Active Recombinant Full Length Human MAP2K2 Protein, N-His-tagged

• Cat.No.: MAP2K2-106HFL
• Product Overview: N-terminal 6His-tagged recombinant, full-length human MEK2 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose, activated using c-Raf and then re-purified using Ni2+/NTA agarose.

Specification

• Description: MEK2 protein is a dual specificity protein kinase that belongs to the MAP Kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP Kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax. It catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.40mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 3954U/mg, where one unit of MEK2, active activity is defined as the amount of MEK2 which activates 1μM MAPK2 by 1 unit per minute at 30 centigrade using 100μM ATP. One unit of MAPK2 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 46 kDa
• AA Sequence: 1 MHHHHHHEFM LARRKPVLPA LTINPTIAEG PSPTSEGASE ANLVDLQKKL EELELDEQQK 61 KRLEAFLTQK AKVGELKDDD FERISELGAG NGGVVTKVQH RPSGLIMARK LIHLEIKPAI 121 RNQIIRELQV LHECNSPYIV GFYGAFYSDG EISICMEHMD GGSLDQVLKE AKRIPEEILG 181 KVSIAVLRGL AYLREKHQIM HRDVKPSNIL VNSRGEIKLC DFGVSGQLID SMANSFVGTR 241 SYMAPERLQG THYSVQSDIW SMGLSLVELA VGRYPIPPPD AKELEAIFGR PVVDGEEGEP 301 HSISPRPRPP GRPVSGHGMD SRPAMAIFEL LDYIVNEPPP KLPNGVFTPD FQEFVNKCLI 361 KNPAERADLK MLTNHTFIKR SEVEEVDFAG WLCKTLRLNQ PGTPTRTAV
• Purity: Purity 95% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP2K2 mitogen-activated protein kinase kinase 2 [ Homo sapiens (human) ]
• Official Symbol: MAP2K2
• Synonyms: MAP2K2; mitogen-activated protein kinase kinase 2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
• Gene ID: 5605
• mRNA Refseq: NM_030662.4
• Protein Refseq: NP_109587.1
• MIM: 601263
• UniProt ID: P36507