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Recombinant Mouse MAP2K4 cell lysate

Cat.No. : MAP2K4-557MCL
Product Overview : Mouse MAP2K4 / JNKK1 / MKK4 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Map2k4 mitogen-activated protein kinase kinase 4 [ Mus musculus ]
Official Symbol : MAP2K4
Synonyms : MAP2K4; mitogen-activated protein kinase kinase 4; dual specificity mitogen-activated protein kinase kinase 4; MEK 4; JNKK 1; MAPKK 4; JNK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; SAPK/Erk/kinase 1; MAP kinase kinase 4; JNK-activating kinase 1; C-JUN N-terminal kinase kinase 1; mitogen activated protein kinase kinase 4, variant 1; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4;
Gene ID : 26398
mRNA Refseq : NM_009157
Protein Refseq : NP_033183
Pathway : Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem;
Function : ATP binding; JUN kinase kinase activity; MAP kinase kinase activity; kinase activity; mitogen-activated protein kinase kinase kinase binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Are there existing or potential therapeutic strategies targeting MAP2K4? 06/12/2022

Targeting MAP2K4 may provide therapeutic benefits in cancer, modulating cell survival and response to stress.

What role does MAP2K4 play in cellular stress response? 03/24/2022

MAP2K4 helps cells respond to stress, modulating survival and death pathways.

How is MAP2K4 implicated in cancer development and progression? 04/28/2020

Dysregulation of MAP2K4 has been linked to cancer, affecting cell growth and resistance to apoptosis.

How does MAP2K4 contribute to the JNK and p38 MAPK pathways? 02/25/2020

It phosphorylates and activates JNK and p38, transmitting signals in response to stress.

How is the activity of MAP2K4 regulated within cells? 10/29/2019

Its activity is regulated by phosphorylation and interaction with other signaling proteins.

In which cellular processes is MAP2K4 involved? 10/07/2019

MAP2K4 is involved in processes like cell growth, apoptosis, and stress response.

What is the primary biological function of MAP2K4 in cellular signaling? 11/23/2018

MAP2K4 is a kinase that plays a critical role in activating the JNK and p38 MAPK signaling pathways.

Customer Reviews (3)

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Reviews
10/25/2021

    Good value, high quality.

    04/03/2021

      Pure product, no issues.

      03/13/2021

        Reliable, worked as described.

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