MAP3K5

  • Official Full Name

    mitogen-activated protein kinase kinase kinase 5

  • Overview

    Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
  • Synonyms

    MAP3K5; mitogen-activated protein kinase kinase kinase 5; ASK1; MEKK5; MAPKKK5; apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; OTTHUMP00000017275; MAP/ERK kinase kinase 5;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Human
  • Mouse
  • Zebrafish
  • E.coli
  • HEK293
  • HEK293T
  • Insect Cell
  • Mammalian Cell
  • Mammalian cells
  • Sf9 Insect Cell
  • Flag
  • GST
  • GST,His
  • His
  • His (Fc)
  • Avi
  • His|T7
  • Myc
  • DDK
  • Myc|DDK
  • N/A
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MAP3K5-578H Active Recombinant Human MAP3K5, GST-tagged Sf9 Insect Cell GST
Human MAP3K5-25H Active Recombinant Human MAP3K5(649-946) Protein, N-GST,C-His-tagged E.coli GST,His 649-946 aa
Human MAP3K5-63H Recombinant Human MAP3K5 protein, Flag-tagged, Biotinylated Insect Cell Flag
Human MAP3K5-1260H Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase 5, GST-tagged Insect Cell GST
Human MAP3K5-11H Recombinant Human MAP3K5 protein, His-tagged E.coli His Gly681~Glu936
Human MAP3K5-245H Recombinant Human MAP3K5, GST-tagged, Active Sf9 Insect Cell GST
Human MAP3K5-20H Recombinant Human MAP3K5 protein, MYC/DDK-tagged HEK293 Myc/DDK
Human MAP3K5-26518TH Recombinant Human MAP3K5 N/A
Human MAP3K5-4504HCL Recombinant Human MAP3K5 293 Cell Lysate HEK293 N/A
Human MAP3K5-361HFL Recombinant Full Length Human MAP3K5 Protein, C-Flag-tagged Mammalian cells Flag
Human MAP3K5-1093H Recombinant Human MAP3K5 Protein (M649-K946), GST/His tagged E.coli GST/His M649-K946
Human MAP3K5-1092H Recombinant Human MAP3K5 Protein (M649-K946), His tagged E.coli His M649-K946
Human MAP3K5-1359H Recombinant Human MAP3K5 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human MAP3K5-1359H-B Recombinant Human MAP3K5 Protein Pre-coupled Magnetic Beads HEK293
Mouse MAP3K5-9515M Recombinant Mouse MAP3K5 Protein Mammalian Cell His
Mouse ASK1-3585M Recombinant Mouse ASK1, His-tagged, T7 tagged E.coli His/T7 1380
Mouse MAP3K5-5333M Recombinant Mouse MAP3K5 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Map3k5-3931M Recombinant Mouse Map3k5 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse MAP3K5-5333M-B Recombinant Mouse MAP3K5 Protein Pre-coupled Magnetic Beads HEK293
Zebrafish MAP3K5-7528Z Recombinant Zebrafish MAP3K5 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is MAP3K5 protein?

Mitogen-Activated Protein Kinase Kinase Kinase 5, or MAP3K5 protein for short, this particular protein carries an array of responsibilities in several critical cellular processes. The discovery of MAP3K5 dates back to studies undertaken in the early 1990s where it was found to be among the family of serine/threonine-protein kinases and is encoded by the MAP3K5 gene. Conserved across several species, MAP3K5 is scientifically recognized as MKK Kinase, sometimes referred to as Apoptosis Signal-regulating Kinase 1 (ASK1) due to its essential role in cellular death processes.

The MAP3K5 gene in humans is found on the chromosome 6, precisely at the locus 6q22.33 which highlights the physical and genetic position of where the protein does originate from an individual's genetic makeup.

Protein Structure

The MAP3K5 protein structure comprises several functional domains. It includes a C-terminal region with an important kinase domain that contains a catalytic core structure typical of serine/threonine-protein kinases. Notably, it also includes a serine-rich region associated with regulatory phosphorylation sites, N-terminal domain holds a protein-protein interaction domain coiled-coil, and a nuclear export signal (NES) and nuclear localization signal (NLS) sequences.

What are the functions of MAP3K5 protein?

Functionally, MAP3K5 protein engages in various processes driving essential functions of life in living organisms. This protein acts as a nodal point in the MAPK signaling pathway, receiving signals from various receptors and delivering them to downstream effectors. It activates two main subfamilies of MAP2Ks: MKK4/MKK7 (which activate JNK pathway) and MKK3/MKK6 (which activate the p38 pathways).

MAP3K5 protein plays a crucial role in numerous physiological processes ranging from apoptosis, inflammation, response to oxidative stress, cell differentiation to immune response. This protein is pivotal in mediating apoptosis (programmed cell death), a vital process in human health and disease. During oxidative stress, MAP3K5 is activated, leading to apoptosis for the preservation of organismic integrity.

MAP3K5 Protein Related Signal Pathway

The MAP3K5 protein plays a critical role in the MAPK signaling pathway. The Mitogen-activated protein kinase (MAPK) pathway is a biochemical route in the cell that transmits information from the cell surface to the DNA in the nucleus, ultimately influencing genetic activity. The MAPK pathways are vital for various cellular functions including survival, proliferation, differentiation, and apoptosis.

MAP3K5 protein, as a component of the MAPK signaling pathway, functions at the upstream level where it receives and processes signals before transmitting them to downstream components. The protein is involved in the activation of both the p38 MAPK and the JNK MAP kinase pathways.

MAP3K5 Protein Related Diseases

utation or deviation from normal functionality of MAP3K5 protein has links with several diseases. Given its central role in a variety of physiological processes, abnormalities in the MAP3K5 protein can lead to complexities. Evidence suggests it in the pathophysiology of neurodegenerative diseases like Alzheimer's and Parkinson's disease. In Alzheimer's disease, for example, the role of MAP3K5 protein in neuronal death presents the possibility of it being a potential therapeutic target.

Additionally, aberrations in the MAP3K5 protein and its pathways have been implicated in cancer biology. Studies have shown that MAP3K5 protein might endorse tumor growth in certain types of cancer, such as lung and breast cancer. Notably, more studies are needed in this area to fully elucidate causal or correlational relationships.

MAP3K5 Protein's Applications

Applications of MAP3K5 protein lie predominantly within the research and medicinal fields. As it plays a pivotal role in diseases like Parkinson's, Alzheimer's, and certain cancers, it is the subject of rigorous studies aiming to establish new treatment modalities. The protein's role in critical pathways and processes has drawn scientific interest, making it a target for drug discovery and development.

Targeting the MAP3K5 protein could potentially mitigate the progression of neurodegenerative diseases and slow cancer growth. Nevertheless, while the promise is evident, further research is required to ensure these treatments' safety and efficacy, considering the MAP3K5 protein's broad impact across various cellular functions.

  • ancer research: Map3k5, also known as apoptosis signal-regulating kinase 1 (ASK1), is involved in regulating cell proliferation and survival. Dysregulation of Map3k5 signaling has been implicated in various types of cancers. Targeting Map3k5 signaling pathway may have potential therapeutic implications in cancer treatment.
  •  Neurodegenerative diseases: Map3k5 signaling has been linked to neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Understanding the role of Map3k5 in neuronal cell death and neuroinflammation may help in the development of novel therapeutic strategies for these conditions.
  •  Cardiovascular diseases: Map3k5 has been implicated in the development and progression of cardiovascular diseases, including hypertension and atherosclerosis. Modulating Map3k5 signaling pathway may provide new insights into the treatment of these conditions.

High Purity

SDS-PAGE MAP3K5-11H.jpg

Fig1. SDS-PAGE (Cat. No.: MAP3K5-11H)

Survival curves spanning the period from baseline.jpg

(Brian J. Morris, 2021)

Fig2. Survival curves spanning the period from baseline (1991–1993) to Dec 31, 2019 for men with and men without a CMD according to genotypes of MAP3K5 SNP rs2076260. (A) Survival curves for men with a CMD vs. men without a CMD for major allele homozygote (CC) vs. minor allele carriers (CT+TT) (p=0.000041 and p=0.95, respectively).

Kaplan-Meier survival analysis.jpg

(Hanning Wang, 2023)

Fig3. Kaplan-Meier survival analysis of eight OS-related genes. (I) Results of GSVA in the high- and low-risk groups.

MAP3K5 involved in several pathways and played different roles in them. We selected most pathways MAP3K5 participated on our site, such as MAPK signaling pathway, Sphingolipid signaling pathway, Protein processing in endoplasmic reticulum, which may be useful for your reference. Also, other proteins which involved in the same pathway with MAP3K5 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
MAPK signaling pathwayMAPT;PRKCG;CACNA1C;HRASB;FGF5;HRAS;BRAF;FGF6B;MAP3K7
Sphingolipid signaling pathwayFYN;PPP2R2D;DEGS2;TNFRSF1A;TRAF2;ASAH2;MAPK3;PPP2R2A;FCER1A
Protein processing in endoplasmic reticulumUBQLN3;ATXN3;CKAP4;BCAP31;CALR;EIF2AK2;DERL1;SEL1L;EIF2AK1
TNF signaling pathwayCXCL2;CCL5;MAPK13;IKBKB;MAPK3;CXCL1;NFKBIA;VCAM1;MAP3K7
Neurotrophin signaling pathwayIRS1;PIK3CD;SHC4;RAPGEF1;AKT1;NFKB1;SOS1;RPS6KA6;TP53
Non-alcoholic fatty liver disease (NAFLD)IRS1;NFKB1;NDUFA6;NDUFB10;INS;NDUFB2;MLXIPL;TRAF2;SDHD
Amyotrophic lateral sclerosis (ALS)APAF1;BID;CASP12;ALS2;BCL2L1;PPP3R2;TP53;BAD;PPP3CC

MAP3K5 has several biochemical functions, for example, ATP binding, MAP kinase kinase kinase activity, magnesium ion binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MAP3K5 itself. We selected most functions MAP3K5 had, and list some proteins which have the same functions with MAP3K5. You can find most of the proteins on our site.

Function Related Protein
ATP bindingHARS2;STK4;MLH1;GMPS;MAP3K13;MYH7;DDX17;DNAJA2;DNAJA3A
MAP kinase kinase kinase activityMAP3K6;MAP3K5;RAF1;MAP3K3;YSK4;BRAF;MAP3K8;MAP3K2;MOS
magnesium ion bindingGTPBP5;MTHFD2;IMPAD1;RRAGC;NLRC4;SIK3;RPS6KA6;EPHX2;ARF1
protein bindingSKA1;KLF3;ARPIN;RPF1;SVIP;ERP44;CXADR;GRIK5;KRTAP10-8
protein homodimerization activityKIAA1804;SEPHS1;DMRTA1;SRR;ANG;ARID3A;DMRTB1;ANO2;CD2
protein kinase activityPIK3CG;NTRK2B;PDK3;KDRL;TGFBR1A;MAP3K3;DYRK1A;ADRBK1;PHKG1B
protein kinase bindingCHEK2;SPDYE2;PXN;RHEB;CCNT2A;DUSP3;POLA1;PTPRR;GATA6
protein phosphatase bindingROS1;JAK3;JAK1;LCK;CDKN1B;RPA3;DRD1;GNB2L1;ITGA1

MAP3K5 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MAP3K5 here. Most of them are supplied by our site. Hope this information will be useful for your research of MAP3K5.

DAXX; YWHAZ; N/A; ZNF622

Research Area

Related articles

Izykowska, K; Zawada, M; et al. Submicroscopic genomic rearrangements change gene expression in T-cell large granular lymphocyte leukemia. EUROPEAN JOURNAL OF HAEMATOLOGY 93:143-149(2014).
Cole, JB; Waurich, B; et al. A genome-wide association study of calf birth weight in Holstein cattle using single nucleotide polymorphisms and phenotypes predicted from auxiliary traits. JOURNAL OF DAIRY SCIENCE 97:3156-3172(2014).
  • Q&As
  • Reviews

Q&As (5)

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What other diseases or conditions involve MAP3K5? 12/19/2022

MAP3K5 has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions.

How is MAP3K5 protein implicated in cancer? 08/05/2022

Aberrant regulation of MAP3K5 has been associated with cancer development, as it can influence cell survival and death pathways.

Can MAP3K5 be used as a biomarker for certain diseases? 09/09/2021

MAP3K5 expression levels and activity may serve as biomarkers for specific diseases, providing valuable information for diagnosis and prognosis.

Are there any clinical applications of MAP3K5 in cancer treatment? 02/02/2018

Research suggests that targeting MAP3K5 could have therapeutic implications in cancer treatment, potentially through the modulation of apoptosis in cancer cells.

Are there ongoing clinical trials involving MAP3K5 inhibitors? 10/24/2017

Investigational studies and clinical trials are underway to explore the efficacy of MAP3K5 inhibitors in different disease contexts, including cancer.

Customer Reviews (3)

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Reviews
06/30/2020

    The MAP3K5 protein has proven to be invaluable in protein electron microscopy structure analysis.

    07/24/2018

      Its ability to provide accurate and reliable results in this particular application makes it a preferred choice for researchers studying antigen-antibody interactions and immune responses.

      12/01/2016

        The MAP3K5 protein's sensitivity and specificity ensure precise measurements, contributing to the overall success of ELISA experiments.

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