Species : |
Human |
Source : |
HEK293 |
Tag : |
Avi&Fc |
Protein Length : |
AA Cys 22 - Arg 221 |
Description : |
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family. |
Predicted N Terminal : |
Cys 22 |
Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7.5, 10% trehalose. |
Bio-activity : |
Immobilized Human CD48, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human 2B4, Fc Tag with a linear range of 0.16-1.25 μg/mL. |
Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 50.7 kDa. As a result of glycosylation, the protein migrates as 65-80 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE). |
Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
Purity : |
>95% as determined by SDS-PAGE. |
Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |