Active Recombinant Full Length Human MAP3K2 Protein, N-His-tagged

• Cat.No.: MAP3K2-108HFL
• Product Overview: N-terminal 6His-tagged, recombinant, human MEKK2, full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: MEKK2 is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.46mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 118U/mg, where one unit of MEKK2, active activity is defined as 1nmol phosphate incorporated into 1mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 74 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MDDQQALNSI MQDLAVLHKA SRPALSLQET 61 RKAKSSSPKK QNDVRVKFEH RGEKRILQFP RPVKLEDLRS KAKIAFGQSM DLHYTNNELV 121 IPLTTQDDLD KAVELLDRSI HMKSLKILLV INGSTQATNL EPLPSLEDLD NTVFGAERKK 181 RLSIIGPTSR DRSSPPPGYI PDELHQVARN GSFTSINSEG EFIPESMDQM LDPLSLSSPE 241 NSGSGSCPSL DSPLDGESYP KSRMPRAQSY PDNHQEFSDY DNPIFEKFGK GGTYPRRYHV 301 SYHHQEYNDG RKTFPRARRT QGTSLRSPVS FSPTDHSLST SSGSSIFTPE YDDSRIRRRG 361 SDIDNPTLTV MDISPPSRSP RAPTNWRLGK LLGQGAFGRV YLCYDVDTGR ELAVKQVQFD 421 PDSPETSKEV NALECEIQLL KNLLHERIVQ YYGCLRDPQE KTLSIFMEYM PGGSIKDQLK 481 AYGALTENVT RKYTRQILEG VHYLHSNMIV HRDIKGANIL RDSTGNVKLG DFGASKRLQT 541 ICLSGTGMKS VTGTPYWMSP EVISGEGYGR KADIWSVACT VVEMLTEKPP WAEFEAMAAI 601 FKIATQPTNP KLPPHVSDYT RDFLKRIFVE AKLRPSADEL LRHMFVHYH
• Purity: Purity 87% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP3K2 mitogen-activated protein kinase kinase kinase 2 [ Homo sapiens (human) ]
• Official Symbol: MAP3K2
• Synonyms: MAP3K2; mitogen-activated protein kinase kinase kinase 2; MEKK2; MEKK2B
• Gene ID: 10746
• mRNA Refseq: NM_006609.5
• Protein Refseq: NP_006600.3
• MIM: 609487
• UniProt ID: Q9Y2U5