As a well-defined and economical eukaryotic expression system for both secretory and intracellular expression, yeast protein expression system is perfectly suitable for large-scale manufacture of recombinant proteins. Creative BioMart has developed yeast expression vectors that are optimized for the efficient production of heterologous proteins. We have also a few strong signal sequences to ensure the highest secretion level.
All of below advantages make yeast expression system an ideal host for producing large quantities of properly folded proteins in a short period of time:
High yield
High productivity
Genetics can be easily manipulated
Chemically defined media
Complex post-translational modifications
Stable production strains
Durability
Ease of scaling up
Low protein production cost
At Creative BioMart, our expertise in protein expression extends to both Saccharomyces. cerevisiae and Pichia. pastoris expression systems. Expression of recombinant proteins in S. cerevisiae can be done using three types of vectors:
YIp
Yeast integrating plasmid
Replicating single copy plasmid
For integration into genomic locus
YCp
Yeast centromeric plasmid
Replicating single copy plasmid
For complementation studies
YEp
Yeast episomal plasmid
Replicating multi-copy plasmid
For recombinant protein production, multicopy suppression studies, etc.
Saccharomyces. cerevisiae:
Full genomic sequence known
Well developed genetic methods
About 6000 genes
A model to other eukaryotes
GRAS
No toxins
Ability to secrete proteins
Capacity for post-translational modifications
Besides, methylotrophic Pichia.pastoris is a distinguished system for its growth to very high cell densities, for the available strong and tightly regulated promoters, and for the options to produce gram amounts of recombinant protein per liter of culture. If you're looking for protein production via fed-batch pichia processes, we have the experienced and credentialed staff to move your project forward.
Creative BioMart’s custom production service offers choices of constitutive and inducible expressions with process optimization and scale up development, suitable for research projects at any stage or industry manufacture scale.
Step
Service Description
Timeline
Deliverables
1
Gene synthesis plus codon optimization
1-2 weeks
Purified protein [supplied in liquid or lyophilized form]
Feel free to send us description of your target or desired outcome of the project, our scientist will guide you through the project from design to delivery and provide expert consulting at no additional charge.
Contact us or send an email at for project quotations and more detailed information.
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