Unactive Recombinant Full Length Human MAP2K1 Protein, N-GST,C-His-tagged

• Cat.No.: MAP2K1-105HFL
• Product Overview: N-terminal GST, and C-terminal 6His-tagged, recombinant fulllength human MEK1 expressed in E.coli cells. Purified using glutathione-agarose followed by Ni2+/NTA-agarose.

Specification

• Description: ERKs (extracellular signal-regulated kinase) are activated by the MEK (mitogen-activated protein kinase, or ERK kinase). MEKs are activated by members of the Raf family. The signaling pathway comprising Raf and ERK (extracellular signal-regulated kinase) lies downstream of the small guanine nucleotide binding protein Ras and mediates several apparently conflicting cellular responses, such as proliferation, apoptosis, growth arrest, differentiation, and senescence, depending on the duration and strength of the external stimulus and on cell type. An inhibitor assay may be of benefit for the treatment of cancer, diabetes, neurodegenerative disorders or asthma. EMBL Z30163, possessing amino acid substitution A327G. Please note, introduction of the A327G mutation to the rabbit sequence (Z30163) results in expression of recombinant MEK1 protein that is 100% identical to wild-type human MEK1 described in SwissProt Q02750, note this SwissProt entry fails to record M1 and starts at P2.
• Source: E. coli
• Species: Human
• Tag: GST,His
• Protein length: Full Length
• Form: 2.957mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 5% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.
• Bio-activity: Less than 1% of the value obtained with MEK1 that has been activated with c-Raf. MEK1 activity determined by activation of 1μM MAP kinase 2.
• Molecular Mass: 71 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD IEGRGIPRSA MPKKKPTPIQ 241 LNPAPDGSAV NGTSSAETNL EALQKKLEEL ELDEQQRKRL EAFLTQKQKV GELKDDDFEK 301 ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY 361 GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD 421 VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG 481 LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI 541 FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEVD 601 FAGWLCSTIG LNQPSTPTHA AGVHHHHHH
• Purity: Purity 76.8% using SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP2K1 mitogen-activated protein kinase kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MAP2K1
• Synonyms: MAP2K1; mitogen-activated protein kinase kinase 1; MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Gene ID: 5604
• mRNA Refseq: NM_002755.4
• Protein Refseq: NP_002746.1
• MIM: 176872
• UniProt ID: Q02750