Recombinant Human NME1 293 Cell Lysate

• Cat.No.: NME1-3792HCL

Specification

• Description: Antigen standard for non-metastatic cells 1, protein (NM23A) expressed in (NME1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NME1 non-metastatic cells 1, protein (NM23A) expressed in [ Homo sapiens ]
• Official Symbol: NME1
• Synonyms: NME1; non-metastatic cells 1, protein (NM23A) expressed in; nucleoside diphosphate kinase A; NM23; NM23 H1; NDP kinase A; granzyme A-activated DNase; metastasis inhibition factor nm23; tumor metastatic process-associated protein; NB; AWD; NBS; GAAD; NDKA; NDPKA; NDPK-A; NM23-H1
• Gene ID: 4830
• mRNA Refseq: NM_000269
• Protein Refseq: NP_000260
• MIM: 156490
• UniProt ID: P15531
• Chromosome Location: 17q21.33
• Pathway: Arf6 downstream pathway, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Purine metabolism, organism-specific biosystem
• Function: ATP binding; GTP binding; deoxyribonuclease activity; gamma-tubulin binding; identical protein binding; kinase activity; magnesium ion binding; nucleoside diphosphate kinase activity; nucleotide binding; protein binding; transferase activity