Active Recombinant Human MAP4K3(1-291) Protein, N-GST,C-His-tagged

• Cat.No.: MAP4K3-129H
• Product Overview: N-terminal GST-tagged and Cterminal 6His tagged, recombinant, human MAP4K3 amino acids 1-291, expressed by baculovirus in Sf21 insect cells. Purified using using immobilized metal affinity chromatography followed by glutathione agarose.

Specification

• Description: MAP4K3 is a member of the mitogen-activated protein Kinase kinase family. The encoded protein activates key effectors in cell Signaling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. Among its related pathways are TGF-Beta Pathway and Angiopoietin-like protein 8 regulatory pathway. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAP4K5.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 1-291 aa
• Form: 0.44 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 804 U/mg, where one unit of MAP4K3 activity is defined as 1 nmol phosphate incorporated into 250 μM RLGRDKYKTLRQIRQ per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 64 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHNQT SLYKKAGTAM 241 NPGFDLSRRN PQEDFELIQR IGSGTYGDVY KARNVNTGEL AAIKVIKLEP GEDFAVVQQE 301 IIMMKDCKHP NIVAYFGSYL RRDKLWICME FCGGGSLQDI YHVTGPLSEL QIAYVSRETL 361 QGLYYLHSKG KMHRDIKGAN ILLTDNGHVK LADFGVSAQI TATIAKRKSF IGTPYWMAPE 421 VAAVERKGGY NQLCDLWAVG ITAIELAELQ PPMFDLHPMR ALFLMTKSNF QPPKLKDKMK 481 WSNSFHHFVK MALTKNPKKR PTAEKLLQHP FVTQHLTRSL AIELLDKVNN ENLYFQGEAC 541 QLGTDDYDIP TTHHHHHH
• Purity: Purity 86% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP4K3 mitogen-activated protein kinase kinase kinase kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MAP4K3
• Synonyms: MAP4K3; mitogen-activated protein kinase kinase kinase kinase 3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• Gene ID: 8491
• mRNA Refseq: NM_003618.4
• Protein Refseq: NP_003609.2
• MIM: 604921
• UniProt ID: Q8IVH8