PDE6B

  • Official Full Name

    phosphodiesterase 6B, cGMP-specific, rod, beta

  • Overview

    Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE),;resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell.;PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the;beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night;blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
  • Synonyms

    PDE6B; phosphodiesterase 6B, cGMP-specific, rod, beta; PDEB; rod cGMP-specific 3,5-cyclic phosphodiesterase subunit beta; congenital stationary night blindness 3; autosomal dominant; CSNB3; rd1; RP40; GMP-PDE beta; rod cGMP-phosphodiesterase beta-subunit;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Mouse
  • E.coli
  • HEK293
  • Mammalian Cell
  • Sf9 Insect Cell
  • GST
  • His
  • His (Fc)
  • Avi
  • N/A
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human PDE6B-1530H Active Recombinant Human PDE6B, GST-tagged Sf9 Insect Cell GST Full length
Human PDE6B-3346HCL Recombinant Human PDE6B 293 Cell Lysate HEK293 N/A
Human PDE6B-3295H Recombinant Human PDE6B protein, His-tagged E.coli His 198 - 428 aa
Human PDE6B-150H Recombinant Human PDE6B protein, GST-tagged E.coli GST 1-88 aa
Mouse PDE6B-12559M Recombinant Mouse PDE6B Protein Mammalian Cell His
Mouse PDE6B-6588M Recombinant Mouse PDE6B Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse PDE6B-6588M-B Recombinant Mouse PDE6B Protein Pre-coupled Magnetic Beads HEK293
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein

What is pde6b protein?

The PDE6B protein, part of the phosphodiesterase family of proteins, plays a crucial role in the phototransduction pathway in cells, contributing significantly to vision science on a cellular level. This protein is primarily created by the PDE6B gene, providing indispensable insight into multiple facets of cellular reproduction and function.

The PDE6B protein was discovered while professionals were looking into genetic causes of certain types of blindness. The connection between PDE6B and retinal degeneration sparked interest in understanding more about the protein and its function, opening new avenues for research. With increased attention to the protein and the gene that encodes it, scientists have successfully managed to gain a comprehensive background of the protein's structure and mechanism of action.

The PDE6B gene, which encodes the PDE6B protein, is located on the human chromosome 4, locus 4p16.3. The protein structure of PDE6B, as predicted from its genetic composition, appears to be relatively complex. It features multiple domains including a phosphorylation domain, a GAF domain, and a catalytic domain. The GAF domain stands out as it has a cyclic GMP (cGMP) binding site, indicating the protein's ability to bind cGMP, an essential messenger molecule in the phototransduction pathway.

What are the functions of PDE6B protein?

The principal function of the PDE6B protein is to act as a catalyst in the breakdown of cGMP in the eye's photoreceptor cells. Photoreceptor cells, particularly rods, contain a significant concentration of the PDE6B protein, critical in the vision process. The PDE6B protein acts by catalyzing the hydrolysis of cGMP to 5' GMP, ultimately lowering the intracellular concentration of cGMP and causing the closure of cGMP-gated ion channels. This results in hyperpolarization and subsequent signal generation that allows the vision process to proceed.

PDE6B Protein Related Signal Pathway

From a signaling perspective, PDE6B plays a vital role in the phototransduction pathway - the process by which light is converted into electrical signals within the rod cells of the retina. Upon absorbing light, the photoreceptor pigment, rhodopsin, gets activated. Activated rhodopsin, in turn, activates the enzyme transducin. It then stimulates PDE6B, which hydrolyzes intracellular cGMP, closing cGMP-gated channels. This change in membrane potential generates an electrical signal transmitted through the optic nerve to the brain, facilitating the perception of vision.

PDE6B Protein Related Diseases

Mutations in the PDE6B gene and, subsequently, the protein can lead to several ocular diseases, most notably retinitis pigmentosa (RP). RP is a type of inherited retinal degeneration that leads to progressive vision loss due to the rod photoreceptor cells' destruction. Additionally, PDE6B-related mutations have also been implicated in congenital stationary night blindness and certain types of Leber's congenital amaurosis.

PDE6B Protein's Applications

Understanding the function and structure of the PDE6B protein has several significant applications. Firstly, it has helped shape our understanding of the phototransduction cascade, leading to advancements in vision science. Identification of mutations in the PDE6B gene linked to ocular diseases has enabled genetic testing for at-risk individuals and families, leading to early intervention and potential treatments.

In terms of therapeutic interventions, the PDE6B protein has been a target of interest. Gene therapy aiming to restore normal PDE6B function in affected individuals is one area of active research. For instance, viral vectors are engineered to deliver a normal copy of the PDE6B gene to photoreceptor cells, allowing the production of functional protein and potentially slowing or halting disease progression.

High Purity

SDS-PAGE PDE6B-150H.jpg

Fig1. SDS-PAGE (Cat. No.: PDE6B-150H)

the positions of mutations in rd1 and Pde6b-KO mice.jpg

(Angelina V. Chirinskaite, 2023)

Fig2. the positions of mutations in rd1 and Pde6b-KO mice; the sequence of sgRNA spacer is underlined, and amino acid substitution and nucleotide substitutions are shown in green.

HE-stained paraffin-embedded sections of Pde6bH620Q mutant.jpg

(Richard J. Davis, 2008)

Fig3.H&E-stained paraffin-embedded sections of Pde6bH620Q mutant (P14 –P49) and wildtype control (P16 and P50) mice. Shown are the outer segments (OS) photorecep-tors (PR) and inner nuclear layer (INL). Blue arrowheads: mutant photoreceptor layer after P35.

PDE6B involved in several pathways and played different roles in them. We selected most pathways PDE6B participated on our site, such as Purine metabolism, Phototransduction, which may be useful for your reference. Also, other proteins which involved in the same pathway with PDE6B were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Purine metabolismPOLD1;CANT1A;PRIM2;GMPR;ADKB;PGM1;PDE7A;DCK;POLR3F
PhototransductionCALM3B;GUCA1A;PDE6G;CALM;CALM1B;GUCA1D;GRK1;CALM4;CNGA1

PDE6B has several biochemical functions, for example, 3,5-cyclic-GMP phosphodiesterase activity, metal ion binding. Some of the functions are cooperated with other proteins, some of the functions could acted by PDE6B itself. We selected most functions PDE6B had, and list some proteins which have the same functions with PDE6B. You can find most of the proteins on our site.

Function Related Protein
3,5-cyclic-GMP phosphodiesterase activityPDE6H;PDE9A;PDE10A;PDE5A;PDE6A;PDE6G;PDE6B;PDE11A;PDE6C
metal ion bindingGALNT11;CDK15;NUBP2;TSHZ3;NRP2B;STEAP2;ZNF521;RIMS1;ACY3.1

PDE6B has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PDE6B here. Most of them are supplied by our site. Hope this information will be useful for your research of PDE6B.

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Are there any other ocular conditions linked to PDE6B dysfunction? 12/02/2021

Besides retinitis pigmentosa, PDE6B mutations have been associated with certain forms of cone-rod dystrophy.

How does PDE6B research contribute to our understanding of vision? 12/13/2020

Studying PDE6B helps unravel the molecular mechanisms involved in vision, providing insights into potential therapeutic targets.

Can PDE6B gene therapy restore vision in individuals with retinitis pigmentosa? 04/27/2019

Early studies suggest that gene therapy targeting PDE6B has the potential to slow down or even restore vision in some cases of retinitis pigmentosa.

Is PDE6B exclusively expressed in the retina? 07/04/2018

While PDE6B is primarily expressed in the retina, low levels of expression have been detected in other tissues.

Can PDE6B be used as a diagnostic marker for retinal disorders? 02/24/2016

PDE6B mutations can be identified through genetic testing, aiding in the diagnosis of retinal disorders.

Customer Reviews (3)

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Reviews
03/26/2021

    To further elevate my confidence, the manufacturer's commitment to offering exemplary technical support is truly commendable.

    01/15/2021

      Coupled with the manufacturer's commitment to impeccable technical support, I am equipped with the necessary tools to overcome any hurdles and achieve significant breakthroughs in my scientific pursuits.

      06/15/2017

        Its purity, stability, and functional integrity are of the highest standard, instilling confidence in generating accurate and insightful results crucial for my research objectives.

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