MAP2K6

  • Official Full Name

    mitogen-activated protein kinase kinase 6

  • Overview

    MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase. MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK.
  • Synonyms

    MAP2K6; mitogen-activated protein kinase kinase 6; PRKMK6; dual specificity mitogen-activated protein kinase kinase 6; MAPKK6; MEK6; MKK6; protein kinase; mitogen activated; kinase 6 (MAP kinase kinase 6); SAPKK3; MEK 6; MAPKK 6; SAPK kinase 3; MAPK/ERK k; SAPKK-3;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Human
  • Mouse
  • Rhesus Macaque
  • Zebrafish
  • Baculovirus-Insect Cells
  • E.coli
  • HEK293
  • HEK293T
  • Insect (sf21)
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Sf9 Insect Cell
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • SUMO
  • MBP
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human MAP2K6-176H Active Recombinant Human MAP2K6, GST-tagged E.coli GST
Human MAP2K6-147H Active Recombinant Human MAP2K6(S599D, T603D)(4-end) Mutation Protein, N-MBP-tagged E.coli MBP 4-end
Human MAP2K6-889H Active Recombinant Human MAP2K6 protein (Met 1-Asp 334 (Ser 207/Asp, Thr 211/Asp)), His & GST-tagged Baculovirus-Insect Cells His & GST
Human MAP2K6-149H Unactive Recombinant Human MAP2K6(4-end) Protein, N-MBP-tagged E.coli MBP 4-end
Human MAP2K6-148H Active Recombinant Human MAP2K6(4-end) Protein, N-MBP-tagged E.coli MBP 4-end
Human MAP2K6-525H Recombinant Human MKK6, Gly & Pro tagged Insect Cell His
Human MAP2K6-97H Recombinant Human MAP2K6, His-tagged Insect Cell His
Human MAP2K6-888H Active Recombinant Human MAP2K6 protein, His&GST-tagged Insect Cells His/GST Met1-Asp334,Ser207/Asp,211Thr/Asp
Human MAP2K6-34H Recombinant human biotinylated MAP2K6, His-tagged E.coli His
Human MAP2K6-524H Recombinant Human MKK6, Gly & Pro tagged Insect Cell His
Human MAP2K6-30189TH Recombinant Human MAP2K6 Insect Cell N/A
Human MAP2K6-47H Recombinant Human MEK6, GST-tagged Sf9 Insect Cell GST
Human MAP2K6-001HCL Recombinant Human MAP2K6 cell lysate Insect Cell N/A
Human MAP2K6-6391H Recombinant Human MAP2K6 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human MAP2K6-9864HF Active Recombinant Full Length Human MAP2K6 Protein, GST-tagged E.coli GST 1-334(end)
Human MAP2K6-3059H Recombinant Human MAP2K6 protein, His-tagged E.coli His 1-334 aa
Human MAP2K6-01H Recombinant Human MAP2K6 Protein, His-tagged Insect Cell His
Human MAP2K6-2241H Recombinant Human MAP2K6 protein, His-tagged Insect Cell His 1-334aa
Human MAP2K6-440HFL Active Recombinant Full Length Human MAP2K6 Protein, C-Flag-tagged Mammalian cells Flag
Human MAP2K6-4007HF Active Recombinant Full Length Human MAP2K6 Protein, GST-tagged E.coli GST 1-334(end)
Human MAP2K6-1355H-B Recombinant Human MAP2K6 Protein Pre-coupled Magnetic Beads HEK293
Human MAP2K6-3203H Recombinant Human MAP2K6 protein, His-SUMO-tagged E.coli His-SUMO 1-334aa
Human MAP2K6-2189H Recombinant Human MAP2K6 protein Insect Cells N/A Met1-Asp334,207Asp,211Asp
Human MAP2K6-1355H Recombinant Human MAP2K6 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human MAP2K6-5007H Recombinant Human MAP2K6 Protein (Phe46-Pro306), N-His tagged E.coli N-His Phe46-Pro306
Human MAP2K6-2159HF Active Recombinant Full Length Human MAP2K6 Protein, GST-tagged Insect (sf21) GST 1-334(end)
Human MAP2K6-392H Recombinant Human MAP2K6 Protein, MYC/DDK-tagged HEK293 Myc/DDK
Mouse MAP2K6-9503M Recombinant Mouse MAP2K6 Protein Mammalian Cell His
Mouse MAP2K6-5326M-B Recombinant Mouse MAP2K6 Protein Pre-coupled Magnetic Beads HEK293
Mouse Map2k6-3924M Recombinant Mouse Map2k6 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse MAP2K6-5326M Recombinant Mouse MAP2K6 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque MAP2K6-2665R Recombinant Rhesus monkey MAP2K6 Protein, His-tagged Mammalian Cell His
Rhesus Macaque MAP2K6-2485R Recombinant Rhesus Macaque MAP2K6 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rhesus Macaque MAP2K6-2485R-B Recombinant Rhesus Macaque MAP2K6 Protein Pre-coupled Magnetic Beads HEK293
Zebrafish MAP2K6-9245Z Recombinant Zebrafish MAP2K6 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
MAP2K6-7.jpg

Fig1. Proposed model for involvement of MKK6–p38 MAPK pathway in the regulation of melanocyte dendrites.

What is MAP2K6 protein?

MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6) is a protein coding gene which situated on the long arm of chromosome 17 at locus 17q24. This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. The MAP2K6 protein is consisted of 334 amino acids and its molecular mass is approximately 37.5 kDa. The secreted recombinant human MKK6 consists of 336 amino acids and predicts a molecular mass of 37.6 KDa. The apparent molecular mass of the protein is approximately 37 KDa in SDS-PAGE under reducing conditions due to glycosylation.

What is the function of MAP2K6 protein?

MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MAP2K6 (Mitogen activated protein kinase 6) is a mitogen-activated protein kinase kinase, which belongs to the MAPK signaling pathway. Its main function is to regulate biological processes such as cell growth, differentiation and apoptosis. Specifically, MAP2K6 can phosphorylate its downstream MAPK kinases, such as MAPK3 and MAPK6, thereby activating the activity of these kinases. These kinases further phosphorylate other proteins, including transcription factors, cytoskeletal proteins, and other signaling molecules, thereby regulating the physiological function of cells. In addition, MAP2K6 can also influence cell survival and death by regulating cell cycle and apoptosis.

MAP2K6 related Signaling pathways

MAP2K6 is involved in the MAPK signaling pathway, which includes a three-stage signaling process: MAPK, MAPK kinase (MEK or MKK), and MAPK kinase kinase (MEKK or MKKK). These three kinases can be activated sequentially, and together regulate a variety of important physiological and pathological effects of cells.

MAP2K6 can also open heterochromatin by phosphorylation of Gatad2b, improve the histone acetylation modification in the promoter region of pluripotent genes, and finally improve the expression of pluripotent genes and promote the acquisition of pluripotent genes.

MAP2K6 Related Diseases

Mutations in MAP2K6 gene are associated with the occurrence and development of a variety of tumors, such as breast cancer, colorectal cancer, gastric cancer and so on. In addition, abnormal expression or mutation of MAP2K6 gene can lead to neurodegenerative diseases, such as Alzheimer's disease and Parkinson's disease. It is also associated with some other diseases, such as cardiovascular diseases, diabetes, cirrhosis and so on.

Bioapplications of MAP2K6

In some tumor cells, MAP2K6 expression is associated with tumor aggressiveness and poor prognosis. Therefore, MAP2K6 is considered a potential therapeutic target that could be used to develop new anticancer drugs. In addition, MAP2K6 opens heterochromatin by phosphorylation of Gatad2b, improves histone acetylation modification in the promoter region of the pluripotent gene, and ultimately improves the expression of the pluripotent gene and promotes the acquisition of pluripotency, which is also an ideal model for studying epigenetic regulation.

High Purity

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MAP2K6-2.jpg

Fig2. The activity was determined by ELISA. (MAP2K6-9864HF)

Case Study 1: Guangsuo Xing, 2022

MAP2K6-3.jpg

Fig1. Co-immunoprecipitation of Mkk6 and Gatad2b indicated interaction between them.

Case Study 2: Aristóbolo M. Silva, 2004

MAP2K6-4.jpg

Fig2. Following 48 h of mock-transfection (NT) or transfection with pCEFL-GST-tagged MKK6 construct, 293T cells were treated with pIC (100 μg/ml) for the times as indicated.

MAP2K6 involved in several pathways and played different roles in them. We selected most pathways MAP2K6 participated on our site, such as MAPK signaling pathway, Rap signaling pathway, Osteoclast differentiation, which may be useful for your reference. Also, other proteins which involved in the same pathway with MAP2K6 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
MAPK signaling pathwayCACNA1BA;IL1B;CACNG6A;FLNB;STK4;RAC3B;HRASA;MAP4K1;PAK2
Rap signaling pathwayACTG1;PLCE1;PRKD3;FGF16;PDGFA;RAP1B;LPAR5;GRIN2A;PRKD1
Osteoclast differentiationPPP3R2;cgr2b;CYBB;FCGR3B;PIK3R1;LILRB2;FCGR3A;SYK;NCF2
Toll-like receptor signaling pathwayIFNPHI1;NFKBIA;CXCL8B.3;NFKBIAB;MAP2K2;IL12B;RAC1;RIPK1;MAPK10
Fc epsilon RI signaling pathwayGRB2;FCER1G;SYK;MAP2K6;NRAS;PLA2G4D;RAC2;RAF1;MAP2K2
TNF signaling pathwayPIK3CB;RPS6KA4;ATF4;MAP3K5;PIK3R5;VEGFC;CCL20;CREB3L2;TAB2
Inflammatory mediator regulation of TRP channelsPPP1CA;CAMK2B;PRKCH;ITPR1;MAP2K6;CAMK2A;PRKACG;CALM1;ASIC3
GnRH signaling pathwayLHB;PTK2B;PLA2G4A;ITPR1;MAP2K1;CALM4;GNA11;HRASA;ITPR3
Amyotrophic lateral sclerosis (ALS)CASP1;BID;DERL1;BCL2L1;GRIN2A;PPP3CB;TNF;PPP3CC;PPP3R2
ToxoplasmosisHLA-DPA1;MAPK8;MAPK13;PIK3CD;HLA-DRB4;IRGM;H2-AA;PPIF;TLR2
Influenza ABLB2;IFNA10;MAP2K7;MAVS;IFNA17;NLRP3;NXT2;IFNB;IFNB1
Epstein-Barr virus infectionMAPK11;YWHAZ;IL10RA;CCNA2;CD38;YWHAH;AKT2;POLR1C;POLR2I

MAP2K6 has several biochemical functions, for example, ATP binding, MAP kinase kinase activity, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MAP2K6 itself. We selected most functions MAP2K6 had, and list some proteins which have the same functions with MAP2K6. You can find most of the proteins on our site.

Function Related Protein
ATP bindingUBE2D4;STK39;HUNK;CLK1;MCM6;SRPK1B;SETX;MCM8;BBS10
MAP kinase kinase activityLRRK2;MAP2K2B;MAPK10;MAP2K1;ALPK2;MAPK14;MAP3K9;MAP2K6;MAP2K3
protein bindingVAV2;MKKS;PRKCA;TEX14;KIRREL2;TXNDC16;SMAD3;TRIP10;CPNE3
protein kinase bindingGRIN2A;SLC12A7;USF1;DAXX;YWHAZ;RELA;DVL1;KCNA5;ADCY6
protein serine/threonine kinase activityPAK4;TSSK5;MAP4K6;STK25;MAP2K6;NEK8;RPS6KA1;BMPR2A;CSNK1A1L
protein tyrosine kinase activityKIAA1804;TESK1;ERBB2;MET;ABL1;TYRO3;MAP2K1;DSTYK;FGFR1A

MAP2K6 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MAP2K6 here. Most of them are supplied by our site. Hope this information will be useful for your research of MAP2K6.

GADD45A; MAP3K4; LRRK2; MAP2K3; RELA; PKN1; MAPK14; MAPK14; TAOK2; GADD45B; GADD45G; MLH1

  • Q&As
  • Reviews

Q&As (5)

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Is MAP2K6 a potential biomarker for predicting treatment response? 05/02/2022

Its expression levels in certain cancers are being explored as predictive biomarkers to anticipate response to specific therapies.

Is MAP2K6 involved in any other diseases besides cancer? 04/15/2021

Studies also suggest its involvement in cardiovascular diseases and neurological disorders.

How does MAP2K6 relate to drug resistance in cancer treatment? 12/06/2020

MAP2K6 signaling pathways might play a role in drug resistance mechanisms, making it a focus for overcoming therapeutic challenges.

Are there any known diseases associated with MAP2K6 mutations? 11/01/2019

Yes, mutations in MAP2K6 have been linked to certain cancers, including colorectal cancer and lung adenocarcinoma.

Can MAP2K6 inhibition lead to potential side effects? 06/26/2018

As with any targeted therapy, MAP2K6 inhibitors might have side effects, which are under investigation in preclinical and clinical studies.

Customer Reviews (3)

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Reviews
07/21/2022

    I am certain that the MAP2K6 protein will play a pivotal role in facilitating robust and insightful research, providing the assurance of reliable and consistent results.

    03/23/2022

      The excellent technical support provided by the manufacturer can solve my problem.

      12/27/2021

        Whether I am studying protein-protein interactions, investigating cellular pathways, or examining protein folding dynamics, the MAP2K6 protein consistently delivers exceptional performance and precise outcomes.

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