CD200R1

  • Official Full Name

    CD200 receptor 1

  • Overview

    This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may contr
  • Synonyms

    CD200R1; CD200 receptor 1; OX2R; MOX2R; CD200R; HCRTR2; Orexin receptor 2; OTTMUSP00000015932; OTTMUSP00000030764; antigen identified by monoclonal antibody MRC OX-2 receptor;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Bos taurus (Bovine)
  • Chicken
  • Cynomolgus
  • Cynomolgus Monkey
  • Human
  • Mouse
  • Rat
  • CHO cells
  • E.coli
  • E.coli expression system
  • HEK293
  • HEK293T
  • Human Cell
  • Human Cells
  • In Vitro Cell Free System
  • Insect Cell
  • Mammalian Cell
  • Mammalian cells
  • Wheat Germ
  • Avi|Fc
  • C
  • His
  • hFc
  • Avi
  • DYKDDDDK
  • Fc
  • Fc Chimera
  • GST
  • HIgG
  • His (Fc)
  • Myc
  • DDK
  • Myc|DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human CD200R1-417H Active Recombinant Human CD200R1, Fc Chimera Mammalian cells Fc Chimera
Human CD200R1-051H Active Recombinant Human CD200R1 protein, Fc-Avi-tagged, Biotinylated CHO cells Fc/Avi Ala27-Leu266
Human CD200R1-3254H Active Recombinant Human CD200R1 protein, His&hFc-tagged HEK293 His&hFc Met1-Leu266
Human CD200R1-3202H Recombinant Human CD200R1 protein(Met1-Leu266), His-tagged HEK293 C-His Met1-Leu266
Human CD200R1-27295TH Recombinant Human CD200R1, GST-tagged Wheat Germ GST
Human CD200R1-347H Recombinant Human CD200R1 Protein, Fc-tagged HEK293 Fc
Human CD200R1-2523HCL Recombinant Human CD200R1 cell lysate Human Cell N/A
Human CD200R1-0988H Recombinant Human CD200R1 Protein (Met1-Leu266), C-His tagged Mammalian cells C-His Met1-Leu266
Human CD200R1-3287H Recombinant Human CD200R1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human CD200R1-3057H Recombinant Human CD200R1 Protein, MYC/DDK-tagged HEK293 Myc/DDK
Human CD200R1-348H Active Recombinant Human CD200R1 Protein, Fc-Avi-tagged, Biotinylated HEK293 Avi/Fc
Human CD200R1-01H Active Recombinant Human CD200R1 Protein, hIgG-His-Tagged Insect Cell HIgG/His 462aa
Human CD200R1-321H Recombinant Human CD200R1 Protein, His-tagged Human Cells His
Human CD200R1-172H Recombinant Human CD200R1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human CD200R1-1010H Recombinant Human CD200R1 protein, His-tagged HEK293 His Ala 27 - Leu 266
Human CD200R1-495H Active Recombinant Human CD200R1 protein, hFc-tagged HEK293 hFc Met29-Leu-243
Human CD200R1-172H-B Recombinant Human CD200R1 Protein Pre-coupled Magnetic Beads HEK293
Human CD200R1-322H Recombinant Human CD200R1 Protein, DYKDDDDK-tagged Human Cells DYKDDDDK
Human CD200R1-55HF Recombinant Full Length Human CD200R1 Protein, GST-tagged In Vitro Cell Free System GST
Human CD200R1-277H Recombinant Human CD200R1 Protein, Ala27-Leu266, C-His-Avi tagged, Biotinylated HEK293 C-His-Avi Ala27-Leu266
Mouse Cd200r1-458M Active Recombinant Mouse Cd200r1, Fc Chimera Mammalian cells Fc Chimera
Mouse Cd200r1-3280M Active Recombinant Mouse Cd200r1 protein, His-tagged HEK293 His Met1-Pro238
Mouse Cd200r1-631M Recombinant Mouse Cd200r1 protein(Met1-Pro238), His&hFc-tagged HEK293 C-His&hFc Met1-Pro238
Mouse CD200R1-2226MCL Recombinant Mouse CD200R1 cell lysate Human Cell N/A
Mouse Cd200r1-2054M Recombinant Mouse Cd200r1 Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse Cd200r1-661M Recombinant Mouse Cd200r1 Protein, His-tagged E.coli N-His Thr26-Pro238
Mouse Cd200r1-125M-B Recombinant Mouse Cd200r1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Cd200r1-125M Recombinant Mouse Cd200r1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat CD200R1-1240R Recombinant Rat CD200R1 Protein Mammalian Cell His
Rat CD200R1-38R Recombinant Rat CD200R1 Protein, His-tagged HEK293 His
Rat CD200R1-898R Recombinant Rat CD200R1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat CD200R1-898R-B Recombinant Rat CD200R1 Protein Pre-coupled Magnetic Beads HEK293
Bos taurus (Bovine) RFL9031BF Recombinant Full Length Bovine Cell Surface Glycoprotein Cd200 Receptor 1(Cd200R1) Protein, His-Tagged E.coli expression system His Full Length of Mature Protein (27-355)
Cynomolgus CD200R1-3483C Active Recombinant Cynomolgus CD200R1 Protein, His-tagged HEK293 His
Cynomolgus Monkey CD200R1-2483CCL Recombinant Cynomolgus CD200R1 cell lysate Human Cell N/A
Chicken CD200R1-3523C Recombinant Chicken CD200R1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is CD200R1 protein?

CD200R1 (CD200 receptor 1) gene is a protein coding gene which situated on the long arm of chromosome 3 at locus 3q13. This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. The CD200R1 protein is consisted of 348 amino acids and its molecular mass is approximately 39 kDa.

What is the function of CD200R1 protein?

CD200R1 is an inhibitory receptor for the CD200/OX2 cell surface glycoprotein which limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also it binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.

CD200R1 Related Signaling Pathway

CD200 is an immunosuppressive molecule that regulates the function of immune cells by binding to CD200R1. CD200R1 can activate PI3K/AKT signaling pathway and MAPK/ERK signaling pathway, thereby affecting cell survival, proliferation, metabolism and other processes. CD200R1 can regulate the stability and nuclear localization of β-catenin, the core component of Wnt/β-catenin signaling pathway, and affect the tumorigenesis and development. CD200R1 regulates the phosphorylation and stability of YAP/TAZ, a core component of the Hippo/YAP signaling pathway.

CD200R1-9.jpg

Fig1. CD200-CD200R interaction in tumor microenvironment. (Jin-Qing Liu, 2020)

CD200R1 Related Diseases

CD200R1 protein plays an important role in immune regulation, and the abnormal function of CD200R1 may lead to the overactivation of immune cells and the dysregulation of immune response, resulting in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus. Abnormal expression of CD200R1 protein may affect the body's immune response to pathogens, leading to uncontrolled inflammatory response and even increasing the risk of infection. It is also associated with the occurrence and development of some tumors and neurological diseases.

CD200R1-10.jpg

Fig2. CD200R and CD200 were positively correlated in hepatocellular carcinoma (HCC) patients.

Bioapplications of CD200R1

The CD200R1 protein is also used in diagnosis. For example, in the diagnosis of breast cancer, the expression level of CD200R1 protein can be used as an important indicator to judge the malignancy and prognosis of the tumor. This diagnostic method has been widely used in clinical practice. The development of drugs to target it is also under way.

High Purity

.

CD200R1-2.jpg

Fig2. Activity Data. (CD200R1-051H)

Case study 1:Xiao-Guang Luo, 2010

Microglia are the representative myeloid cells in the brain, and their over-activation plays an important role in the pathogenesis of Parkinson's disease (PD). Microglia activation is believed to be regulated by the CD200-CD200R signaling. As the peripheral counterpart of microglia, monocyte-derived macrophages (MDMs) share the same progenitor and antigen markers, and they have similar biological behaviors and mirror microglial function in the brain.

Here, the team studied CD200R expression and its regulation in MDMs from 32 PD cases, 27 age-matched old controls, and 28 young controls. We found that the basal CD200R expression is similar in MDMs from young control, old control and PD patients. The study indicates that inducible CD200R expression correlated inversely with the onset age of PD and to tumor necrosis factor-alpha (TNF-alpha) released from MDMs. These results suggest an intrinsic abnormality in the CD200-CD200R signaling in MDMs during aging and, especially, in PD.

CD200R1-3.jpg

Fig1. Correlation between CD200R response and the onset ages of PD patients.

CD200R1-4.jpg
Fig2. Correlation between CD200R and TNF-a release in MDMs.

Case study 2: Seung-Phil Shin, 2021

CD200 is known as an immune checkpoint molecule that inhibits innate immune cell activation. Using a head and neck squamous cell carcinoma (HNSCC) model, this team sought to determine whether localized delivery of adenovirus-expressing sCD200R1-Ig, the soluble extracellular domain of CD200R1, enhances antitumor immunity.

CD200 is known as an immune checkpoint molecule that inhibits innate immune cell activation. Using a head and neck squamous cell carcinoma (HNSCC) model, we sought to determine whether localized delivery of adenovirus-expressing sCD200R1-Ig, the soluble extracellular domain of CD200R1, enhances antitumor immunity. Adenovirus-expressing sCD200R1-Ig (Ad5sCD200R1) was designed, and its effect was tested. CD200 induced M2-like polarization both in vitro and in vivo. These protumor effects of CD200 were driven through the β-catenin/NF-κB/M-CSF axis. Ad5sCD200R1 injection could be an effective targeted strategy to enhance antitumor immunoediting.

CD200R1-5.jpg

Fig3. MEER/CD200High cells were infected at MOIs of 10 and 100 for 2 h to confirm the secretion of soluble sCD200R1-Ig (sCD200R1-Ig) into the culture medium.

CD200R1-6.jpg
Fig4. The localization of phosphorylated p65 was monitored by confocal microscopy.
CD200R1-8.jpg

Fig2. CD200-CD200R axis is considered to be a pair of checkpoint molecules that regulate tumor-specific immune responses. (Jin-Qing Liu, 2020)

CD200R1 involved in several pathways and played different roles in them. We selected most pathways CD200R1 participated on our site, such as Adaptive Immune System, Immune System, Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, which may be useful for your reference. Also, other proteins which involved in the same pathway with CD200R1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Adaptive Immune SystemBLMH;NCR3LG1;LRR1;CMTM2A;FGF18B;AP2A2;CTSK;LRSAM1;AGO2
Immune SystemGM11711;POLR3H;CLEC2B;IFIT2;PRLRB;KIFAP3;KIF4A;SEC24A;SIGIRR
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cellLILRA2;TREM1;OSCAR;CRTAM;CD300C;SIGLEC12;KLRG1;NCR3LG1;CD200R2

CD200R1 has several biochemical functions, for example, protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by CD200R1 itself. We selected most functions CD200R1 had, and list some proteins which have the same functions with CD200R1. You can find most of the proteins on our site.

Function Related Protein
protein bindingTJAP1;PLCE1;ZC3H15;TAF13;DLL4;NECAB2;ITGB1BP1;S100A10;CERK

CD200R1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CD200R1 here. Most of them are supplied by our site. Hope this information will be useful for your research of CD200R1.

CD200

Research Area

Related articles

Nicholls, SM; Copland, DA; et al. Local targeting of the CD200-CD200R axis does not promote corneal graft survival. EXPERIMENTAL EYE RESEARCH 130:1-8(2015).
Gorczynski, R; Yu, K; et al. Anti-CD200R2, Anti-IL-9, Anti-IL-35, or Anti-TGF-A Abolishes Increased Graft Survival and Treg Induction Induced in Cromolyn- Treated CD200R1KO. CD200tg Mice. TRANSPLANTATION 97:39-46(2014).
  • Q&As
  • Reviews

Q&As (7)

Ask a question
 Is CD200R1 a potential therapeutic target for immune-related disorders? 02/22/2023

Modulating CD200R1 signaling holds therapeutic potential for immune-related disorders. Enhancing CD200R1 signaling or agonizing CD200R1 with specific agonists could help dampen excessive immune responses, attenuate inflammation, and promote immune tolerance. Conversely, inhibiting CD200R1 signaling or blocking CD200-CD200R1 interaction may be beneficial in conditions where immune suppression is desired, such as cancer immunotherapy. However, further research is needed to develop safe and effective therapeutic strategies targeting CD200R1, taking into consideration the potential impact on immune cell functions and overall immune homeostasis.

Are there any disease associations with CD200R1 dysregulation? 07/28/2021

Dysregulation of CD200R1 signaling has been implicated in the pathogenesis of several diseases. Reduced CD200R1 expression or impaired CD200-CD200R1 interaction has been observed in autoimmune disorders, such as multiple sclerosis and rheumatoid arthritis. This dysregulation can lead to excessive activation of immune cells and chronic inflammation. Furthermore, altered CD200R1 expression has been reported in neuroinflammatory conditions, such as Alzheimer's disease and Parkinson's disease. However, more studies are required to elucidate the specific roles of CD200R1 dysregulation in disease development and progression.

What is the cellular localization of CD200R1 protein in the body? 01/01/2021

 CD200R1 (CD200 receptor 1) is primarily expressed on the surface of various immune cells, including macrophages, dendritic cells, and mast cells. It is also found on some non-immune cells, such as neurons. The specific cellular localization of CD200R1 allows it to interact with its ligand, CD200, which is expressed on many cell types, including neurons, endothelial cells, and immune cells. This interaction plays a crucial role in regulating immune responses and maintaining immune homeostasis.

What is the role of CD200R1 in immune regulation and tolerance? 03/10/2020

CD200R1 plays a critical role in immune regulation and tolerance. The interaction between CD200R1 on immune cells and its ligand CD200 on target cells provides a potent inhibitory signal, suppressing immune cell activation and effector functions. This regulation helps prevent autoimmune responses and excessive inflammation. CD200R1 signaling inhibits the activation of immune cells, such as macrophages and dendritic cells, thereby reducing their pro-inflammatory cytokine production and antigen presentation capacity. Dysregulation of CD200R1 signaling has been associated with autoimmune diseases and neuroinflammatory disorders.

How is CD200R1 expression regulated in immune cells? 03/14/2019

 CD200R1 expression is regulated by various factors, including cytokines and transcriptional regulators. Pro-inflammatory cytokines, such as interferon-gamma, can downregulate CD200R1 expression on immune cells, whereas anti-inflammatory cytokines, such as interleukin-4, can upregulate its expression. Transcription factors, such as PU.1 and GATA-1, are involved in regulating CD200R1 gene expression. Additionally, epigenetic modifications, such as DNA methylation and histone acetylation, can influence CD200R1 expression levels. Further research is needed to fully understand the complex regulatory mechanisms governing CD200R1 expression.

What are the signaling pathways activated upon CD200R1-CD200 interaction? 06/22/2018

CD200R1 engagement with its ligand CD200 triggers inhibitory signaling pathways, primarily mediated by immunoreceptor tyrosine-based inhibitory motifs (ITIMs) present in the CD200R1 cytoplasmic domain. Upon ligand binding, CD200R1 recruits and activates phosphatases, such as SHP-1 and SHP-2, which dephosphorylate downstream signaling molecules. This leads to the inhibition of various immune cell functions, including phagocytosis, cytokine production, and antigen presentation. The CD200R1-CD200 signaling axis is crucial for maintaining immune tolerance and preventing excessive immune activation.

What are the downstream signaling pathways activated by CD200R1? 07/15/2016

Upon binding of CD200 to CD200R1, several downstream signaling pathways are activated. One of the main pathways involves the recruitment and activation of Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1 and SHP-2. These phosphatases inhibit signaling cascades initiated by immune receptors, including inhibitory signaling through the inhibitory receptor CD200R1 itself. Additionally, CD200R1 engagement can also lead to the modulation of PI3K-Akt and MAPK pathways, further influencing immune cell function and cytokine production.

Customer Reviews (3)

Write a review
Reviews
05/25/2020

    The convenience it offers saves a considerable amount of time and effort.

    04/10/2020

      The robustness of this reagent guarantees consistent results, regardless of the number of experiment replications.

      06/07/2016

        Purity personified, unlocking the gates to triumphant experiments.

        Ask a Question for All CD200R1 Products

        Required fields are marked with *

        My Review for All CD200R1 Products

        Required fields are marked with *

        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends