What are the consequences of genetic mutations preventing the production of a full-length protein of ACBD6?
Genetic mutations of the ACBD6 gene preventing the production of a full-length protein are associated with neurodegenerative syndromes in humans.
What happens to human cells in the absence of ACBD6?
HeLa ACBD6.KO cells that do not produce the ACBD6 protein are viable, but the acylation of lipids is significantly reduced which leads to a defect in formation of lipid droplets. The deficiency of the myristoylation reaction in the absence of an NMT/ACBD6 complex is evidenced by a decrease of the in vivo rate of protein myristoylation, and by the increased sensitivity of the cells to NMT inhibitors.
Is the Recombinant Human ACBD6 Protein suitable for use in protein crystallography studies?
Yes, our recombinant ACBD6 protein may be suitable for crystallization studies or other structural biology applications, but this will depend on the specific protein fragment, preparation, and experimental conditions.
Can the Recombinant ACBD6 Protein be used for diagnostic purposes?
It may have potential application in developing diagnostic assays. However, our recombinant proteins are for research use only and not for clinical use.
What is the recommended amount of recombinant ACBD6 protein to use in experiments?
The recommended amount of recombinant ACBD6 protein to use in experiments varies depending on the specific application and experiment design.
What is the recommended level of endotoxin for the Recombinant ACBD6 Protein?
The recommended level of endotoxin for the Recombinant ACBD6 Protein varies depending on the specific application and experimental requirements. Typically, however, it is recommended to use a protein with endotoxin levels of less than 0.1 EU/μg. Creative BioMart supports recombinant protein production with different endotoxin levels.
Is ACBD6 expressed in all cell types?
ACBD6 expression has been detected in a wide range of cell types, including liver, kidney, heart, and brain tissues. However, the level of expression may vary depending on the cell type and physiological conditions.
Can the recombinant ACBD6 protein be used to generate antibodies?
Yes, the recombinant ACBD6 protein can be used to generate antibodies, which can be used for a variety of applications, including Western blotting, ELISA, and immunohistochemistry. However, it is important to optimize the experimental conditions for antibody generation, such as the choice of adjuvants and the immunization protocol, to obtain high-quality antibodies with the desired specificity and sensitivity.
What applications is the Recombinant Human ACBD6 suitable for?
It is suitable for a variety of applications, including biochemical assays, enzyme kinetics studies, protein-protein interaction studies, and structural biology studies.
Is there any alternative or related protein that can be used as a control?
Yes, there are several alternative or related proteins that can be used as a control when using the recombinant ACBD6 protein in experiments. For example, a control protein with similar biochemical properties.