• Official Full Name

    mitogen-activated protein kinase kinase 1

  • Overview

    The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
  • Synonyms

    MAP2K1; mitogen-activated protein kinase kinase 1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1; dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1);

  • Recombinant Proteins
  • Protein Pre-coupled Magnetic Beads
  • Cell & Tissue Lysates
  • Assay Kits
  • Chicken
  • Human
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • Zebrafish
  • E.coli
  • HEK293
  • HEK293T
  • Insect (sf21)
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Sf9 Insect Cell
  • DDK
  • Flag
  • Gly
  • Pro
  • GST
  • GST,His
  • His
  • His (Fc)
  • Avi
  • His|GST
  • His|MBP
  • Myc
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MAP2K1-2568H Active Recombinant Human MAP2K1, His-tagged Insect Cell His
Human MAP2K1-175H Active Recombinant Human MAP2K1, GST-tagged E.coli GST
Human MAP2K1-105HFL Unactive Recombinant Full Length Human MAP2K1 Protein, N-GST,C-His-tagged E.coli GST,His Full Length
Human MAP2K1-104HFL Active Recombinant Full Length Human MAP2K1 Protein, N-GST,C-His-tagged E.coli GST,His Full Length
Human MAP2K1-343H Recombinant Human MAP2K1 Mammalian Cell His
Human MAP2K1-639H Recombinant Human MAP2K1 protein, His-tagged E.coli His
Human MAP2K1-596H Recombinant Human MAP2K1, His-tagged Sf9 Insect Cell His
Human MAP2K1-1034H Recombinant Human Mitogen-Activated Protein Kinase Kinase 1, GST-tagged E.coli GST
Human MAP2K1-1033H Recombinant Human Mitogen-Activated Protein Kinase Kinase 1, GST-tagged Sf9 Insect Cell GST
Human MAP2K1-167H Recombinant Human v protein, MYC/DDK-tagged HEK293 Myc/DDK
Human MAP2K1-62H Recombinant Human MAP2K1 protein, Flag-tagged, Biotinylated Insect Cell Flag
Human MAP2K1-1035H Recombinant Human Mitogen-Activated Protein Kinase Kinase 1, His-tagged Sf9 Insect Cell His
Human MAP2K1-299H Recombinant Human MAP2K1 protein, His/MBP-tagged E.coli His/MBP 1-91 aa
Human MAP2K1-300H Recombinant Human MAP2K1 protein, His/MBP-tagged E.coli His/MBP 181-393 aa
Human MAP2K1-2723HF Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged Insect (sf21) GST 1-393(end)
Human MAP2K1-8116HF Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged E.coli GST 1-393(end)
Human MAP2K1-3929HF Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged Insect (sf21) GST 1-393(end)
Human MAP2K1-234HFL Recombinant Full Length Human MAP2K1 Protein, C-Flag-tagged Mammalian cells Flag
Human MAP2K1-2354HF Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged Insect (sf21) GST 1-393(end)
Human MAP2K1-3430H-B Recombinant Human MAP2K1 Protein Pre-coupled Magnetic Beads HEK293
Human MAP2K1-9955HF Active Recombinant Full Length Human MAP2K1 Protein, DDK-tagged, Biotinylated Insect (sf21) DDK 1-393(end)
Human MAP2K1-5001H Recombinant Human MAP2K1 Protein (Leu54-Val369), N-His tagged E.coli N-His Leu54-Val369
Human MAP2K1-5481HF Active Recombinant Full Length Human MAP2K1 Protein, GST-tagged Insect (sf21) GST 1-393(end)
Human MAP2K1-3430H Recombinant Human MAP2K1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Map2k1-1032M Active Recombinant Mouse Map2k1 protein, GST-tagged E.coli GST full-length
Mouse Map2k1-666M Active Recombinant Mouse Map2k1, GST-tagged E.coli GST Full Length
Mouse Map2k1-1032H Recombinant Mouse Mitogen-Activated Protein Kinase Kinase 1, GST-tagged E.coli GST
Mouse Map2k1-1350M Recombinant Mouse Map2k1, His & GST tagged Insect Cell His/GST
Mouse MAP2K1-483M Recombinant Mouse MAP2K1, Gly & Pro tagged Insect Cell His
Mouse MAP2K1-001MCL Recombinant Mouse MAP2K1 cell lysate Insect Cell N/A
Mouse MAP2K1-483MAF488 Recombinant Mouse Map2k1 Protein, Gly/Pro-tagged, Alexa Fluor 488 conjugated Insect Cells Gly/Pro 395
Mouse Map2k1-3920M Recombinant Mouse Map2k1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse MAP2K1-1310M Recombinant Mouse MAP2K1 protein(Met1-Ile393), His&GST-tagged Insect Cells N-His&GST Met1-Ile393
Mouse Map2k1-1350MAF488 Recombinant Mouse Map2k1 Protein, His/GST-tagged, Alexa Fluor 488 conjugated Insect Cells His/GST 630
Mouse Map2k1-1350MAF555 Recombinant Mouse Map2k1 Protein, His/GST-tagged, Alexa Fluor 555 conjugated Insect Cells His/GST 630
Mouse Map2k1-1350MF Recombinant Mouse Map2k1 Protein, His/GST-tagged, FITC conjugated Insect Cells His/GST 630
Mouse MAP2K1-483MAF555 Recombinant Mouse Map2k1 Protein, Gly/Pro-tagged, Alexa Fluor 555 conjugated Insect Cells Gly/Pro 395
Mouse MAP2K1-1307M Recombinant Mouse MAP2K1 protein(Met1-Ile393) Insect Cells N/A Met1-Ile393
Mouse Map2k1-1350MAF647 Recombinant Mouse Map2k1 Protein, His/GST-tagged, Alexa Fluor 647 conjugated Insect Cells His/GST 630
Mouse MAP2K1-483MAF647 Recombinant Mouse Map2k1 Protein, Gly/Pro-tagged, Alexa Fluor 647 conjugated Insect Cells Gly/Pro 395
Mouse MAP2K1-483MF Recombinant Mouse Map2k1 Protein, Gly/Pro-tagged, FITC conjugated Insect Cells Gly/Pro 395
Rat MAP2K1-3559R Recombinant Rat MAP2K1 Protein Mammalian Cell His
Rat MAP2K1-3215R-B Recombinant Rat MAP2K1 Protein Pre-coupled Magnetic Beads HEK293
Rat MAP2K1-3215R Recombinant Rat MAP2K1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque MAP2K1-2663R Recombinant Rhesus monkey MAP2K1 Protein, His-tagged Mammalian Cell His
Rhesus Macaque MAP2K1-2483R-B Recombinant Rhesus Macaque MAP2K1 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque MAP2K1-2483R Recombinant Rhesus Macaque MAP2K1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Zebrafish MAP2K1-12591Z Recombinant Zebrafish MAP2K1 Mammalian Cell His
Chicken MAP2K1-1266C Recombinant Chicken MAP2K1 Mammalian Cell His
Kit-1614 MEK1 Kinase Binding Assay Kit N/A
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Map2k1 Related Signal Pathway

What is MAP2K1 protein?

The protein encoded by MAP2K1 gene is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein (MAP) kinase kinase. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade.

Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. Depending on the cellular context, this kinase is involved in diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

MAP2K1 related signaling pathway

The RAS-MAPK pathway: The RAS-MAPK pathway is a signal transduction cascade that plays a crucial role in normal cellular processes such as cell growth, proliferation, differentiation, survival, metabolism and migration. The pathway is stimulated by growth factors that lead to RAS-mediated RAF activation. This kinase phosphorylates MEK1 and/or MEK2 with consequent ERK1/2 activation, downstream the cascade. ERK1 and ERK2 effectors act both in nucleus and in cytoplasm, mediating the polymorphic cellular response to growth factors.

The ERK-MAPK pathway: Extensive studies have demonstrated the importance of the ERK-MAPK pathway in promoting early commitment and differentiation of skeletal progenitors to the osteoblast lineage and skeletal mineralization.

The mTOR pathway: The ERK-MAPK pathway controls the mTOR pathway, a signaling modulator of mitochondrial biogenesis that regulates cellular energy metabolism. ERK inhibition by an MEK inhibitor activates the mTORC2-AKT signaling axis downstream of epidermal growth factor.

The Hippo pathway: The findings show that MEK1 promotes YAP protein expression through beta-transducin repeat containing E3 ubiquitin protein ligase (BTRC). Dysfunctional Hippo/YAP signaling pathway has been linked to hepatocarcinogenesis. Further research found that YAP and MEK1 were closely correlated in tumor samples.

MAP2K1 Related Diseases

Cardiofaciocutaneous syndrome 3 (CFC3) : It is an autosomal dominant syndrome, caused by heterozygous pathogenic variants in the genes BRAF, MAP2K1/MEK1, MAP2K2/MEK2, KRAS or, rarely, YWHAZ, all part of the RAS-MAPK pathway. A form of cardiofaciocutaneous syndrome, a multiple congenital anomaly disorder characterized by a distinctive facial appearance, heart defects and intellectual disability.

Melorheostosis, isolated (MEL): MAP2K1 mutations inhibit BMP2-mediated osteoblast mineralization and differentiation in vitro, underlying the markedly increased osteoid detected in affected bone histology. A sclerosing bone disorder characterized by hyperostosis of the cortex of tubular bones, frequently involving one limb.

Other malignant disease: MAP2K1 is invlved in diverse pathways, once any related pathway anomalies, it can cause diseases of varying degrees like histiocytosis in Langerhans cell, tumorigenesis in liver cancer and melanoma so on.


Fig1. Mutations of MAP2K1 in histiocytoses. Description of all mutation detected, corresponding to either (A) SBSs or (B) DELINs. Correlation of the type of genetic alteration with age and subtype of histiocytosis. C1: SBSs and C2: DELINs.

High Purity



Fig2. The activity was determined by ELISA. (MAP2K1-2723HF)

Case Study 1: Lanlan Li, 2013


Fig1. Up-regulation of MEK1 in liver cancer tissues. Western blotting of proteins as indicated in a mix pool of liver cancer and corresponding adjacent normal tissues from 15 patients.

Case Study 2: Thanaset Senawong, 2008


Fig2. The following vectors were independently transfected: pcDNA3.1 (empty), WT, K97M (catalytically inactive), ΔN3 (active), S218D+S222D (constitutively active), S218A+S222A (constitutively inactive) MEK1, F53S and Y130C MEK1 (found in CFC).


G Protein coupled Receptors Signaling


MAPK Signaling Pathway

Map2k1 involved in several pathways and played different roles in them. We selected most pathways Map2k1 participated on our site, such as MAPK signaling pathway, ErbB signaling pathway, Ras signaling pathway, which may be useful for your reference. Also, other proteins which involved in the same pathway with Map2k1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
MAPK signaling pathwayPPM1A;MAP3K13;ARR3B;MRAS;HSP70L;MAPK12;MAP4K3;GNA12A;LRRK2
Ras signaling pathwayFGF14;KSR2;PIK3CA;SHC2;PAK3;KITLG;PLD1;FGF18;RASAL1
cAMP signaling pathwayCREB3L3;VAV1;PIK3R2;PIK3CA;ATP1A1;ATP2B4;MAP2K1;MAPK1;GPR81
Chemokine signaling pathwayVAV1;PIK3CG;PLCB2;PIK3CD;GRK6;WAS;GRK4;CCL4;FGR
HIF- signaling pathwayPIK3R2;RPS6;HK2;NOX3;PIK3CG;CAMK2D;RBX1;FLT1;EPO
Sphingolipid signaling pathwayPPP2R5E;MAPK3;PPP2R1A;BID;S1PR2;NOS3;SPHK2;PIK3R2;S1PR5
Phospholipase D signaling pathwayAGPAT4;INS2;RHOA;PDGFRB;RAF1;MAP2K1;AVP;INS;PLCB4
Vascular smooth muscle contractionNPR2;CALM1B;ADCY1B;PPP1R12C;CALML3;PTGIR;ADORA2AA;Adcy4;PRKCBA
Dorso-ventral axis formationGRB2;NOTCH3;SPIRE2;CPEB1A;SPIRE1;PIWIL1;CPEB2;CPEB3;NOTCH4
Osteoclast differentiationLILRA3;CYBB;MAPK10;NFKB2;IFNGR1;PPP3R1;FOS;FCGR3;FYN
Signaling pathways regulating pluripotency of stem cellsWNT8A;ACVR1C;WNT5B;ID3;WNT8B;PCGF5;PCGF1;STAT3;HESX1
Toll-like receptor signaling pathwayAKT2L;RAC1;STAT1B;TLR9;TBK1;IFNA10;PIK3R3A;CASP8;TRAF3
Natural killer cell mediated cytotoxicityKLRC1;PLCG1;IFNA8;MAP2K2;KIR2DL3;NFATC2;PIK3R3;TNFRSF10B;HLA-C
T cell receptor signaling pathwayNFATC1;PPP3R1;PAK7;MUC1;CD3G;VAV1;IL4;PIK3CA;RAF1
B cell receptor signaling pathwayCd79a, Cd79b;LIME1;AKT2;MAP4K1;INPP5D;CRK;PIK3R3;VAV2;HRAS
Fc epsilon RI signaling pathwayRAC1;PLA2G4F;FYN;MS4A2;IL5;IL13;IL3;SYK;FCER1G
Fc gamma R-mediated phagocytosiscgr2b;PLA2G6;ARPC5L;PPAP2B;ASAP1;AKT2;PIP5K1B;SPHK2;NCF1
TNF signaling pathwayMMP9;CCL2;CASP7;BAG4;MAP3K5;PIK3R3;Casp3;SELE;CCL5
Neurotrophin signaling pathwayPIK3CB;PTPN11;ZNF274;NFKB1;NRAS;CALM1;SH2B1;RPS6KA1;PRKCD
Cholinergic synapseBCL2;CHRM1;PIK3CB;AKT2;KCNJ2;ITPR1;KCNJ6;SLC5A7;Adcy4
Long-term depressionLYN;IGF1;PRKG2;PLA2G4C;GNA12;MAPK3;GRID2;GRIA1;ITPR1
Regulation of actin cytoskeletonITGAX;ITGB2;MYL2B;PAK6;CDC42;ARPC5B;PPP1CBL;FGF18;EGFRA
Insulin signaling pathwayMAPK10;SOCS1;PPP1R3B;PPP1CBL;FBP1;PRKCZ;RAF1;PHKB;SHC3
Progesterone-mediated oocyte maturationMAD1L1;MAPK8B;GNAI1;GNAI2A;MAP2K1;MAPK8;MAPK9;PIK3R3A;PIK3CB
Estrogen signaling pathwayHSPA1L;HRAS;MAP2K2;GNAS;FKBP4;GABBR1;SHC2;NOS3;PLCB4
Prolactin signaling pathwayMAPK1;SOCS5;PIK3CA;NRAS;SHC4;STAT5B;FOS;TH;MAP2K2
Thyroid hormone signaling pathwayNCOR1;MAPK3;WNT4;PIK3CG;MED27;RCAN1;PLCB1;MED24;CASP9
Prion diseasesC8A;C1QC;CASP12;STIP1;MAPK1;C6;PRNP;CCL5;BAX
Proteoglycans in cancerPLAU;WNT10B;MMP2;HSPB2;PLCE1;MRAS;RHOA;CAV3;THBS1
Renal cell carcinomaMAP2K1;EGLN1;ETS1;CDC42;PAK6;TGFB1;PAK2;GAB1;PIK3R1
Non-small cell lung cancerPRKCG;PIK3R3;KRAS;FHIT;BAD;ERBB2;PIK3CG;PDPK1;MAP2K2
Central carbon metabolism in cancerFGFR1;PDHA2;FGFR2;MAP2K1;PFKM;HRAS;PDHB;SIRT6;PGAM1
Choline metabolism in cancerPIP5K1B;TSC1;WASF2;GPCPD1;PLD2;PDGFRB;WASF1;TSC2;MAP2K1

Map2k1 has several biochemical functions, for example, ATP binding, MAP kinase kinase activity, Ras GTPase binding. Some of the functions are cooperated with other proteins, some of the functions could acted by Map2k1 itself. We selected most functions Map2k1 had, and list some proteins which have the same functions with Map2k1. You can find most of the proteins on our site.

Function Related Protein
MAP kinase kinase activityMAP3K9;MAP2K2A;PAK3;MAPKAPK5;MAP2K1;MAP2K2B;ALPK2;MAP2K6;MAP2K3
mitogen-activated protein kinase kinase kinase bindingMAP3K11;DUSP19;MAPK9;TRAF2;TRAF6;PPEF2;MAPK8IP3;TCF3;MAP4K2
protein C-terminus bindingCTGF;CDK7;HSPG2;VIM;TRP53;ZBTB16;DYNLL1;KPNA3;DST
protein N-terminus bindingPEX19;SMARCC1;TDRD7;NPAT;CHMP6;ERCC3;STX5A;DCN;DCTN4
protein serine/threonine kinase activator activityMAP2K2;ALS2;SPRY2;IGF2;STRADA;CALM3;STK4;LTF;STK3
protein serine/threonine kinase activityACVRL1;TAOK1;PHKG2;PLK2B;RPS6KC1;MINK1;SGK1;ATM;MELK
protein serine/threonine/tyrosine kinase activityAURKC;CLK4;PRKAA2;TESK2;DYRK1A;AURKB;AURKA;CLK2;DYRK3
protein tyrosine kinase activityEPHA4B;IGF1RB;MAP2K7;PTK7;SHC1;MAP2K3;KDRL;TNK2B;CLK2
receptor signaling protein tyrosine phosphatase activity

Map2k1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Map2k1 here. Most of them are supplied by our site. Hope this information will be useful for your research of Map2k1.


  • Reviews
  • Q&As

Customer Reviews (3)

Write a review

    The unmatched performance of the MAP2K1 protein in both ELISA assays and protein electron microscopy structure analysis showcases its versatility and reliability in various experimental settings.


      The MAP2K1 protein finds extensive utility in protein electron microscopy structure analysis.


        With its exceptional specificity and sensitivity, it enables precise and reliable detection of target molecules, making it a valuable tool in the field of diagnostic and research applications.

        Q&As (5)

        Ask a question
        Are there ongoing clinical trials targeting MAP2K1? 01/12/2023

        Yes, there are clinical trials exploring the efficacy and safety of MAP2K1 inhibitors in various cancers and other diseases.

        Are there diagnostic tests specifically targeting MAP2K1 in clinical practice? 01/10/2023

        Some diagnostic tests may analyze MAP2K1 mutations, especially in the context of cancer, to inform treatment decisions.

        What diagnostic applications does MAP2K1 have in cancer detection? 09/30/2019

        MAP2K1 mutations can serve as biomarkers in cancer diagnostics, aiding in the identification and classification of tumors.

        How does MAP2K1 contribute to drug resistance in cancer treatment? 06/14/2019

        MAP2K1 mutations can be associated with resistance to certain cancer therapies, making it important to understand its role in treatment response.

        Are there targeted therapies based on MAP2K1 in cancer treatment? 02/17/2016

        Yes, MAP2K1 inhibitors are being explored as targeted therapies in cancer treatment, aiming to block the abnormal signaling associated with MAP2K1 mutations.

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