Active Recombinant Full Length Human MAP4K5 Protein, N-GST-tagged

• Cat.No.: MAP4K5-102HFL
• Product Overview: N-terminal GST-tagged, recombinant, full length, human MAP4K5, expressed by baculovirus in Sf21 insect cells. Purified using glutathione-agarose.

Specification

• Description: MAP4K5 is a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: Full Length
• Form: 0.54 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 705 U/mg, where one unit of MAP4K5 activity is defined as 1 nmol phosphate incorporated into 0.33 mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 122 kDa
• Purity: 42% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP4K5 mitogen-activated protein kinase kinase kinase kinase 5 [ Homo sapiens (human) ]
• Official Symbol: MAP4K5
• Synonyms: MAP4K5; mitogen-activated protein kinase kinase kinase kinase 5; KHS; GCKR; KHS1; MAPKKKK5
• Gene ID: 11183
• mRNA Refseq: NM_006575.6
• Protein Refseq: NP_006566.2
• MIM: 604923
• UniProt ID: Q9Y4K4