Recombinant Human MAP3K5, GST-tagged, Active

• Cat.No.: MAP3K5-245H
• Product Overview: Recombinant human ASK1(649-946) was expressed by baculovirus inSf9 insect cellsusing a N-terminal GST tag. MW = 60 kDa.

Specification

• Cat. No.: MAP3K5-245H
• Description: ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6. Overexpression of ASK1 induces apoptotic cell death, and ASK1 is activated in cells treated with tumor necrosis factor-alpha. ASK1 interacts with members of the TRAF family and is activated by TRAF2 in the TNF-signaling pathway. After activation by TRAF2, ASK1 activates MKK4, which in turn activates JNK. Thus, ASK1 is a mediator of TRAF2-induced JNK activation.
• Source: Sf9 insect cells using baculovirus.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Protein length: 649-946

Gene Information

• Gene Name: MAP3K5 mitogen-activated protein kinase kinase kinase 5 [ Homo sapiens ]
• Synonyms: mitogen-activated protein kinase kinase kinase 5; ASK1; MEKK5; MAPKKK5; MAP3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; apoptosis signal regulating kinase 1; ASK-1; EC 2.7.11.25; OTTHUMP00000017275; Apoptosis signal-regulating kinase 1; MEK kinase 5
• Gene ID: 4217
• mRNA Refseq: NM_005923
• Protein Refseq: NP_005914
• MIM: 602448
• UniProt ID: Q99683
• Chromosome Location: 6q22.33
• Pathway: Amyotrophic lateral sclerosis (ALS); MAPK signaling pathway; Neurotrophin signaling pathway
• Function: MAP kinase kinase kinase activity; protein serine/threonine kinase activity; protein homodimerization activity; ATP binding; caspase activator activity; magnesium ion binding; nucleotide binding; transferase activity

Reviews

06/30/2020

The MAP3K5 protein has proven to be invaluable in protein electron microscopy structure analysis.

07/24/2018

Its ability to provide accurate and reliable results in this particular application makes it a preferred choice for researchers studying antigen-antibody interactions and immune responses.

12/01/2016

The MAP3K5 protein's sensitivity and specificity ensure precise measurements, contributing to the overall success of ELISA experiments.

Q&As

What other diseases or conditions involve MAP3K5?

MAP3K5 has been implicated in various diseases, including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions.

How is MAP3K5 protein implicated in cancer?

Aberrant regulation of MAP3K5 has been associated with cancer development, as it can influence cell survival and death pathways.

Can MAP3K5 be used as a biomarker for certain diseases?

MAP3K5 expression levels and activity may serve as biomarkers for specific diseases, providing valuable information for diagnosis and prognosis.

Are there any clinical applications of MAP3K5 in cancer treatment?

Research suggests that targeting MAP3K5 could have therapeutic implications in cancer treatment, potentially through the modulation of apoptosis in cancer cells.

Are there ongoing clinical trials involving MAP3K5 inhibitors?

Investigational studies and clinical trials are underway to explore the efficacy of MAP3K5 inhibitors in different disease contexts, including cancer.