Recombinant Mouse Cyp7b1 Protein, Myc/DDK-tagged

• Cat.No.: Cyp7b1-2426M
• Product Overview: Purified recombinant protein of mouse full-length cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response. 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 58.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cyp7b1 cytochrome P450, family 7, subfamily b, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cyp7b1
• Synonyms: CYP7B1; cytochrome P450, family 7, subfamily b, polypeptide 1; 25-hydroxycholesterol 7-alpha-hydroxylase; cytochrome P450 7B1; cytochrome P450, 7b1; oxysterol 7-alpha-hydroxylase; hippocampal transcript 1 protein; hct-1; AW261589; D3Ertd552e
• Gene ID: 13123
• mRNA Refseq: NM_007825
• Protein Refseq: NP_031851
• UniProt ID: Q60991