Active Recombinant Human MAP3K11(1-468) Protein, N-GST-tagged

• Cat.No.: MAP3K11-154H
• Product Overview: N-terminal GSTtagged, recombinant, human MLK3 amino acids 1-468 expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Specification

• Description: MLK3 is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 1-468 aa
• Form: 0.76mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 62U/mg, where one unit of MLK3 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 79 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSKEFM EPLKSLFLKS 241 PLGSWNGSGS GGGGGGGGGR PEGSPKAAGY ANPVWTALFD YEPSGQDELA LRKGDRVEVL 301 SRDAAISGDE GWWAGQVGGQ VGIFPSNYVS RGGGPPPCEV ASFQELRLEE VIGIGGFGKV 361 YRGSWRGELV AVKAARQDPD EDISVTAESV RQEARLFAML AHPNIIALKA VCLEEPNLCL 421 VMEYAAGGPL SRALAGRRVP PHVLVNWAVQ IARGMHYLHC EALVPVIHRD LKSNNILLLQ 481 PIESDDMEHK TLKITDFGLA REWHKTTQMS AAGTYAWMAP EVIKASTFSK GSDVWSFGVL 541 LWELLTGEVP YRGIDCLAVA YGVAVNKLTL PIPSTCPEPF AQLMADCWAQ DPHRRPDFAS 601 ILQQLEALEA QVLREMPRDS FHSMQEGWKR EIQGLFDELR AKEKELLSRE EELTRAAREQ 661 RSQAEQLRRR EHLLAQWELE VFERELTLLL QQVDRER
• Purity: Purity 90% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP3K11 mitogen-activated protein kinase kinase kinase 11 [ Homo sapiens (human) ]
• Official Symbol: MAP3K11
• Synonyms: MAP3K11; mitogen-activated protein kinase kinase kinase 11; MLK3; PTK1; SPRK; MLK-3; MEKK11
• Gene ID: 4296
• mRNA Refseq: NM_002419.4
• Protein Refseq: NP_002410.1
• MIM: 600050
• UniProt ID: Q16584