Recombinant Human CYP7B1 Protein, GST-tagged

• Cat.No.: CYP7B1-2292H
• Product Overview: Human CYP7B1 full-length ORF ( NP_004811.1, 1 a.a. - 506 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 84.7 kDa
• AA Sequence: MAGEVSAATGRFSLERLGLPGLALA AALLLLALCLLVRRTRRPGEPPLIK GWLPYLGVVLNLRKDPLRFMKTLQK QHGDTFTVLLGGKYITFILDPFQYQ LVIKNHKQLSFRVFSNKLLEKAFSI SQLQKNHDMNDELHLCYQFLQGKSL DILLESMMQNLKQVFEPQLLKTTSW DTAELYPFCSSIIFEITFTTIYGKV IVCDNNKFISELRDDFLKFDDKFAY LVSNIPIELLGNVKSIREKIIKCFS SEKLAKMQGWSEVFQSRQDVLEKYY VHEDLEIGAHHLGFLWASVANTIPT MFWAMYYLLRHPEAMAAVRDEIDRL LQSTGQKKGSGFPIHLTREQLDSLI CLESSIFEALRLSSYSTTIRFVEED LTLSSETGDYCVRKGDLVAIFPPVL HGDPEIFEAPEEFRYDRFIEDGKKK TTFFKRGKKLKCYLMPFGTGTSKCP GRFFALMEIKQLLVILLTYFDLEII DDKPIGLNYSRLLFGIQYPDSDVLF RYKVKS
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CYP7B1 cytochrome P450, family 7, subfamily B, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP7B1
• Synonyms: CYP7B1; cytochrome P450, family 7, subfamily B, polypeptide 1; cytochrome P450, subfamily VIIB (oxysterol 7 alpha hydroxylase), polypeptide 1 , spastic paraplegia 5A (autosomal recessive) , SPG5A; 25-hydroxycholesterol 7-alpha-hydroxylase; cytochrome P450 7B1; oxysterol 7alpha-hydroxylase; oxysterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1; CP7B; CBAS3; SPG5A
• Gene ID: 9420
• mRNA Refseq: NM_004820
• Protein Refseq: NP_004811
• MIM: 603711
• UniProt ID: O75881