
Non-alcoholic fatty liver disease (NAFLD)NDUFB7;COX7A2;COX7B2;UQCRC2;Fasl;NDUFB9;MAPK9;NDUFA5;NDUFB8
Hepatitis CTBK1;SOCS3;EIF3E;MAPK13;DDX58;OAS2;TRADD;CXCL8;TNFRSF1A
Herpes simplex infectionFasl;CRFB2;CUL1;SRSF6A;IFNA12;TLR2;IL1B;POU2F3;ARNTL
Epstein-Barr virus infectionPOLR3D;PSMD13;CD38;IKBKG;SFPI1;YWHAG;POLR2C;HLA-E;RAN
Pathways in cancerTGFBR2;RAC3;LAMC2;PTCH1;ERBB2;LPAR6;ARHGEF12;ITGA2;FGF12
Viral carcinogenesisHIST1H4H;HDAC11;CREB3L1;UBR4;IRF7;GTF2H3;HIST1H2BN;SRF;PIK3R3
Small cell lung cancerRB1;ITGA2;BCL2L1;CYCS;AKT2;NOS2;LAMA3;XIAP;BCL2

TRAF2 has several biochemical functions, for example, CD40 receptor binding, enzyme binding, identical protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by TRAF2 itself. We selected most functions TRAF2 had, and list some proteins which have the same functions with TRAF2. You can find most of the proteins on our site.

Function Related Protein
CD40 receptor bindingTRAF2;CD40LG
enzyme bindingNOXO1;LGALS9;BLZF1;SUPT5H;EHHADH;DIS3L;HINFP;TH;BACE1
identical protein bindingMYOM1;VPS4B;LCP1;CLDND;ALK;EPS15;PPPDE2;LYZ;SRPX2
ligase activityMYLIPB;SIAH2L;RNF152;TRAF6;SH3RF1;RNF19A;TRIM27;MYLIPA;SMURF1
mitogen-activated protein kinase kinase kinase bindingMAP4K2;MAPK8IP3;PPEF2;DUSP19;MAP2K1;MARVELD3;TCF3;MAPK1;STK38
protein bindingGPRASP2;FBXO32;MOBKL2B;FXYD7;MBNL3;GNL3L;APC2;PRKDC;S100A10
protein complex bindingRNF40;BCAP31;PTK2B;RIPK1;ABP1;SLC27A5;UQCRC1;DLG4;MYO7A
protein kinase bindingZC3HC1;SHC3;PRKRIP1;BCAR1;CALM1;IL12RB2;SLC12A5;MAG;H2AFY
protein phosphatase bindingANAPC5;RPA2;SKAP1;Itga10&Itgb1;JUP;GNB2L1;KCNN4;CDH2;PPP6R3
signal transducer activityGNA15.1;NPFFR1L2;VMN1R46;OLFCG4;GNAI1;GPR177;STMN1;TAAR8A;MC5RB
sphingolipid bindingTRAF2;S1PR1
thioesterase bindingCALM2;TRAF2;TRAF4;TRAF3;ADRA2A;ARF6;RAC1;TRAF5;HAUS7
tumor necrosis factor receptor bindingTNFSF4;TNFSF11;TNF;TNFSF8;TNFSF15;TRAF4;TNFSF13B;TRIM37;CD70
ubiquitin protein ligase bindingBECN1;DIO2;PCBP2;SMC6;PIAS1;UBE2L3;HERC2;PRDX6;GABARAPL1
ubiquitin-protein transferase activityUBE2A;RNF4;MKRN1;RNF182;UBE3C;RNF213;MARCH8;TTC3;HECTD1
zinc ion bindingISL2A;ZFHX3;TRIM109;RARGB;MT2A;PJA2;TRIM35-7;PJA1;PPARG

TRAF2 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with TRAF2 here. Most of them are supplied by our site. Hope this information will be useful for your research of TRAF2.

BIRC2; BIRC3

Research Area

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11/11/2022

    Efficient peptide synthesis, accelerates our peptide-focused projects.

    10/07/2021

      Reliable antibody validation, vital for our experimental success.

      02/21/2021

        Accurate mass spectrometry analysis, critical for our proteomics studies.

        Q&As (7)

        Ask a question
        What are the mechanisms by which TRAF2 modulates stress response pathways in cells? 11/18/2022

        TRAF2 modulates cellular stress response pathways, helping cells adapt to environmental and internal stressors.

        What role does TRAF2 play in regulating apoptosis and cell survival signals? 10/03/2022

        TRAF2 plays a crucial role in cell survival and apoptosis, balancing signals to determine cell fate.

        How does TRAF2 mediate signal transduction in the TNF receptor pathway, particularly in inflammatory responses? 06/21/2020

        TRAF2 is a key mediator in the TNF receptor signaling pathway, regulating inflammatory responses and cytokine production.

        How does TRAF2 influence the development and progression of cancer, especially in terms of tumor cell survival and proliferation? 05/06/2019

        TRAF2 affects cancer progression, influencing tumor cell survival, proliferation, and resistance to apoptosis.

        How does TRAF2 contribute to immune system regulation, particularly in adaptive immune responses? 11/20/2018

        TRAF2 is involved in immune system regulation, affecting both innate and adaptive immune responses.

        How do alterations in TRAF2 expression or function affect the body's response to infections and pathogens? 09/19/2018

        Changes in TRAF2 function impact the body's ability to respond to infections, affecting immune defense mechanisms.

        What is the impact of TRAF2 dysregulation on the pathogenesis of autoimmune diseases? 07/11/2018

        Dysregulation of TRAF2 can contribute to the development of autoimmune diseases by altering immune responses.

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