Creative BioMart to Present at BPS 2025 Annual Meeting | February 15-19, 2025

DMP1

  • Official Full Name

    dentin matrix acidic phosphoprotein 1

  • Overview

    Dentin matrix protein 1, also known as DMP-1, is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in DMP-1 are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia.
  • Synonyms

    DMP1; dentin matrix acidic phosphoprotein 1; dentin matrix acidic phosphoprotein; dentin matrix protein 1; ARHP; ARHR; DMP-1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Dog
  • Human
  • Mouse
  • E.coli
  • HEK293
  • HEK293T
  • Human Cell
  • Mammalian Cell
  • Mammalian cells
  • Wheat Germ
  • Yeast
  • GST
  • His
  • Fc
  • Avi
  • SUMO
  • Myc
  • DDK
  • Non
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human DMP1-662H Active Recombinant Human DMP1, His-tagged Mammalian cells His Leu17-Tyr497;Asp202-Tyr497;Leu17-Ser201
Human DMP1-2706H Recombinant Human DMP1 Protein, GST-tagged Wheat Germ GST
Human DMP1-503H Recombinant Human DMP1 Mammalian Cell His
Human DMP1-3790H Active Recombinant Human DMP1 protein, His-tagged HEK293 His Met1-Tyr513
Human DMP1-1977HCL Recombinant Human DMP1 cell lysate Human Cell Non
Human DMP1-2803H Recombinant Human DMP1 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Human DMP1-2212H Recombinant Human DMP1 Protein, MYC/DDK-tagged HEK293 Myc&DDK
Human DMP1-1973H Recombinant Human DMP1 Protein (Lys17-Tyr513), C-His tagged Mammalian cells His Lys17-Tyr513
Human DMP1-2803H-B Recombinant Human DMP1 Protein Pre-coupled Magnetic Beads HEK293
Human DMP1-2398H Recombinant Human DMP1 Protein (17-513 aa), His-tagged Yeast His 17-513 aa
Human DMP1-6727H Recombinant Human DMP1 Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293T Myc&DDK
Mouse Dmp1-407M Active Recombinant Mouse Dentin Matrix Protein 1, His-tagged Mammalian cells His Leu17-Tyr503
Mouse DMP1-4647M Recombinant Mouse DMP1 Protein Mammalian Cell His
Mouse Dmp1-2585M Recombinant Mouse Dmp1 Protein, Myc/DDK-tagged HEK293T Myc&DDK
Mouse Dmp1-2412M-B Recombinant Mouse Dmp1 Protein Pre-coupled Magnetic Beads HEK293
Mouse Dmp1-2412M Recombinant Mouse Dmp1 Protein, His (Fc)-Avi-tagged HEK293 His&Fc&Avi
Dog DMP1-2032D Recombinant Dog DMP1 Protein (1-435 aa), His-SUMO-tagged E.coli His&SUMO 1-435 aa
Chicken DMP1-7116C Recombinant Chicken DMP1 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource
The structure of the Dmp1 (Dmtf1) transcription factor.jpg

Fig1. The structure of the Dmp1 (Dmtf1) transcription factor. (K Inoue, 2007)

What is DMP1 protein?

DMP1 (DEAD-box helicase 6) gene is a protein coding gene which situated on the long arm of chromosome 4 at locus 4q22. Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. The DMP1 protein is consisted of 513 amino acids and its molecular mass is approximately 55.8 kDa.

What is the function of DMP1 protein?

DMP1 is essential for the biomineralization of calcium phosphate in bone and dentin. It nucleates the formation of hydroxyapatite, which increases mineralization. DMP1 is crucial for the maturation of odontoblasts and osteoblasts, which are important for bone formation and repair. DMP1 is a major local suppressor of the hormone FGF23 (Fibroblast Growth Factor 23). Inactivating mutations of DMP1 can lead to an overproduction of FGF23, which in turn causes renal phosphate wasting and contributes to conditions like rickets and osteomalacia. DMP1 has a protective role against osteocyte apoptosis, which is particularly important in the context of high circulating phosphate levels. DMP1 is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family, which includes other proteins that play roles in bone mineralization and are involved in various diseases.

DMP1 Related Signaling Pathway

Dmp1-mediated calcium signaling can activate p38 MAPK and promote osteoblast differentiation. DMP1 interacts with the Ras-Raf-MEK-ERK signaling pathway to prevent tumor formation by affecting the Arf/Mdm2/p53 signaling pathway. Lipopolysaccharide affects the proliferation, differentiation and apoptosis of human dental pulp stem cells by regulating Notch signaling pathway, and the expression of DMP1 is related to the activation of this pathway. In rat dental pulp stem cells, DMP1 expression is regulated by the Wnt/β-catenin signaling pathway. DMP1 is involved in the regulation of phosphorus and vitamin D metabolism by influencing the expression and activity of FGF23. DMP1 may interact with the Hedgehog signaling pathway to influence bone development and homeostasis. DMP1 may affect cell proliferation and survival through the PI3K-Akt signaling pathway.

DMP1 Related Diseases

The disease is caused by an inactivated mutation in the DMP1 gene and is characterized by increased kidney phosphorus excretion, resulting in hypophosphatemia and bone mineralization disorders. DMP1 is involved in the pathogenesis of osteoporosis by influencing FGF23 expression and WNT/β-catenin signaling pathway. DMP1 plays a role in the process of fracture healing, and the change of its expression level is related to the rate of fracture healing. Mutations in the DMP1 gene may be associated with certain types of osteosclerosis, a disease in which bones harden and thicken. DMP1 may be associated with osteoporosis associated with thalassemia, manifested by a high rate of bone renewal and increased serum Dkk-1 concentration. DMP1 may play a role in the occurrence and development of bone tumors, and its specific role in tumor bone metastasis needs to be further studied.

Bioapplications of DMP1

DMP1 plays a role in the fracture healing process and may help promote fracture healing by regulating DMP1 activity or expression levels. DMP1 plays a key role in the tooth mineralization process and can be used to develop materials that promote tooth repair and regeneration. DMP1 can be used to develop biologically active bone repair materials that promote bone formation in bone tissue engineering. As a drug target, DMP1 can be used to screen small molecule drugs that can modulate their activity for the treatment of related diseases.

High Purity

SDS-PAGE (DMP1-2706H).jpg

Fig1. SDS-PAGE (DMP1-2706H)

.

SDS-PAGE (DMP1-1973H).jpg

Fig2. SDS-PAGE (DMP1-1973H)

Case Study 1: Aleksandra Porębska, 2020

Dentin matrix protein 1 (DMP1) is an acidic, extracellular matrix protein essential for biomineralization of calcium phosphate, in bone and dentin. It is proteolytically processed into two fragments, 44K and 56K. Recently, the presence of DMP1 was noticed in inner ear, specifically in otoconia, which are calcium carbonate biominerals involved in sensing of balance. In this study, the solution structure and biomineralization activity of otoconial 44K and 56K fragments toward calcium carbonate were investigated. The results of analytical ultracentrifugation, circular dichroism, and gel filtration indicated that DMP1 fragments are disordered in solution. Notably, 56K formed oligomers in the presence of calcium ions. It was also observed that both fragments influenced the crystal growth by in vitro biomineralization assay and scanning electron microscopy. In addition, they sequester the calcium ions during the calcite formation. Calcium carbonate crystals precipitated in vitro changed their size and shape in the presence of DMP1 fragments. Oligomerization propensity of 56K may significantly enhance this function.

The prediction of the disordered regions from an amino acid sequence.jpg

Fig1. The prediction of the disordered regions from an amino acid sequence.

SDS-PAGE analysis of purified human DMP1 fragments.jpg

Fig2. SDS-PAGE analysis of purified human DMP1 fragments 44K (lane 2) and 56K (lane 4).

Case Study 2: Chang-Yun Yoon, 2016

Recent reports demonstrated that dentin matrix protein 1 (DMP1) acts as an inhibitor of vascular calcification and might be a potential biomarker for chronic kidney disease-mineral and bone disorder; however, no clinical investigations regarding DMP1 have been performed in dialysis patients. The researchers investigated the prognostic value of DMP1 on cardiovascular outcomes in prevalent peritoneal dialysis patients. They recruited 223 prevalent peritoneal dialysis patients and divided them into high and low DMP1 groups according to log-transformed plasma DMP1 levels. A Cox proportional hazards analysis determined DMP1 was independently associated with cardiovascular outcomes. In vitro mouse osteocytes were cultured in media containing indoxyl sulfate (IS), and the expressions of DMP1 were examined. The multiple logistic regression analysis indicated that DMP1 levels were independently associated with the presence of vascular calcification after adjustment for multiple confounding factors. A Kaplan-Meier plot showed that the low DMP1 group had a significantly higher rate of incident cardiovascular events compared with the high DMP1 group. In addition, multiple Cox analysis showed that low DMP1 was significantly associated with incident cardiovascular events. In IS-stimulated osteocytes, mRNA and protein expression levels of DMP1 were significantly decreased compared with control osteocytes.

Comparison of the log-transformed median value of DMP1 levels between the PD group and controls by using box plots.jpg

Fig3. Comparison of the log-transformed median value of DMP1 levels between the PD group and controls by using box plots.

Kaplan-Meier plots for CV outcome-free survival between the lower and higher DMP1 groups.jpg

Fig4. Kaplan-Meier plots for CV outcome-free survival between the lower and higher DMP1 groups.

DMP1 involved in several pathways and played different roles in them. We selected most pathways DMP1 participated on our site, such as ECM proteoglycans, Extracellular matrix organization, which may be useful for your reference. Also, other proteins which involved in the same pathway with DMP1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
ECM proteoglycansMATN3B;LRP4;COMP;DMP1;MATN4;DAG1;COL9A1;MUSK;BGNA
Extracellular matrix organizationCOL8A1A;FURINB;COL15A1B;COL4A5;MFAP1B;MMP10;COL19A1;MGC174857;MMP15

DMP1 has several biochemical functions, for example, calcium ion binding, extracellular matrix binding, integrin binding. Some of the functions are cooperated with other proteins, some of the functions could acted by DMP1 itself. We selected most functions DMP1 had, and list some proteins which have the same functions with DMP1. You can find most of the proteins on our site.

Function Related Protein
calcium ion bindingRASGRP1;EFCAB12;MMP15;SULF1;PLA2G2F;SYT3;FAT1A;RYR3;DNASE1L3
extracellular matrix bindingITGA2B;CD248;SPOCK2;SPARC;SID4;DMP1;FBLN2;DCN;VEGFA
integrin bindingITGA5;CD226;GPNMB;CIB2;TGFBI;GFAP;MMP14;COL5A1;THBS4

DMP1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with DMP1 here. Most of them are supplied by our site. Hope this information will be useful for your research of DMP1.

Research Area

Related articles

Sapir-Koren, R; Livshits, G; et al. Osteocyte control of bone remodeling: is sclerostin a key molecular coordinator of the balanced bone resorption-formation cycles?. OSTEOPOROSIS INTERNATIONAL 25:2685-2700(2014).
Sato, S; Hashimoto, J; et al. Novel sandwich ELISAs for rat DMP1: Age-related decrease of circulatory DMP1 levels in male rats. BONE 57:429-436(2013).
  • Reviews
  • Q&As

Customer Reviews (3)

Write a review
Reviews
02/01/2022

    This commitment to excellence ensures that researchers are provided with the most advanced and reliable tools for their investigations.

    12/16/2020

      The DMP1 protein offers numerous advantages in trials and research experiments.

      11/01/2017

        Not only does it provide reliable and consistent results due to its high quality and purity, but it also holds significant value in understanding cellular processes and disease mechanisms.

        Q&As (5)

        Ask a question
        Can DMP1 be a target for drug development? 01/22/2021

        Yes, targeting DMP1 in drug development may offer new avenues for treating conditions related to bone and dentin abnormalities.

        Are there therapeutic applications of DMP1 in bone-related conditions? 10/14/2020

        Research suggests that DMP1 may have therapeutic potential in promoting bone regeneration and treating conditions like osteoporosis.

        How does DMP1 contribute to the repair of bone fractures? 06/06/2020

        DMP1 is involved in the repair and remodeling of bone tissue after fractures by influencing the mineralization and structural integrity of the newly formed bone.

        How can DMP1 be utilized in diagnostic procedures? 11/21/2018

        DMP1 levels can be measured in biological samples, such as blood or saliva, to assess the potential presence of dental or skeletal disorders and to monitor disease progression.

        Is there a connection between DMP1 and periodontal disease? 02/24/2018

        Some studies suggest that DMP1 may play a role in maintaining the health of periodontal tissues, and alterations in its expression could be associated with periodontal disease.

        Ask a Question for All DMP1 Products

        Required fields are marked with *

        My Review for All DMP1 Products

        Required fields are marked with *

        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2025 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends