ATPases Proteins

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ATPases Proteins

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ATPases Proteins Background

ATPase, also known as adenosine triphosphate, is a class of enzymes that can catalyze the hydrolysis of adenosine triphosphate (ATP) into adenosine diphosphate (ADP) and phosphate ions. This is a reaction that releases energy. In most cases, energy can be used to drive another chemical reaction that requires energy. This process is widely used by all known life forms.

Figure 1. ATPase.


ATP is the abbreviation of adenosine triphosphate, and it is a kind of high-energy phosphate compound that exists in various living cells. A high-energy phosphate compound refers to a phosphate compound with an energy released above 20.92 kJ / mol (kilojoules per mole) during hydrolysis, and an energy released during hydrolysis of ATP up to 30.54 kJ / mol. The molecular formula of ATP can be abbreviated as A-P ~ P ~ P. A in the short form represents adenosine, P represents a phosphate group, and ~ represents a special chemical bond, called a high-energy phosphate bond. The hydrolysis of ATP actually refers to the hydrolysis of high-energy phosphate bonds in ATP molecules. When the high-energy phosphate bond is hydrolyzed, a large amount of energy can be released, and a large amount of chemical energy in the ATP molecule is stored in the high-energy phosphate bond. Some ATPases are intrinsic membrane proteins that can be anchored on biofilms and can move on the membranes; these ATPases are also called transmembrane ATPases.

Figure 2. ATP synthase.

Reaction mechanism

The transfer of ATPase coupled with ATP hydrolysis reaction is a strict chemical reaction, that is, each molecule of ATP hydrolysis can transfer a certain number of solution molecules. For example, for sodium-potassium ATPase, each molecule of ATP hydrolysis enables 3 sodium ions to be transported out of the cell and 2 potassium ions to be transported at the same time. Transmembrane ATPases require the energy produced by the hydrolysis of ATP, because these enzymes need work: they transport materials in a direction that is more likely to occur thermodynamically, in other words, using the membrane as a reference, they can move substances from low concentrations while transporting to the high concentration side. This process is called active transportation.

Figure 3. Reaction mechanism.


Transmembrane ATPase can input many substances needed for metabolism and export poisons, metabolic wastes and other substances that may hinder cell processes. For example, sodium-potassium ATPase (also known as sodium / potassium ion ATPase) can regulate the concentration of sodium / potassium ions in the cell to maintain the resting potential of the cell; hydrogen potassium ATPase (also known as hydrogen / potassium ion ATPase) Or gastric proton pump) can keep the acidified environment in the stomach. In addition to acting as ion exchangers, there are other classes of transmembrane ATPases, including co-transporters and "pumps" (some of which are also called "pumps"). Some of these transmembrane ATPases can cause the flow of charge inside and outside the membrane, while others cannot. Therefore, these transporters can be divided into electrogenic and non-electrogenic types.

Figure 4. Function diagram of sodium potassium potassium ATPase.

ATP synthase

ATP synthase is a kind of mitochondria and chloroplast synthetase. It is widely present in mitochondria, chloroplasts, prokaryotes, heterotrophs and photosynthetic bacteria. It is a key enzyme for energy metabolism in organisms. ATP synthetase can use ADP and Pi to catalyze the synthesis of the energy "currency" of living organisms-ATP, driven by the transmembrane proton potential. In general, most of the ATP the body needs is produced by ATP synthase. It is estimated that the amount of ATP the human body needs for normal daily activities is approximately equal to his weight. For example, an adult weighing 70 kilograms synthesizes about 70 kilograms of ATP for normal life activities. And such a huge amount of ATP is synthesized by the body's numerous ATP synthase enzymes. At the same time, ATP synthase can also catalyze the reverse reaction, that is, the hydrolysis of ATP. Therefore, in a sense, ATP synthase is also a type of ATPase.

Figure 5. Structure of ATP synthase.


1. Riley MV.; et al. The localization of the anion-sensitive ATPase activity in corneal endothelium. Biochimica et Biophysica Acta. 1981, 644 (2): 251–6.

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