Active Recombinant Human CD28 Protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: CD28-3908H
• Product Overview: Biotinylated Recombinant Human CD28 Protein (Asn 19 - Pro 152) (AAH93698), fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc&Avi
• Predicted N Terminal: Asn 19
• Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human B7-2, Fc Tag at 5μg/mL (100 μL/well) can bind Biotinylated Human CD28, Fc Tag with a linear range of 0.15-5 μg/mL.
• Molecular Mass: Recombinant Human CD28 Protein has a calculated MW of 43.6 kDa. As a result of glycosylation, The protein migrates as 55-66 kDa under reducing (R) condition and 110-130 kDa under non-reducing (NR) condition on SDS-PAGE gel.
• Protein length: Asn 19 - Pro 152
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747
• Chromosome Location: 2q33
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem
• Function: SH3/SH2 adaptor activity; coreceptor activity; identical protein binding; protease binding; protein binding; protein homodimerization activity

Reviews

08/24/2021

Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

08/24/2021

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

08/06/2021

the CD28 protein's outstanding performance in ELISA and its utility in protein electron microscopy structure analysis make it a highly recommended tool for researchers.

Q&As

Can CD28 protein be used for personalized medicine?

CD28 protein expression and function may be relevant for personalized treatment strategies in autoimmune diseases and cancer.

What are the potential clinical applications of targeting CD28 protein?

Targeting CD28 protein may have therapeutic potential in treating autoimmune diseases, cancer immunotherapy, and organ transplantation.

How does CD28 protein impact regulatory T cells (Tregs)?

CD28 signaling is important for the function and maintenance of regulatory T cells, which play a role in immune tolerance and suppression of autoimmunity.

How does CD28 protein impact cancer immunotherapy?

CD28 protein is involved in enhancing the anti-tumor immune response, making it a potential target for cancer immunotherapy.

Are there any approved therapies targeting CD28 protein?

Currently, there are no FDA-approved therapies specifically targeting CD28 protein, but research is ongoing in this area.