Active Recombinant Human MAP4K3 protein, GST-tagged

• Cat.No.: MAP4K3-392H
• Product Overview: Recombinant Human MAP4K3(1-380 aa) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Description: GLK is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. GLK is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-and can lead to the activation of the JNK signaling pathway. GLK can directly activate PKC-θ during TCR signaling and people with systemic lupus erythematosus show considerable enhanced GLK expression and activation of PKC-θ and the kinase IKK in T cells. GLK can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 92 nmol/min/mg
• Molecular Mass: ~70 kDa
• Purity: > 70%
• Applications: Kinase Assay, Western Blot
• Stability: 1 year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05μg/μl

Gene Information

• Gene Name: MAP4K3 mitogen-activated protein kinase kinase kinase kinase 3 [ Homo sapiens ]
• Official Symbol: MAP4K3
• Synonyms: MAP4K3; mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1; GLK; MAPKKKK3; MEK kinase kinase 3; MAPK/ERK kinase kinase kinase 3; germinal center kinase-like kinase; germinal center kinase-related protein kinase; MEKKK 3
• Gene ID: 8491
• mRNA Refseq: NM_003618
• Protein Refseq: NP_003609
• MIM: 604921
• UniProt ID: Q8IVH8
• Chromosome Location: 2p22.3
• Pathway: Insulin Signaling, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; TNF receptor signaling pathway, organism-specific biosystem
• Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; small GTPase regulator activity