Recombinant Full Length Human MAPK3 Protein, C-Flag-tagged

• Cat.No.: MAPK3-635HFL
• Product Overview: Recombinant Full Length Human MAPK3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 43 kDa
• AA Sequence: MAAAAAQGGGGGEPRRTEGVGPGVP GEVEMVKGQPFDVGPRYTQLQYIGE GAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRF RHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSN DHICYFLYQILRGLKYIHSANVLHR DLKPSNLLINTTCDLKICDFGLARI ADPEHDHTGFLTEYVATRWY RAPEIMLNSKGYTKSIDIWSVGCIL AEMLSNRPIFPGKHYLDQLNHILGI LGSPSQEDLNCIINMKARNY LQSLPSKTKVAWAKLFPKSDSKALD LLDRMLTFNPNKRITVEEALAHPYL EQYYDPTDEPVAEEPFTFAM
  ELDDLPKERLKELIFQETARFQPGV LEAPTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Alzheimer's disease, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, TGF-beta signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction, VEGF signaling pathway

Gene Information

• Gene Name: MAPK3 mitogen-activated protein kinase 3 [ Homo sapiens (human) ]
• Official Symbol: MAPK3
• Synonyms: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Gene ID: 5595
• mRNA Refseq: NM_002746.3
• Protein Refseq: NP_002737.2
• MIM: 601795
• UniProt ID: P27361