NME1

  • Official Full Name

    NME/NM23 nucleoside diphosphate kinase 1

  • Overview

    This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of A (encoded by this gene) and B (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008]
  • Synonyms

    NME1; NME/NM23 nucleoside diphosphate kinase 1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1; nucleoside diphosphate kinase A; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; non-metastatic cells 1, protein (NM23A) expressed in;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • E.coli
  • HEK293
  • HEK293T
  • In Vitro Cell Free System
  • Mammalian Cell
  • Mammalian cells
  • Wheat Germ
  • Flag
  • GST
  • His
  • His (Fc)
  • Avi
  • Myc
  • DDK
  • N/A
  • N
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human NME1-192H Active Recombinant Human NME1 protein E.coli N/A
Human NME1-1310H Recombinant Human NME1, GST-tagged E.coli GST 1-152aa
Human NME1-485H Recombinant Human Non-metastatic Cells 1, Protein (NM23A) E.coli N/A
Human NME1-478H Recombinant Human NME1 protein, His-tagged E.coli His Ala 2 - Glu 152
Human NME1-748H Recombinant Human NME1 Protein, His-tagged E.coli His
Human NME1-28097TH Recombinant Human NME1 E.coli N/A
Human NME1-3792HCL Recombinant Human NME1 293 Cell Lysate HEK293 N/A
Human NME1-5924H Recombinant Human NME1 Protein, GST-tagged Wheat Germ GST
Human NME1-5191H Recombinant Human NME1 protein, GST-tagged E.coli GST 2-152aa
Human NME1-3473H Recombinant Human NME1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc/DDK
Human NME1-1519H-B Recombinant Human NME1 Protein Pre-coupled Magnetic Beads HEK293
Human NME1-6571HF Recombinant Full Length Human NME1 Protein, GST-tagged In Vitro Cell Free System GST 152 amino acids
Human NME1-1519H Recombinant Human NME1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human NME1-4709H Recombinant Human NME1 Protein (Met1-Glu152), N-His tagged E.coli N-His Met1-Glu152
Human NME1-910HFL Recombinant Full Length Human NME1 Protein, C-Flag-tagged Mammalian cells Flag
Mouse NME1-10738M Recombinant Mouse NME1 Protein Mammalian Cell His
Mouse NME1-6108M-B Recombinant Mouse NME1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Nme1-1719M Recombinant Mouse Nme1 protein, His-tagged E.coli His Arg6~Gln147
Mouse NME1-6108M Recombinant Mouse NME1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat NME1-4003R Recombinant Rat NME1 Protein Mammalian Cell His
Rat NME1-3662R Recombinant Rat NME1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat NME1-3662R-B Recombinant Rat NME1 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque NME1-3054R Recombinant Rhesus monkey NME1 Protein, His-tagged Mammalian Cell His
Rhesus Macaque NME1-2873R-B Recombinant Rhesus Macaque NME1 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque NME1-2873R Recombinant Rhesus Macaque NME1 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Chicken NME1-4500C Recombinant Chicken NME1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
Schematic representation of four common functions of Nm23-H1.jpg

Fig1. Schematic representation of four common functions of Nm23-H1. (Saurabh Pandey, 2018)

What is NME1 protein?

NME1 (NME/NM23 nucleoside diphosphate kinase 1) gene is a protein coding gene which situated on the long arm of chromosome 17 at locus 17q21. NME1, full name NME/NM23 nucleoside diphosphokinase 1 (NME1), is a multifunctional enzyme that plays an important role in inhibiting tumor cell invasion and metastasis. This protein is evolutionarily conserved and plays a role in a variety of biological processes, including cell proliferation, differentiation, development, signal transduction, endocytosis of G protein-coupled receptors, and gene expression. Expression levels of NME1 are reduced in highly metastasized cells, where it exists as A hexamer composed of subunits A (encoded by the NME1 gene) and B (encoded by the NME2 gene). NME1 protein shows cytoplasmic expression in a variety of tissues and has low tissue specificity in most tissues. The NME1 protein is consisted of 152 amino acids and its molecular mass is approximately 17.1 kDa.

What is the function of NME1 protein?

One of the main functions of NME1 is as a nucleoside diphosphokinase (NDPK), which is involved in the interconversion between the cell kernel side diphosphate (NDP) and triphosphate (NTP) by transferring the α-phosphate group. This activity is essential for maintaining the balance of ATP and other key nucleotides within the cell. By influencing the level and distribution of ATP in cells, NME1 is involved in the regulation of cytoskeleton rearrangement and cell motility, which has important significance for cell migration and invasion ability. NME1 plays a role in the endocytosis of G protein-coupled receptors, which involves the uptake of substances mediated by receptors on the cell surface. In addition, NME1 plays a role in cells responding to oxidative and metabolic stress.

NME1 Related Signaling Pathway

NME1 (also known as nm23-H1) is a widely expressed, multifunctional protein that plays a key role in many biological processes. NME1 is mainly involved in a variety of signaling pathways as a nucleoside diphosphate kinase (NDPK), and its core function is to catalyze the conversion of nucleoside diphosphate (NDPs) to the corresponding triphosphate (NTPs), so as to maintain the balance of intracellular nucleotide pools. NME1 is able to influence cell proliferation and survival by regulating the activity of the Raf-MEK-ERK pathway, which is an important signal transduction pathway in many types of cancer. NME1 interacts with the PI3K/Akt pathway, which plays a key role in cell survival, growth, and metabolism.

NME1 Related Diseases

Mutations in the NME1 gene have been identified in neuroblastoma, which originates from neural crest cells and is one of the most common solid tumors in children. Low expression of the NME1 gene has been associated with advanced metastasis of a variety of epithelial cancers, including melanoma, a malignant tumor that originates in melanocytes. It is also associated with a variety of cancers including breast cancer, colorectal cancer and liver cancer. NME1 protects against neurotoxin, alpha-synuclein, and LRRK2-induced neuromutation in a cell model of Parkinson's disease, suggesting that NME1 may play a role in neuroprotection.

Bioapplications of NME1

Because NME1 is associated with tumorigenesis, development, and metastasis, researchers often study its expression patterns and function in different types of cancer. These studies contribute to understanding the molecular mechanisms of cancer and may provide new targets for cancer treatment. A deeper understanding of NME1's function could lead to the development of new drugs, particularly for diseases where NME1 expression or activity is abnormal. For example, the development of small molecule drugs that can regulate the activity of NME1 may be beneficial in the treatment of certain cancers. The detection of NME1 can serve as a cognitive intelligence model segment to help physicians assess disease states, especially in cancer management.

High Purity

SDS-PAGE (NME1-5924H).jpg

Fig1. SDS-PAGE (NME1-5924H)

.

SDS-PAGE (NME1-5191H).jpg

Fig2. SDS-PAGE (NME1-5191H)

Case Study 1: Imran Khan, 2022

Tumor-derived exosomes have documented roles in accelerating the initiation and outgrowth of metastases, as well as in therapy resistance. Little information supports the converse, that exosomes or similar vesicles can suppress metastasis. The researchers investigated the NME1 (Nm23-H1) metastasis suppressor as a candidate for metastasis suppression by extracellular vesicles. Exosomes derived from two cancer cell lines (MDA-MB-231T and MDA-MB-435), when transfected with the NME1 (Nm23-H1) metastasis suppressor, secreted exosomes with NME1 as the predominant constituent. These exosomes entered recipient tumor cells, altered their endocytic patterns in agreement with NME1 function, and suppressed in vitro tumor cell motility and migration compared to exosomes from control transfectants. Proteomic analysis of exosomes revealed multiple differentially expressed proteins that could exert biological functions. Therefore, they also prepared and investigated liposomes, empty or containing partially purified rNME1. rNME1 containing liposomes recapitulated the effects of exosomes from NME1 transfectants in vitro.

MDA-MB-231T cells were treated with exosomes.jpg

Fig1. Naïve MDA-MB-231T cells were treated with exosomes derived from vector- or NME1 overexpressing MDA-MB-435 or MDA-MB-231T cells and total cellular NME1 expression was assessed by western blots.

Control (empty) liposomes and liposomes containing partially purified recombinant rNME1 were assessed.jpg

Fig2. Control (empty) liposomes and liposomes containing partially purified recombinant rNME1 were assessed for particle size distributions and zeta potential using ZetaView.

Case Study 2: Jin Hyoung Cho, 2021

This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, this study demonstrates that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, the researchers produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs.

The schematic structure and production of rhNME1 or His-rhNME1 proteins.jpg

Fig3. The schematic structure and production of rhNME1 or His-rhNME1 proteins.

Phase-contrast images of cultured mp AD-MSCs and NI-mp AD-MSCs with or without rhNME1.jpg

Fig4. Phase-contrast images of cultured mp AD-MSCs and NI-mp AD-MSCs with or without rhNME1 (4 ng/mL).

NME1 involved in several pathways and played different roles in them. We selected most pathways NME1 participated on our site, such as Purine metabolism, Pyrimidine metabolism, Metabolic pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with NME1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Purine metabolismPOLE4;POLR3GLB;PAPSS2;NT5C1A;POLR2F;PDE6D;POLR3E;CANT1;POLA1
Pyrimidine metabolismCANT1;DHODH;UPB1;PNP5A;UPP1;CANT1A;PNPT1;UCK2B;POLR2K
Metabolic pathwaysPSAT1;CHPF2;PCK2;NDUFA11;SI;NDUFS3;SYNJ2;COMTA;ATP6V0A1B

NME1 has several biochemical functions, for example, ATP binding, GTP binding, deoxyribonuclease activity. Some of the functions are cooperated with other proteins, some of the functions could acted by NME1 itself. We selected most functions NME1 had, and list some proteins which have the same functions with NME1. You can find most of the proteins on our site.

Function Related Protein
ATP bindingNLRX1;HSPA12B;PMS2;MKNK2;ATAD5;TNIKB;NIM1K;ACSM4;CSNK1A1L
GTP bindingARL4AB;SEPT10;EEF1A1B;MOCS1;GPN2;GMPPAA;LGALS6;GBP2;NUDT2
deoxyribonuclease activityDNASE1L3;DNASE1L2;TATDN3;TATDN1;DNASE1L3L;ENDOG;TATDN2;NME1;DNASE1L1
identical protein bindingSF1;AGXT2;MMP9;NUP153;EWSR1;DHX8;TRIP13;RAD52;CPT1A
magnesium ion bindingLATS1;PGM3;ATP8B3;ABL2;ATP4A;CIB3;ALPP;NT5C3A;HPRT1
nucleoside diphosphate kinase activityNME7;AK8;NME3;AK7;TXNDC3;AK9;NME2B.1;CMPK2;NME8
poly(A) RNA bindingPOLR2A;PFN1;RPS27L;RPL23;TBRG4;CHCHD1;SLC3A2;HRSP12;LUZP4
protein bindingZNF346;PRPF31;SUGT1;ABHD14B;FGR;ZKSCAN1;ADORA2A;MTNR1B;FAM76B
ribosomal small subunit bindingMTIF3;PIM1;PTCD3;NPM1;NME1;DHX29;DDX3X;ERAL1;CPEB2

NME1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with NME1 here. Most of them are supplied by our site. Hope this information will be useful for your research of NME1.

N/A; POLR1C; NME4; NME3

  • Q&As
  • Reviews

Q&As (7)

Ask a question
What role does NME1 play in DNA repair and the maintenance of genomic stability? 12/05/2022

NME1 plays a role in DNA repair, contributing to genomic stability.

How does NME1 influence signal transduction in response to cellular stress? 01/30/2022

NME1 modulates cellular responses to stress, affecting signal transduction pathways.

What is the impact of NME1 on apoptosis and cell survival pathways? 05/09/2020

NME1 influences apoptosis and cell survival, impacting cell fate decisions.

How do alterations in NME1 expression or activity affect drug resistance in cancer therapy? 11/05/2019

Changes in NME1 can impact drug resistance in cancer, affecting treatment efficacy.

What are the mechanisms by which NME1 modulates the immune response, especially in tumor immunity? 10/21/2018

NME1 regulates the immune response, particularly in tumor immunity.

How does NME1 contribute to the metastatic potential of cancer cells? 07/03/2018

NME1 affects the metastatic behavior of cancer cells, influencing their spread.

How does NME1 regulate nucleotide metabolism and cellular energy balance? 04/20/2018

NME1 is involved in nucleotide metabolism, crucial for maintaining cellular energy balance.

Customer Reviews (3)

Write a review
Reviews
04/02/2022

    Critical expertise in protein engineering, unmatched in quality.

    10/28/2021

      Reliable protein crystallization services, consistently yields exceptional results.

      12/23/2018

        Efficient protein purification, consistent yields, cost-effective for research.

        Ask a Question for All NME1 Products

        Required fields are marked with *

        My Review for All NME1 Products

        Required fields are marked with *

        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends