Active Recombinant Human MAP3K9(134-414) Protein, N-His-tagged

• Cat.No.: MAP3K9-152H
• Product Overview: N-terminal 6Histagged, recombinant, human MLK1, amino acids 134–414, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MLK1, also known as MAP3K9 (Mitogen-Activated Protein Kinase kinase Kinase 9), is a protein coding gene. It enables protein serine/threonine kinase activity and is involved in protein autophosphorylation. It acts as an essential component of the MAP kinase signal transduction pathway and also plays an important role in the cascades of cellular responses evoked by changes in the environment.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 134-414 aa
• Form: 0.59mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 293U/mg, where one unit of MLK1 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 35.3 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF IQLLEIDFAE LTLEEIIGIG GFGKVYRAFW 61 IGDEVAVKAA RHDPDEDISQ TIENVRQEAK LFAMLKHPNI IALRGVCLKE PNLCLVMEFA 121 RGGPLNRVLS GKRIPPDILV NWAVQIARGM NYLHDEAIVP IIHRDLKSSN ILILQKVENG 181 DLSNKILKIT DFGLAREWHR TTKMSAAGTY AWMAPEVIRA SMFSKGSDVW SYGVLLWELL 241 TGEVPFRGID GLAVAYGVAM NKLALPIPST CPEPFAKLME DCWNPDPHSR PSFTNILDQL 301 TTIEESGFFE M
• Purity: Purity 77% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP3K9 mitogen-activated protein kinase kinase kinase 9 [ Homo sapiens (human) ]
• Official Symbol: MAP3K9
• Synonyms: MAP3K9; mitogen-activated protein kinase kinase kinase 9; MLK1; MEKK9; PRKE1
• Gene ID: 4293
• mRNA Refseq: NM_033141.4
• Protein Refseq: NP_149132.2
• MIM: 600136
• UniProt ID: P80192