Recombinant Mouse MAP2K4 cell lysate MAP2K4-557MCL

Recombinant Mouse MAP2K4 cell lysate

PRODUCTS

Home / Products / Cell & Tissue Lysates / Over-Expression Lysates / Recombinant Mouse MAP2K4 cell lysate

Recombinant Mouse MAP2K4 cell lysate Online Inquiry

MAP2K4 Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : MAP2K4-557MCL Optional Service: Optional requirements on this protein
Product Overview : Mouse MAP2K4 / JNKK1 / MKK4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Map2k4 mitogen-activated protein kinase kinase 4 [ Mus musculus ]
Official Symbol : MAP2K4
Synonyms : MAP2K4; mitogen-activated protein kinase kinase 4; dual specificity mitogen-activated protein kinase kinase 4; MEK 4; JNKK 1; MAPKK 4; JNK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; SAPK/Erk/kinase 1; MAP kinase kinase 4; JNK-activating kinase 1; C-JUN N-terminal kinase kinase 1; mitogen activated protein kinase kinase 4, variant 1; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4;
Gene ID : 26398
mRNA Refseq : NM_009157
Protein Refseq : NP_033183
Pathway : Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem;
Function : ATP binding; JUN kinase kinase activity; MAP kinase kinase activity; kinase activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry


  • Note: There will be extra charge for optional service!

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2022 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy