What is SRPK1 Protein

SRPK1, with its official full name Serine-Arginine Protein Kinase 1, is a member of the serine/arginine-rich protein kinase family. This family plays a crucial role in the phosphorylation of serine-arginine (SR) rich proteins, a process intricately linked to pre-mRNA splicing. Synonyms for SRPK1 include SRPK, SFRS protein kinase 1, and Clk1, adding to the complexity of its nomenclature.

SRPK1 Protein Structural Characteristics and Classification

SRPK1 exhibits distinctive structural characteristics, featuring a catalytic domain responsible for its kinase activity. The protein is classified as a serine/threonine protein kinase, a subgroup within the broader protein kinase family. Recent advances in structural biology have provided insights into the three-dimensional arrangement of SRPK1, enhancing our understanding of its functional significance.

Recent Research Advances about SRPK1 Protein

Cutting-edge research has uncovered novel facets of SRPK1's role in cellular processes. Studies have elucidated its interaction with specific SR proteins, shedding light on the intricate mechanisms governing pre-mRNA splicing. Additionally, recent advances have highlighted the implications of SRPK1 dysregulation in various diseases, making it a potential therapeutic target.

SRPK1 Biological Functions and Molecular Mechanisms

SRPK1's biological functions are deeply intertwined with its role in pre-mRNA splicing. It phosphorylates SR proteins, a crucial step in regulating alternative splicing of pre-mRNAs. This process, fundamental to gene expression, contributes to the diversity of proteins generated from a single gene, impacting cellular functions and organismal development.

The molecular mechanisms orchestrated by SRPK1 involve its precise phosphorylation of serine-arginine-rich motifs within SR proteins. This phosphorylation event influences the interaction of SR proteins with the pre-mRNA and spliceosome, ultimately modulating the splicing process. Dysregulation of SRPK1 activity has been implicated in aberrant splicing events associated with various diseases.

Figure 1. SRPK1 promotes EGFR-TKI resistance. (Huang J Q, et al., 2023)

SRPK1 Related Signaling Pathway

The SRPK1-related signal pathway is intricately connected to the intricate network of cellular signaling governing pre-mRNA splicing. SRPK1's phosphorylation of SR proteins serves as a key regulatory step in this pathway, influencing downstream events in gene expression. Unraveling the details of this pathway provides crucial insights into the broader landscape of cellular regulation.

SRPK1 Related Diseases

The dysregulation of SRPK1 has been linked to several diseases, including certain cancers and neurodegenerative disorders. Abnormalities in pre-mRNA splicing, mediated by SRPK1, can lead to the production of aberrant protein isoforms, contributing to the pathogenesis of these diseases. Understanding these connections opens avenues for targeted therapeutic interventions.

SRPK1's Applications in Biomedicine

The unique role of SRPK1 in pre-mRNA splicing positions it as a potential target for biomedical applications. Researchers are exploring its applications in diagnostic development, where aberrant splicing patterns could serve as biomarkers for certain diseases. Additionally, the modulation of SRPK1 activity holds promise in vaccine development and therapeutics, providing new avenues for precision medicine.

Recommended Products

Cat.#	Product name	Species	Source (Host)	Tag
SRPK1-80H	Recombinant Human SRPK1 protein, Flag-tagged, Biotinylated	Human	Insect Cell	Flag
SRPK1-713H	Recombinant Human SFRS Protein Kinase 1, GST-His	Human	Sf9 Insect Cell	GST
SRPK1-435H	Recombinant Human SFRS Protein Kinase 1, GST-tagged, Active	Human	Sf9 Insect Cell	GST
SRPK1-1066H	Recombinant Human SRPK1 Protein (M1-S655), GST tagged	Human	E.coli	GST
SRPK1-5928HF	Active Recombinant Full Length Human SRPK1 Protein, BTN-tagged	Human	Insect (sf21)	BTN
SRPK1-30850TH	Recombinant Human SRPK1	Human		N/A
SRPK1-903H	Recombinant Human SRPK1 protein, His-GST-tagged	Human	Insect Cell	His/GST
SRPK1-6923HF	Active Recombinant Full Length Human SRPK1 Protein, GST-tagged	Human	E.coli	GST
SRPK1-1067H	Recombinant Human SRPK1 Protein (M1-S655), Tag Free	Human	E.coli	No tag
SRPK1-15995M	Recombinant Mouse SRPK1 Protein	Mouse	Mammalian Cell	His

Reference

• Huang J Q, et al. Serine-arginine protein kinase 1 (SRPK1) promotes EGFR-TKI resistance by enhancing GSK3β Ser9 autophosphorylation independent of its kinase activity in non-small-cell lung cancer. Oncogene. 2023, 42(15): 1233-1246.