Active Recombinant Mouse MAP2K4(34-end) Protein, N-GST-tagged

• Cat.No.: MAP2K4-145M
• Product Overview: N-terminal GST-tagged, murine MKK4 residues 34–end, expressed in E.coli cells. Purified using glutathione agarose. Activated by incubation with MEKK and then re-purified using Ni2+/NTA agarose.

Specification

• Description: MKK4 is a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis.
• Source: E. coli
• Species: Mouse
• Tag: GST
• Protein length: 34-end
• Form: 1.553mg/ml of enzyme in 50mM Tris/HCl, pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 607U/mg, where one unit of MKK4 activity is defined as the amount of MKK4 which activates 2μM unactive JNK 1α1 by 1 unit per minute at 30C using 100μM ATP. One unit of JNK 1α1 activity is defined as 1nmol phosphate incorporated into 3μM ATF-2 (19-96) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 67.7 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMQGK RKALKLNFAN 241 PPVKSTARFT LNPNTTGVQN PHIERLRTHS IESSGKLKIS PEQHWDFTAE DLKDLGEIGR 301 GAYGSVNKMV HKPSGQIMAV KRIRSTVDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR 361 EGDCWICMEL MSTSFDKFYK YVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI 421 KPSNILLDRS GNIKLCDFGI SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV 481 WSLGITLYEL ATGRFPYPKW NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK 541 DESKRPKYKE LLKHPFILMY EERTVEVACY VCKILDQMPA TPSSPMYVD
• Purity: Purity 63% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: Map2k4 mitogen-activated protein kinase kinase 4 [ Mus musculus (house mouse) ]
• Official Symbol: MAP2K4
• Synonyms: MAP2K4; mitogen-activated protein kinase kinase 4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4; MAPKK 4
• Gene ID: 26398
• mRNA Refseq: NM_009157.5
• Protein Refseq: NP_033183.1
• UniProt ID: P47809