Recombinant Human ACBD6 cell lysate

Cat.No. : ACBD6-688HCL
Product Overview : Human ACBD6 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : ACBD6 acyl-CoA binding domain containing 6 [ Homo sapiens ]
Official Symbol : ACBD6
Synonyms : ACBD6; acyl-CoA binding domain containing 6; acyl Coenzyme A binding domain containing 6; acyl-CoA-binding domain-containing protein 6; MGC2404; acyl-Coenzyme A binding domain containing 6;
Gene ID : 84320
mRNA Refseq : NM_032360
Protein Refseq : NP_115736
UniProt ID : Q9BR61
Chromosome Location : 1q25.1
Function : binding; fatty-acyl-CoA binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What are the consequences of genetic mutations preventing the production of a full-length protein of ACBD6? 12/06/2022

Genetic mutations of the ACBD6 gene preventing the production of a full-length protein are associated with neurodegenerative syndromes in humans.

What happens to human cells in the absence of ACBD6? 11/30/2022

HeLa ACBD6.KO cells that do not produce the ACBD6 protein are viable, but the acylation of lipids is significantly reduced which leads to a defect in formation of lipid droplets. The deficiency of the myristoylation reaction in the absence of an NMT/ACBD6 complex is evidenced by a decrease of the in vivo rate of protein myristoylation, and by the increased sensitivity of the cells to NMT inhibitors.

Is the Recombinant Human ACBD6 Protein suitable for use in protein crystallography studies? 07/19/2022

Yes, our recombinant ACBD6 protein may be suitable for crystallization studies or other structural biology applications, but this will depend on the specific protein fragment, preparation, and experimental conditions.

Can the Recombinant ACBD6 Protein be used for diagnostic purposes? 06/08/2020

It may have potential application in developing diagnostic assays. However, our recombinant proteins are for research use only and not for clinical use.

What is the recommended amount of recombinant ACBD6 protein to use in experiments? 02/16/2020

The recommended amount of recombinant ACBD6 protein to use in experiments varies depending on the specific application and experiment design.

What is the recommended level of endotoxin for the Recombinant ACBD6 Protein? 06/03/2019

The recommended level of endotoxin for the Recombinant ACBD6 Protein varies depending on the specific application and experimental requirements. Typically, however, it is recommended to use a protein with endotoxin levels of less than 0.1 EU/μg. Creative BioMart supports recombinant protein production with different endotoxin levels.

Is ACBD6 expressed in all cell types? 04/22/2019

ACBD6 expression has been detected in a wide range of cell types, including liver, kidney, heart, and brain tissues. However, the level of expression may vary depending on the cell type and physiological conditions.

Can the recombinant ACBD6 protein be used to generate antibodies? 02/11/2019

Yes, the recombinant ACBD6 protein can be used to generate antibodies, which can be used for a variety of applications, including Western blotting, ELISA, and immunohistochemistry. However, it is important to optimize the experimental conditions for antibody generation, such as the choice of adjuvants and the immunization protocol, to obtain high-quality antibodies with the desired specificity and sensitivity.

What applications is the Recombinant Human ACBD6 suitable for? 12/01/2018

It is suitable for a variety of applications, including biochemical assays, enzyme kinetics studies, protein-protein interaction studies, and structural biology studies.

Is there any alternative or related protein that can be used as a control? 11/26/2017

Yes, there are several alternative or related proteins that can be used as a control when using the recombinant ACBD6 protein in experiments. For example, a control protein with similar biochemical properties.

Customer Reviews (3)

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Reviews
02/14/2021

    The quality of the recombinant human ACBD6 protein from Creative BioMart is exceptional. I highly recommend it to anyone conducting research in this field.

    11/15/2020

      I purchased the recombinant ACBD6 protein from Creative BioMart and it was reasonably priced compared to other suppliers. The fast delivery and great customer service were a bonus.

      08/01/2019

        The technical support team at Creative BioMart was very helpful in assisting me with my order of the Recombinant ACBD6 Protein. Their expertise was invaluable.

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